Results 121 to 130 of about 698,061 (253)

PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR DELAWARE USING SILVER THIOSULFATE (STS) [PDF]

Journal of Sciences, Islamic Republic of Iran, 2001
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g.
doaj  

A Stable and Rapid Protoplast-Based CRISPR/Cas9-Ribonucleoprotein Complex Screening System for Octoploid Strawberry (Fragaria × ananassa)

Plants
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a simple and powerful tool, which enables gene knockout or insertion of new gene cassettes. This method has been applied to various plants and
Yunsun Kim, Jihye Jang, Youngjae Oh, Seonghee Lee, Beum-Chang Kang   +4 more
doaj   +1 more source

A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture. [PDF]

Micromachines (Basel), 2022
Zhang H, Geng Q, Sun Z, Zhong X, Yang Y, Zhang S, Li Y, Zhang Y, Sun L.   +8 more
europepmc   +1 more source

SbWRKY62 Coordinates With SbERF096 to Activate Pterostilbene Biosynthesis and Targets a Xylanase Inhibitor Protein for Anthracnose Resistance in Sorghum

Plant, Cell &Environment, EarlyView.
ABSTRACT Sorghum anthracnose caused by Colletotrichum sublineola (C. sublineola) is a destructive disease leading to severe crop damages. Pterostilbene is a phytoalexin that contributes to defence responses in sorghum, but the transcriptional regulation of its biosynthesis remained poorly understood.
Yajing Li, Bofu Deng, Yiming Gan, Nan Lin, Dan Zhou, Clive Lo   +5 more
wiley   +1 more source

Rice Orange Leaf Phytoplasma Imp Protein Blocks G–M Interaction by Binding Glycoprotein G to Suppress Rice Stripe Mosaic Virus Infection

Plant, Cell &Environment, EarlyView.
ABSTRACT Plants frequently encounter multiple pathogens concurrently, yet the molecular bases of pathogen–pathogen interactions during mixed infections remain largely unresolved. Here we show that co‐infection of rice by Rice orange leaf phytoplasma (ROLP) and Rice stripe mosaic virus (RSMV) markedly suppresses RSMV proliferation in both host plants ...
Jiaxin Qiu, Yujiao Zheng, Yanmei Yi, Yuxin Zhang, Tianbao Zeng, Hongyan Chen, Taiyun Wei, Xiao‐Feng Zhang   +7 more
wiley   +1 more source

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis. [PDF]

Plant Methods, 2023
Zhang K, Liu S, Fu Y, Wang Z, Yang X, Li W, Zhang C, Zhang D, Li J.   +8 more
europepmc   +1 more source

Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15‐mediated unloading of sucrose from the host's phloem

New Phytologist, Volume 252, Issue 2, Page 829-842, October 2026.
Cuscuta reflexa infection sites. Summary Cuscuta species are holoparasitic plants that penetrate host stems with haustoria, connect to the vasculature and exhaust water, nutrients and carbohydrates. Parasite seedlings possess only limited maternal reserves and the development of a vascular connection takes several days.
Maleen Hartenstein, Dominik Eydam, Antonia Klatt, Arne Greif, Peter Slaby, Ronja Burggraf, Franz Klebl, Isabell Albert, Uwe Sonnewald, Ruth Stadler, Markus Albert   +10 more
wiley   +1 more source

A highly efficient protocol for tomato protoplast isolation and transfection via seedling etiolation

Applied Biological Chemistry
Protoplast-based transient expression systems serve as indispensable platforms for functional genomics, genome editing, and synthetic biology. While tomato (Solanum lycopersicum) is a premier model crop for genetic research, its utility in protoplast ...
Yaerim Lee, So Hee Yang, Soon-Ki Han, Yeonjong Koo   +3 more
doaj   +1 more source

Mutual antagonism between auxin and diacylglycerol acyltransferase 2 regulates high‐temperature‐induced hypocotyl elongation

New Phytologist, Volume 252, Issue 1, Page 222-240, October 2026.
Proposed model for auxin–DGAT2 crosstalk regulating high‐temperature‐induced hypocotyl elongation. Summary Cell elongation is a fundamental process in plant growth and development, and it is significantly affected by temperature changes. While the signaling mechanisms driving high‐temperature‐induced cell elongation have been extensively studied, the ...
Mohinur Khamzaaliyeva, Punita Lalchand, Didier‐Deschamps Ashley, Xue Pan   +3 more
wiley   +1 more source

Optimization of Establishment, Protoplast Separation, and Fusion via Embryonic Suspension System in Chestnut (Castanea mollissima Bl.)

Agronomy
Castanea mollissima Bl. is rich in nutrition and strong in stress resistance, and has nutritional, economic, and ecological values. A protoplast is impactful in somatic fusion and germplasm creation.
Shiying Zhang, Sujuan Guo, Ruijie Zheng
doaj   +1 more source