Results 101 to 110 of about 698,061 (253)

A review: protoplast isolation by using different explant / Nurul Huda Mustafa [PDF]

, 2016
This review study was conducted on isolation of protoplast using A review: protoplast isolation by using different explant A review: protoplast isolation by using different explant and was focusing on different explants of major crops.
Mustafa, Nurul Huda
core  

Establishment of a High-Efficiency Protoplast Isolation and Transient Transformation System for Chrysanthemum Petals

Horticulturae
Chrysanthemum (Chrysanthemum × morifolium Ramat.) is a globally popular ornamental plant, but most cultivars lack efficient petal-based transient transformation systems, limiting floral trait molecular mechanism exploration.
Yanfei Li, Min Lu, Jiaying Wang, Chengyan Deng, Chenfei Lu, Yumeng Cui, Yuankai Tian, Boqia Feng, Yan Hong, Silan Dai   +9 more
doaj   +1 more source

Targeted Modulation of Eukaryotic Release Factor 1 (OseRF1) by an RNA Aptamer Enhances Drought Adaptation in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Drought stress severely restricts rice productivity, necessitating strategies to enhance stress resilience. Here, we identify translation termination as a tunable regulatory node for stress adaptation. Using 25 rounds of SELEX, we isolated RTAR, an RNA aptamer that binds the rice eukaryotic release factor 1 (OseRF1) with nanomolar affinity ...
Shuangfeng Dai, Mingming Chen, Haomin Chen, Yuantao Ye, Hong Huang, Renrong Zhang, Zhenyu Guo, Yongxiang Huang   +7 more
wiley   +1 more source

Isolation of Haustorium Protoplasts Optimized by Orthogonal Design for Transient Gene Expression in Phelipanche aegyptiaca

Plants
The efficient protoplast transient transformation system in plants is an important tool to study gene expression, metabolic pathways, and various mutagenic parameters, but it has not been established in Phelipanche aegyptiaca.
Xiaojian Zeng, Xiaolei Cao, Qiuyue Zhao, Siyuan Hou, Xin Hu, Zheyu Yang, Tingli Hao, Sifeng Zhao, Zhaoqun Yao   +8 more
doaj   +1 more source

First Report on Mesophyll Protoplast Isolation and Regeneration System for the Duboisia Species. [PDF]

Plants (Basel), 2023
Xue Y, Hiti-Bandaralage JCA, Hu Z, Zhao Z, Mitter N.   +4 more
europepmc   +1 more source

The E3 Ubiquitin Ligases SINA3 and SINA5 Control DEP2 Ubiquitination and Proteasomal Degradation to Regulate Grain Size and Weight in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Grain size is a critical agronomic trait, yet the molecular mechanisms governing its determination in crops remain incompletely understood. While recent studies revealed that OsRING80 facilitates DENSE AND ERECT PANICLE 2 (DEP2) degradation to mediate immunity without impacting growth and development, our work identifies a distinct regulatory ...
Hongming Wu, Xiejun Sun, Xin Wang, Chen Xie, Yingli Lv, Xiaoman You, Yu Zhang, Xiaohang Han, Yun Zhu, Yimin Lin, Yehui Xiong, Qibing Lin, Cailin Lei, Xiuping Guo, Shanshan Zhu, Zhijun Cheng, Yulong Ren, Ling Jiang, Jianmin Wan   +18 more
wiley   +1 more source

Isolation of Pichia manshurica protoplast from Dahlia sp plant

, 2014
Isolation of protoplasts is an important step in the fusion process. Protoplasts are cells that have eliminated the cell wall, but the cell membranes and organs can still function properly.
Kumala Dewi, Wijanarka Wijanarka, Endang Sutariningsih Soetarto, Ari Indrianto   +3 more
core   +1 more source

Penicillin-binding proteins of protoplast and sporoplast membranes of Streptomyces griseus strains [PDF]

, 1988
Membrane-bound penicillin-binding proteins (PBPs) of two Streptomyces griseus strains that sporulate well in liquid and solid medium have been investigated during the course of their life-cycle.
Szabó, István,, Harder, Wim,, Harder, Willem, Veenhuis, Marten,, Barabás, György,, Barabás, Judit,, Veenhuis, Marten, Barabás, Judit, Barabás, György, Szabó, István   +9 more
core   +1 more source

Isolation and Transfection of Protoplasts From Maize Mesophyll Cells

Bio-Protocol
Protoplast systems are widely used in plant research as versatile platforms for studying cellular processes and validating gene editing tools. In maize, they are particularly valuable because stable transformation in immature embryos is slow and labor ...
Lauren Higa, Taren Bouwman, Zhi-Yan Du
doaj   +1 more source

Single‐Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum

Plant Biotechnology Journal, EarlyView.
ABSTRACT B chromosomes are supernumerary entities found in many plant species, with some exhibiting tissue‐specific elimination. In Sorghum purpureosericeum, extensive B chromosome elimination occurs during embryogenesis affecting most of the embryonic organs, leaving the B chromosome maintained mainly in limited regions of meristems.
T. Bojdová, P. Abaffy, L. Valihrach, M. Karafiátová, J. Bartoš   +4 more
wiley   +1 more source