Results 131 to 140 of about 698,061 (253)

Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization

Plant, Cell &Environment, Volume 49, Issue 10, Page 7449-7469, October 2026.
ABSTRACT Global warming impacts several aspects of plant physiology, with important negative effects on crop yield and production of secondary metabolites, such as anthocyanins. The anthocyanin content of vegetables and fruits has attracted public interest in the last two decades due to its health benefits, leading to the development of novel ...
Jacopo Menconi, Noemi La Monaca, Irene Cataldo, Pietro Maria Niccolini, Pierdomenico Perata, Silvia Gonzali   +5 more
wiley   +1 more source

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, Volume 13, Issue 49, 3 September 2026.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

Chromosome variation in protoplast-derived potato plants

, 1982
Chromosomes have been studied in protoplast-derived potato plants of the tetraploid cultivars Maris Bard and Fortyfold. A high degree of aneuploidy was found amongst the regenerants of both cultivars but the nature of the chromosome variation differed ...
Nelson, R. S., Bright, S. W. J., Karp, A., Thomas, E.   +3 more
core   +1 more source

PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry

Plant Methods
Background Black huckleberry (Vaccinium membranaceum) is a native fruit species of high nutritional, medicinal, ecological, and economic value. The black huckleberries, abundant in bioactive compounds, offer significant antioxidants and anti-inflammatory
Sweety Majumder, Abir U. Igamberdiev, Samir C. Debnath   +2 more
doaj   +1 more source

An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.
Xiaoxiao Yu, Jintai Yu, Yin Lu, Wenjing Li, Guanzhong Huo, Jun Zhang, Yan Li, Jianjun Zhao, Jun Li   +8 more
doaj   +1 more source

Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2. [PDF]

Int J Mol Sci, 2022
Biswas S, Wahl NJ, Thomson MJ, Cason JM, McCutchen BF, Septiningsih EM.   +5 more
europepmc   +1 more source

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS‐PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR/Cas9 editing

Pest Management Science, Volume 82, Issue 9, Page 8189-8198, September 2026.
A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos, Athanasios Petmezas, Anastasios Samaras, George Karaoglanidis   +3 more
wiley   +1 more source

Nitrogen‐Starved Wheat: A Multi‐Omics Perspective From Epigenome Regulation to Grain Quality

Journal of Sustainable Agriculture and Environment, Volume 5, Issue 3, September 2026.
This review establishes epigenetic regulation, specifically H3K27ac and H3K27me3 dynamics at nitrogen transporter, assimilation, and storage protein loci, as a principal determinant of cultivar‐specific nitrogen use efficiency in hexaploid wheat, independent of DNA sequence variation, and identifies the rhizosphere microbiome as an integral but ...
Baber Ali, Zeeshan Khan, Nijat Imin
wiley   +1 more source

Protoplast isolation from Solanum lycopersicum L. leaf tissues and their response to short-term NaCl treatment [PDF]

, 2009
Protoplasts were isolated from young leaves of tomato (Solanum lycopersicon L. cvar Rio Fuego). The optimum conditions for protoplast isolation was established by using 2% cellulose R-10 and 0.5% macerozyme R-10 dissolved in 0.4 M sucrose-K3 solution for
Horváth Edit
core  

An Improved Approach to Protoplast Regeneration and Transfection in Banana (Musa acuminata AAA cv. Williams)

Applied Biosciences
Protoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo, Jean-Yves Paul, Anthony James, Rob Harding, James Dale   +4 more
doaj   +1 more source