Results 21 to 30 of about 618,514 (262)

The Mitogen-Activated Protein Kinase Slt2 Promotes Asymmetric Cell Cycle Arrest and Reduces TORC1-Sch9 Signaling in Yeast Lacking the Protein Phosphatase Ptc1

Microbiology Spectrum, 2023
Mitogen-activated protein kinase (MAPK) pathways regulate essential processes in eukaryotes. However, since uncontrolled activation of these cascades has deleterious effects, precise negative regulation of signaling flow through them, mainly executed by ...
Gema González-Rubio, Humberto Martín, María Molina   +2 more
doaj   +1 more source

Microcystins Alter Chemotactic Behavior in Caenorhabditis elegans by Selectively Targeting the AWA Sensory Neuron

Toxins, 2014
Harmful algal blooms expose humans and animals to microcystins (MCs) through contaminated drinking water. While hepatotoxicity following acute exposure to MCs is well documented, neurotoxicity after sub-lethal exposure is poorly understood. We developed
Caroline E. Moore, Pamela J. Lein, Birgit Puschner   +2 more
doaj   +1 more source

A dynamic charge-charge interaction modulates PP2A:B56 substrate recruitment

eLife, 2020
The recruitment of substrates by the ser/thr protein phosphatase 2A (PP2A) is poorly understood, limiting our understanding of PP2A-regulated signaling. Recently, the first PP2A:B56 consensus binding motif, LxxIxE, was identified. However, most validated
Xinru Wang, Dimitriya H Garvanska, Isha Nasa, Yumi Ueki, Gang Zhang, Arminja N Kettenbach, Wolfgang Peti, Jakob Nilsson, Rebecca Page   +8 more
doaj   +1 more source

A stable association with PME‐1 may be dispensable for PP2A demethylation – implications for the detection of PP2A methylation and immunoprecipitation

FEBS Open Bio, 2018
Reversible methyl‐esterification (methylation) of Leu309 in the protein phosphatase 2A catalytic subunit (PP2Ac) is essential for proper biogenesis of the PP2A holoenzyme.
Ryotaro Yabe, Shunya Tsuji, Satoru Mochida, Tsuyoshi Ikehara, Tatsuya Usui, Takashi Ohama, Koichi Sato   +6 more
doaj   +1 more source

Self-Association of Okadaic Acid: Structural and Pharmacological Significance

Marine Drugs, 2013
Okadaic acid (OA) has been an invaluable pharmacological tool in the study of cellular signaling. The great affinity of this polyether for its targets together with its high specificity to inhibit certain protein phosphatases enables the differential ...
José J. Fernández, Antonio Hernández Daranas, Alberto Hernández Creus, Patricia G. Cruz, Manuel Norte   +4 more
doaj   +1 more source

Oxidation Impacts the Intracellular Signaling Machinery in Hematological Disorders

Antioxidants, 2020
The dynamic coordination between kinases and phosphatases is crucial for cell homeostasis, in response to different stresses. The functional connection between oxidation and the intracellular signaling machinery still remains to be investigated.
Elena Tibaldi, Enrica Federti, Alessandro Matte, Iana Iatcenko, Anand B. Wilson, Veronica Riccardi, Mario Angelo Pagano, Lucia De Franceschi   +7 more
doaj   +1 more source

Protein Tyrosine Phosphatase Biochemical Inhibition Assays

Bio-Protocol, 2022
Disturbance of the dynamic balance between protein tyrosine phosphorylation and dephosphorylation, modulated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), is known to be crucial for the development of many human ...
Marek Baranowski, Jiaqian Wu, Ye Na Han, Lester Lambert, Nicholas Cosford, Lutz Tautz   +5 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Munc18-1 is a dynamically regulated PKC target during short-term enhancement of transmitter release

eLife, 2014
Transmitter release at synapses is regulated by preceding neuronal activity, which can give rise to short-term enhancement of release like post-tetanic potentiation (PTP).
Özgür Genç, Olexiy Kochubey, Ruud F Toonen, Matthijs Verhage, Ralf Schneggenburger   +4 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source