Results 101 to 110 of about 734,035 (251)

Establishment of a High-Efficiency Protoplast Isolation and Transient Transformation System for Chrysanthemum Petals

Horticulturae
Chrysanthemum (Chrysanthemum × morifolium Ramat.) is a globally popular ornamental plant, but most cultivars lack efficient petal-based transient transformation systems, limiting floral trait molecular mechanism exploration.
Yanfei Li, Min Lu, Jiaying Wang, Chengyan Deng, Chenfei Lu, Yumeng Cui, Yuankai Tian, Boqia Feng, Yan Hong, Silan Dai   +9 more
doaj   +1 more source

Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase

Plant Biotechnology Journal, EarlyView.
ABSTRACT Breeding crops with robust disease resistance often compromises yield, a persistent challenge in agricultural improvement. We report that modifying cell wall architecture through a xylan‐specific glycosyltransferase successfully breaks this trade‐off in wheat.
Shufang Sun, Chunhui Li, Zehua Wang, Chunyang Ma, Xinhui Niu, Qunqing Wang, Qian Xu   +6 more
wiley   +1 more source

Isolation of Haustorium Protoplasts Optimized by Orthogonal Design for Transient Gene Expression in Phelipanche aegyptiaca

Plants
The efficient protoplast transient transformation system in plants is an important tool to study gene expression, metabolic pathways, and various mutagenic parameters, but it has not been established in Phelipanche aegyptiaca.
Xiaojian Zeng, Xiaolei Cao, Qiuyue Zhao, Siyuan Hou, Xin Hu, Zheyu Yang, Tingli Hao, Sifeng Zhao, Zhaoqun Yao   +8 more
doaj   +1 more source

Programmable Domestication: CRISPR, Pan‐Genomics and System Level Engineering for Next‐Generation Crops

Plant Biotechnology Journal, EarlyView.
Conceptual overview of programmable de novo domestication from genetic diversity discovery to crop deployment. The framework connects diverse germplasm and genomic resources with three trajectory pathways. Adaptive Rescue, Agronomic Refinement and Novel Chassis defined by biological distance, engineering complexity and breeding objectives.
Muhammad Mubashar Zafar, Hina Firdous, Ayesha Siddiqua, Ayesha Naveed, Abdul Razzaq, Sadam Munawar, Aqsa Ijaz, Zunaira Anwar, Sezai Ercisli, Xuefei Jiang, Qiao Fei   +10 more
wiley   +1 more source

Isolation and Transfection of Protoplasts From Maize Mesophyll Cells

Bio-Protocol
Protoplast systems are widely used in plant research as versatile platforms for studying cellular processes and validating gene editing tools. In maize, they are particularly valuable because stable transformation in immature embryos is slow and labor ...
Lauren Higa, Taren Bouwman, Zhi-Yan Du
doaj   +1 more source

First Report on Mesophyll Protoplast Isolation and Regeneration System for the Duboisia Species. [PDF]

Plants (Basel), 2023
Xue Y, Hiti-Bandaralage JCA, Hu Z, Zhao Z, Mitter N.   +4 more
europepmc   +1 more source

Protoplast isolation from Solanum lycopersicum L. leaf tissues and their response to short-term NaCl treatment [PDF]

, 2009
Protoplasts were isolated from young leaves of tomato (Solanum lycopersicon L. cvar Rio Fuego). The optimum conditions for protoplast isolation was established by using 2% cellulose R-10 and 0.5% macerozyme R-10 dissolved in 0.4 M sucrose-K3 solution for
Horváth Edit
core  

Protoplast isolation from Ulmusamericana L. pollen mother cells, tetrads, and microspores

, 1980
Meiotic protoplasts of Ulmusamericana (American elm) are potentially valuable for producing interspecific elm hybrids through protoplast fusion.
D. F. Karnosky, M. K. Redenbaugh, R. D. Westfall   +2 more
core   +1 more source

Single‐Cell Transcriptomics Reveals the FA9–VAP Module Regulating Fatty Acid Accumulation in Soybean Seeds

Plant Biotechnology Journal, EarlyView.
ABSTRACT As a major commercial legume crop, soybean ranks among the world's most significant sources of edible oil and plant protein. We previously identified a SEIPIN homologue (FA9) at the fatty acid 9 locus that promotes fatty acid accumulation in soybean.
Hui Li, Tianshu Li, Xiaorui Xu, Jinhang Cui, Xin Chen, Jingyi Huang, Jia Song, Cui Mu, Xinyu Hong, Chunyan Liu, Ning Wang, Xue Han, Sui Wang, Qingshan Chen, Zhaoming Qi   +14 more
wiley   +1 more source

Factors affecting mango (Mangifera indica L.) protoplast isolation and culture

, 2011
The major factors influencing protoplast isolation and culture of mango (Mangifera indica L.) cv. Kensington Pride were investigated. The resultant protocol was used to compare plating efficiency among 4 mango cultivars.
Richard R. Williams, Ramezan Rezazadeh, Rezazadeh, Ramezan, Dion K. Harrison, Harrison, Dion K., Williams, Richard R.   +5 more
core   +1 more source