Results 121 to 130 of about 21,306 (282)

トマト サイバイシュ L.esculentum ノ シヨウ ノ ヨウニク プロトプラスト ノ サイボウ ブンレツ ト コロニー ケイセイ ニ オヨボス マエ ショリ ノ エイキョウ [PDF]

, 1993
【摘要】トマト栽培種の葉肉プロトプラスト培養の効率化を図る目的で，栽培種の“強力東光を主な供試材料として，その幼苗の子葉にプロトプラストを単離する前に行う処理(以下，前処理と称する)が子葉プロトプラストの収量，分裂率およびコロニー形成率に及ぼす影響について検討した.また，得られたプロトプラストの培養における培地組成や植物ホルモンの添加および培養方法の影響についても調査した.その結果，前処理の影響は処理液と培養培地によって異なることが分かった.すなわち ...
今西 茂, 樋浦 巌, 陳 蘭荘
core   +1 more source

A Truncated WRKY Protein Enhances Drought Resistance in Wild Tomatoes Through the SlWRKY16‐CIP2b‐SlSYP121 Module

Plant Biotechnology Journal, EarlyView.
ABSTRACT Drought stress is a major abiotic factor that severely affects plant growth and food production. Identifying drought‐resistant genes and their regulatory mechanisms is essential for mitigating the negative impacts of drought on plants. In this study, we identified a natural single nucleotide polymorphism (SNP) mutation in SlWRKY16 that is ...
Yin Ding, Xuting Chen, Ke Wu, Huifang Hou, Yaling Wang, Bentao Yan, Muhammad Hafeez Ul Rehman Khursheed, Chunyu Shang, Xingguo Zhang, Yu Pan, Lang Wu, Jinhua Li   +11 more
wiley   +1 more source

AhBWR15, A Novel RLK Gene, Confers Resistance to Ralstonia solanacearum in Peanut

Plant Biotechnology Journal, EarlyView.
ABSTRACT Bacterial wilt (BW), a severe soil‐borne disease caused by Ralstonia solanacearum, significantly impedes global peanut production. Despite its impact, the mechanisms underlying BW resistance in peanut remain unclear. Herein, we selected the highly resistant variety Nongdahua108 (H108) and the susceptible variety Nongdahua107 (H107) to develop ...
Zenghui Cao, Kai Zhao, Hao Li, Xingli Ma, Di Cao, Yanzhe Li, Sasa Hu, Qian Ma, Yaoyao Li, Yi Fan, Mengtian Hou, Kunkun Zhao, Ding Qiu, Fangping Gong, Zhongfeng Li, Rui Ren, Dongmei Yin   +16 more
wiley   +1 more source

A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Fragaria × ananassa)

Plants
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a simple and powerful tool, which enables gene knockout or insertion of new gene cassettes. This method has been applied to various plants and
Yunsun Kim, Jihye Jang, Youngjae Oh, Seonghee Lee, Beum-Chang Kang   +4 more
doaj   +1 more source

A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture. [PDF]

Micromachines (Basel), 2022
Zhang H, Geng Q, Sun Z, Zhong X, Yang Y, Zhang S, Li Y, Zhang Y, Sun L.   +8 more
europepmc   +1 more source

Multi‐Omics Insights Into Anthraquinone Biosynthesis in Rheum tanguticum

Plant Biotechnology Journal, EarlyView.
ABSTRACT Rheum tanguticum is renowned for its medicinal properties, including purgative, anti‐inflammatory and hepatoprotective effects, primarily attributed to anthraquinones (AQs). However, the molecular mechanisms of AQs biosynthesis have largely been hindered by insufficient genomic resources and functional genomics investigations.
Shuo Zhao, Yuan Zong, Tao Wang, Jianan Li, Zengrong Ye, Famei Zhang, Guoying Zhou   +6 more
wiley   +1 more source

PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS) [PDF]

Journal of Sciences, Islamic Republic of Iran, 2001
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g.
doaj  

The Rice Cis‐Natural Antisense Transcript NAT1850 of Pri‐miR1850 Negatively Regulates Cold Tolerance by Repressing NPR3

Plant Biotechnology Journal, EarlyView.
ABSTRACT Natural antisense transcripts (NATs) correspond to nearly 60% of annotated rice loci, however their functions are largely unknown. In this study, we characterise a rice cis‐NAT (NAT1850) that completely overlaps with a rice‐specific primary miRNA, pri‐miR1850.
Yang Shen, Yan Wang, Lijia Yang, Dongpeng Li, Weifeng Dong, Xijuan Zhang, Weibing Yang, Hui Xie, Jin Liu, Shiyong Song, Mingzhe Sun, Xiaoli Sun   +11 more
wiley   +1 more source

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis. [PDF]

Plant Methods, 2023
Zhang K, Liu S, Fu Y, Wang Z, Yang X, Li W, Zhang C, Zhang D, Li J.   +8 more
europepmc   +1 more source

Natural Variations in the Activity of Hydroxycinnamoyl Transferases Promote Accumulation of Metabolites Conferring Rice Resistance

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant‐derived secondary metabolites act as regulators of in planta defence. Plant phenylpropanoid metabolic compounds, including final metabolites such as lignin and flavonoids, and a few intermediate metabolites play critical roles in defence.
Dan Chen, Jiyun Liu, Guixin Yuan, Yingshuang Liu, Wenqian Zhu, Ying Chen, Junyou Geng, Jing Fan, Xiaojing Dong, Meng Yuan   +9 more
wiley   +1 more source