Results 91 to 100 of about 393,999 (153)

PROTOPLAST FORMATION AND REGENERATION FROM SPORANGIA AND ENCYSTED ZOOSPORES OF PHYTOPHTHORA-INFESTANS

, 2016
Protoplasts of Phytophthora infestans were formed from sporangia or encysted zoospores by digestion with NovoZym 234 in stabilizing osmotica. The highest yields, representing a conversion of up to 90% of initial sporangia or cysts were obtained in solKCl

core   +1 more source

DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

Frontiers in Genome Editing
Protoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes.
Ryan Creeth, Andrew Thompson, Zoltan Kevei   +2 more
doaj   +1 more source

Genome Editing and Protoplast Regeneration to Study Plant-Pathogen Interactions in the Model Plant Nicotiana benthamiana. [PDF]

Front Genome Ed, 2020
Hsu CT, Lee WC, Cheng YJ, Yuan YH, Wu FH, Lin CS.   +5 more
europepmc   +1 more source

Improvement of astaxanthin production from Phaffia rhodozyma by protoplast fusion

, 2003
Protoplast fusion between intrastrain of P. rhodozyma has been generated in order to increase astaxanthin production. The best protoplast was obtained under osmotic stabilizer using buffer phosphate with the addition of 0.1 M CaC12.
Perpustakaan UGM, i-lib
core   +1 more source

Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing

Applied Biological Chemistry
The Solanaceae family includes the largest flowering crops such as tomatoes, potatoes, and eggplants. Consumer demand has led to massive development of plants in the Solanum genus, and many different Solanum varieties are now available on the market. The
So Hee Yang, Suk Weon Kim, Sujin Lee, Yeonjong Koo   +3 more
doaj   +1 more source

Establishment of Protoplast Culture of Solanum sisymbriifolium

, 2006
The optimal calture conditions were studied for plant regeneration from mesophyll protoplasts of Solanum sisymbriidorium. Aeseptic seedlings of S.sisymbriidorium were used as a meterial for protoplast culture.
Isshiki, Shiro, Sadohara, Takeshi, Okubo, Hiroshi, Oda, Naoki, 一色, 司郎, 大久保, 敬, 尾崎, 行生, Ozaki, Yukio   +7 more
core  

PROTOPLAST-BASED TECHNOLOGY FOR IMPLEMENTATION OF NEW BREEDING TECHNIQUES IN CHICORY spp. [PDF]

openChicory (Cichorium intybus L.) is a nutritionally and medicinally valuable plant with increasing significance in modern agriculture and biotechnology. This study aimed to optimize in vitro culture, protoplast isolation, and transfection protocols for
MUSARRAT, HADEENA
core  

Factors affecting protoplast isolation and cultivation of Cucumis spp.

Journal of Applied Botany and Food Quality, 2012
Protoplasts of Cucumis anguria, Cucumis melo (3 accessions), Cucumis metuliferus and Cucumis sativus were isolated from leaves, growing apices, hypocotyls and calluses. Plants were cultured on 2 concentrations of sucrose.
J. Gajdová, B. Navrátilová, J. Smolná, A. Lebeda   +3 more
doaj  

Comprehensive transcriptomic and functional characterization of protoplast regeneration in Angelica gigas Nakai. [PDF]

BMC Plant Biol
Lee HS, Han JE, Shim D, Choi BY, Cho H, Murthy HN, Park SY.   +6 more
europepmc   +1 more source

Correction: Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing of <i>Brassica carinata</i>. [PDF]

Front Plant Sci
Frontiers Production Office .
europepmc   +1 more source