Central role of Tim17 in mitochondrial presequence protein translocation. [PDF]
Nature, 2023 Tim17 contains conserved negative charges close to the intermembrane space side of the bilayer, which are essential to initiate presequence protein translocation along a distinct transmembrane cavity of Tim17 for both classes of preproteins.
Fielden LF +17 more
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Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation. [PDF]
Int J Mol Sci, 2021 Most secreted and membrane proteins are targeted to and translocated across the endoplasmic reticulum (ER) membrane through the Sec61 protein-conducting channel.
Jung SJ, Kim H.
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Mechanism of Protein Translocation by the Sec61 Translocon Complex. [PDF]
Cold Spring Harb Perspect Biol, 2023 The endoplasmic reticulum (ER) is a major site for protein synthesis, folding, and maturation in eukaryotic cells, responsible for production of secretory proteins and most integral membrane proteins.
Itskanov S, Park E.
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Protein Translocation Control in E. coli via Temperature-Dependent Aggregation: Application to a Conditionally Lethal Enzyme, Levansucrase [PDF]
BiomoleculesPrecise control of protein translocation is essential for synthetic biology and protein engineering. Here, we present a temperature-responsive system using elastin-like polypeptides (ELPs) to regulate the translocation of a conditionally lethal enzyme in
Young Kee Chae
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How Quality Control Systems AID Sec-Dependent Protein Translocation [PDF]
Frontiers in Molecular Biosciences, 2021 The evolutionarily conserved Sec machinery is responsible for transporting proteins across the cytoplasmic membrane. Protein substrates of the Sec machinery must be in an unfolded conformation in order to be translocated across (or inserted into) the ...
Chen Jiang, Max Wynne, Damon Huber
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The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans [PDF]
mBio, 2023 The fungal pathogen Cryptococcus neoformans is distinguished by a cell-wall-anchored polysaccharide capsule that is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway.
Felipe H. Santiago-Tirado +6 more
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The SecA motor generates mechanical force during protein translocation. [PDF]
Nat Commun, 2020 The Sec translocon moves proteins across lipid bilayers in all cells. The Sec channel enables passage of unfolded proteins through the bacterial plasma membrane, driven by the cytosolic ATPase SecA.
Gupta R, Toptygin D, Kaiser CM.
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Clearing Traffic Jams During Protein Translocation Across Membranes [PDF]
Frontiers in Cell and Developmental Biology, 2021 Protein translocation across membranes is a critical facet of protein biogenesis in compartmentalized cells as proteins synthesized in the cytoplasm often need to traverse across lipid bilayers via proteinaceous channels to reach their final destinations.
Lihui Wang, Yihong Ye
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A functional link between the co-translational protein translocation pathway and the UPR
eLife, 2015 Upon endoplasmic reticulum (ER) stress, the transmembrane endoribonuclease Ire1α performs mRNA cleavage reactions to increase the ER folding capacity. It is unclear how the low abundant Ire1α efficiently finds and cleaves the majority of mRNAs at the ER ...
Rachel Plumb +3 more
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Voltage Sensing in Bacterial Protein Translocation. [PDF]
Biomolecules, 2020 The bacterial channel SecYEG efficiently translocates both hydrophobic and hydrophilic proteins across the plasma membrane. Translocating polypeptide chains may dislodge the plug, a half helix that blocks the permeation of small molecules, from its ...
Knyazev DG +5 more
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