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Protein Translocation Control in E. coli via Temperature-Dependent Aggregation: Application to a Conditionally Lethal Enzyme, Levansucrase [PDF]

Biomolecules
Precise control of protein translocation is essential for synthetic biology and protein engineering. Here, we present a temperature-responsive system using elastin-like polypeptides (ELPs) to regulate the translocation of a conditionally lethal enzyme in
Young Kee Chae
doaj   +2 more sources

How Quality Control Systems AID Sec-Dependent Protein Translocation [PDF]

Frontiers in Molecular Biosciences, 2021
The evolutionarily conserved Sec machinery is responsible for transporting proteins across the cytoplasmic membrane. Protein substrates of the Sec machinery must be in an unfolded conformation in order to be translocated across (or inserted into) the ...
Chen Jiang, Max Wynne, Damon Huber
doaj   +2 more sources

The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans [PDF]

mBio, 2023
The fungal pathogen Cryptococcus neoformans is distinguished by a cell-wall-anchored polysaccharide capsule that is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway.
Felipe H. Santiago-Tirado, Thomas Hurtaux, Jennifer Geddes-McAlister, Duy Nguyen, Volkhard Helms, Tamara L. Doering, Karin Römisch   +6 more
doaj   +2 more sources

Voltage Sensing in Bacterial Protein Translocation. [PDF]

Biomolecules, 2020
The bacterial channel SecYEG efficiently translocates both hydrophobic and hydrophilic proteins across the plasma membrane. Translocating polypeptide chains may dislodge the plug, a half helix that blocks the permeation of small molecules, from its ...
Knyazev DG, Kuttner R, Bondar AN, Zimmerman M, Siligan C, Pohl P.   +5 more
europepmc   +3 more sources

Clearing Traffic Jams During Protein Translocation Across Membranes [PDF]

Frontiers in Cell and Developmental Biology, 2021
Protein translocation across membranes is a critical facet of protein biogenesis in compartmentalized cells as proteins synthesized in the cytoplasm often need to traverse across lipid bilayers via proteinaceous channels to reach their final destinations.
Lihui Wang, Yihong Ye
doaj   +2 more sources

Signal Peptide Features Determining the Substrate Specificities of Targeting and Translocation Components in Human ER Protein Import

Frontiers in Physiology, 2022
In human cells, approximately 30% of all polypeptides enter the secretory pathway at the level of the endoplasmic reticulum (ER). This process involves cleavable amino-terminal signal peptides (SPs) or more or less amino-terminal transmembrane helices ...
Sven Lang, Duy Nguyen, Pratiti Bhadra, Martin Jung, Volkhard Helms, Richard Zimmermann   +5 more
doaj   +1 more source

Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis

eLife, 2021
The chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems.
Samuel James Watson, Na Li, Yiting Ye, Feijie Wu, Qihua Ling, R Paul Jarvis   +5 more
doaj   +1 more source

Reconstitution of surface lipoprotein translocation through the Slam translocon

eLife, 2022
Surface lipoproteins (SLPs) are peripherally attached to the outer leaflet of the outer membrane in many Gram-negative bacteria, playing significant roles in nutrient acquisition and immune evasion in the host.
Minh Sang Huynh, Yogesh Hooda, Yuzi Raina Li, Maciej Jagielnicki, Christine Chieh-Lin Lai, Trevor F Moraes   +5 more
doaj   +1 more source

Structural overview of the translocase of the mitochondrial outer membrane complex

Biophysics and Physicobiology, 2022
Most mitochondrial proteins are synthesized as precursor proteins (preproteins) in the cytosol and imported into mitochondria. The translocator of the outer membrane (TOM) complex functions as a main entry gate for the import of mitochondrial proteins ...
Yuhei Araiso, Toshiya Endo
doaj   +1 more source

Coordinated Translocation of Presequence-Containing Precursor Proteins Across Two Mitochondrial Membranes: Knowns and Unknowns of How TOM and TIM23 Complexes Cooperate With Each Other

Frontiers in Physiology, 2022
The vast majority of mitochondrial proteins are encoded in the nuclear genome and synthesized on cytosolic ribosomes as precursor proteins with specific mitochondrial targeting signals. Mitochondrial targeting signals are very diverse, however, about 70%
Marcel G. Genge, Dejana Mokranjac
doaj   +1 more source