Results 141 to 150 of about 698,061 (253)

Optimization of Protoplast Isolation from Leaf Mesophylls of Chinese Cabbage (Brassica rapa ssp. pekinensis) and Subsequent Transfection with a Binary Vector. [PDF]

Plants (Basel), 2021
Sivanandhan G, Bae S, Sung C, Choi SR, Lee GJ, Lim YP.   +5 more
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3196-3207, September 2026.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Additional file 1: of Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

, 2016
Selection of appropriate Phaseolus vulgaris tissue material for protoplast isolation. (A) Wing and keel petals were excised, retaining the pink-colored portions, for protoplast isolation. (B) The roots of 3-day-old germinated seeds were cut 3 mm from tip
Manoj-Kumar Arthikala (3587666), Miguel Lara (320017), Elizabeth Arellano (3587669), Lourdes Blanco (3587672), Kalpana Nanjareddy (3587663)   +4 more
core   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3316-3328, September 2026.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3231-3242, September 2026.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3458-3472, September 2026.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Engineering glyphosate‐resistant watermelon by prime editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3298-3300, September 2026.
Editing the watermelon EPSPS gene using a prime editing platform with visible markers created a non‐transgenic glyphosate‐resistant line. These robust heterozygous mutants tolerate field‐level herbicide doses without growth penalties, serving as ideal parental lines for crop breeding.
Shouwei Tian, Yu Lu, Jingchao Chen, Guoyi Gong, Haiying Zhang, Jie Zhang, Changlong Wen, Yong Xu   +7 more
wiley   +1 more source

The ER–Golgi Lipid Exchanger PsORP1 Is Essential for Phytophthora sojae Development and Is Blocked by Oxathiapiprolin via Its OSBP‐Related Domain (ORD)

Molecular Plant Pathology, Volume 27, Issue 9, September 2026.
PsORP1 anchors ER–Golgi MCSs to mediate PS/PI4P counter‐transport, and oxathiapiprolin targets the ORD to inhibit this lipid exchange. ABSTRACT Oxysterol‐binding protein (OSBP)‐related proteins (ORPs) are indispensable eukaryotic lipid transporters and promising targets for pharmaceutical and agrochemical exploitation.
Xiaofei Liu, Chengcheng Li, Guangda Shao, Qin Peng, Haixia Wang, Jianqiang Miao, Xili Liu   +6 more
wiley   +1 more source

The Conserved Guanyl‐Specific Ribonuclease T1 Effector EC19 Is Required for Full Virulence of the Vascular Wilt Fungus Fusarium oxysporum on Tomato

Molecular Plant Pathology, Volume 27, Issue 9, September 2026.
EC19, a secreted ribonuclease T1 effector from Fusarium oxysporum f. sp. lycopersici (Fol), is required for tomato wilt virulence. EC19‐overexpressing tomato lines display increased susceptibility to Fol and altered transcriptional changes associated with plant hormone signal transduction, plant–pathogen interaction, and phenylpropanoid biosynthesis ...
Min Li, Yifei Wang, Haoqian Li, Xizhe Sun, Jianbiao Wang, Xiangling Fang, Jianjun Zhao, Lisong Ma   +7 more
wiley   +1 more source

Systematic Identification and Functional Characterisation of Colletotrichum fructicola Effectors During Camellia oleifera Colonisation

Molecular Plant Pathology, Volume 27, Issue 9, September 2026.
Five novel effectors were identified in Colletotrichum fructicola required for full virulence. They suppress host immunity, modulate antioxidant enzymes and reprogramme metabolism in Camellia oleifera, providing targets for anthracnose control. ABSTRACT Camellia oleifera is an important woody oil crop in southern China, but its production is severely ...
Lingxue Cao, Wentong Dong, Kaile Sun, Xizhe Sun, Chenxue Song, Lisong Ma, Geng Meng   +6 more
wiley   +1 more source