Results 31 to 40 of about 3,349,944 (177)

Lipoate-Protein Ligase and Octanoyltransferase Are Essential for Protein Lipoylation in Mitochondria of Arabidopsis     [PDF]

Plant Physiology, 2014
AbstractProsthetic lipoyl groups are required for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), and the glycine cleavage system (glycine decarboxylase [GDC]). How these proteins are lipoylated has been extensively studied in prokaryotes and yeast (Saccharomyces ...
Ralph, Ewald, Christiane, Hoffmann, Alexandra, Florian, Ekkehard, Neuhaus, Alisdair R, Fernie, Hermann, Bauwe   +5 more
openaire   +2 more sources

Lipoic Acid Metabolism as a Potential Chemotherapeutic Target Against Plasmodium falciparum and Staphylococcus aureus

Frontiers in Chemistry, 2021
Lipoic acid (LA) is an organic compound that plays a key role in cellular metabolism. It participates in a posttranslational modification (PTM) named lipoylation, an event that is highly conserved and that occurs in multimeric metabolic enzymes of very ...
Sun Liu Rei Yan, Felipe Wakasuqui, Xiaochen Du, Matthew R. Groves, Carsten Wrenger   +4 more
doaj   +1 more source

Chemoselective and laser cleavable probes for <i>in situ</i> protein lipoylation detection by laser desorption/ionization mass spectrometry. [PDF]

Chem Sci
Chemoselective probe is connected with gold nanoparticles modified with laser cleavable mass tags by click chemistry for in situ protein lipoylation detection by laser desorption/ionization mass spectrometry.
Du Q, Yu X, Jia K, Qu Y, Han J, Sun J, Shen D, Liu H, Nie Z.   +8 more
europepmc   +3 more sources

Proteomic analysis reveals a novel function of the kinase Sat4p in Saccharomyces cerevisiae mitochondria. [PDF]

PLoS ONE, 2014
The Saccharomyces cerevisiae kinase Sat4p has been originally identified as a protein involved in salt tolerance and stabilization of plasma membrane transporters, implicating a cytoplasmic localization. Our study revealed an additional mitochondrial (mt)
Uta Gey, Cornelia Czupalla, Bernard Hoflack, Udo Krause, Gerhard Rödel   +4 more
doaj   +1 more source

Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria

eLife, 2020
Cells harbor two systems for fatty acid synthesis, one in the cytoplasm (catalyzed by fatty acid synthase, FASN) and one in the mitochondria (mtFAS).
Sara M Nowinski, Ashley Solmonson, Scott F Rusin, J Alan Maschek, Claire L Bensard, Sarah Fogarty, Mi-Young Jeong, Sandra Lettlova, Jordan A Berg, Jeffrey T Morgan, Yeyun Ouyang, Bradley C Naylor, Joao A Paulo, Katsuhiko Funai, James E Cox, Steven P Gygi, Dennis R Winge, Ralph J DeBerardinis, Jared Rutter   +18 more
doaj   +1 more source

LipoSVM: Prediction of Lysine lipoylation in Proteins based on the Support Vector Machine [PDF]

Current Genomics, 2019
Background:Lysine lipoylation which is a rare and highly conserved post-translational modification of proteins has been considered as one of the most important processes in the biological field. To obtain a comprehensive understanding of regulatory mechanism of lysine lipoylation, the key is to identify lysine lipoylated sites. The experimental methods
Wu, Meiqi, Lu, Pengchao, Yang, Yingxi, Liu, Liwen, Wang, Hui, Xu, Yan, Chu, Jixun   +6 more
openaire   +3 more sources

A Lipoylated Metabolic Protein Released by Staphylococcus aureus Suppresses Macrophage Activation [PDF]

Cell Host & Microbe, 2017
The virulence factors of pathogenic microbes often have single functions that permit immune suppression. However, a proportion possess multiple activities and are considered moonlighting proteins. By examining secreted virulence factors of Staphylococcus aureus, we determine that the bacterial lipoic acid synthetase LipA suppresses macrophage ...
James P, Grayczyk, Cameron J, Harvey, Irina, Laczkovich, Francis, Alonzo   +3 more
openaire   +2 more sources

Studying protein-protein interactions using peptide arrays [PDF]

, 2010
Screening of arrays and libraries of compounds is well-established as a high-throughput method for detecting and analyzing interactions in both biological and chemical systems. Arrays and libraries can be composed from various types of molecules, ranging
Rito, T., Rudiger, S.G.D., Sub Cellular Protein Chemistry, Rotem-Bamberger, S., Katz, C., Friedler, A., Levy-Beladev, L., Cellular Protein Chemistry   +7 more
core   +1 more source

Sirtuin Lipoamidase Activity Is Conserved in Bacteria as a Regulator of Metabolic Enzyme Complexes

mBio, 2017
Lipoic acid is an essential metabolic cofactor added as a posttranslational modification on several multimeric enzyme complexes. These protein complexes, evolutionarily conserved from bacteria to humans, are core regulators of cellular metabolism.
Elizabeth A. Rowland, Todd M. Greco, Caroline K. Snowden, Anne L. McCabe, Thomas J. Silhavy, Ileana M. Cristea   +5 more
doaj   +1 more source

[32] Assay for protein lipoylation reaction

The Journal of biological chemistry, 1995
Publisher Summary This chapter discusses the assay for protein lipoylation reaction. Lipoate attaches to the ɛ -amino group of the specific lysine residue of the proteins via an amide linkage. The lipoyllysine residue functions as a carrier of intermediates of the reactions and reducing equivalents between the active sites of the components of the ...
Kazuko Fujiwara, Kazuko Okamura-Ikeda, Yutaro Motokawa   +2 more
openaire   +3 more sources