Results 81 to 90 of about 393,999 (153)

Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars.

, 1986
The regeneration of protoplasts from potato (Solanum tuberosum L.) cvs. Desiree and King Edward has been significantly improved. Different shoot culture media were required for the release of viable protoplasts from cvs.
Jones, M.G.K., Jones, M. G. K., Foulger, D.   +2 more
core  

Barley scutellum protoplasts: isolation, culture and plant regeneration

, 1996
Many applications of cereal protoplast culture systems are still limited by the difficulties of regeneration from suspension cells which are the usual protoplast source.
Davey, M. R., Nobre, J., Lazzeri, P. A.
core   +1 more source

Lactobacillus protoplast transformation. Plasmid 17, 73-75.

, 1987
A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake.
MORELLI L, COCCONCELLI PS, SGARAMELLA V., DAMIANI G, FERRETTI, LUCA, BOTTAZZI V   +5 more
core   +1 more source

Corrigendum: Efficient protoplast regeneration protocol and CRISPR/Cas9-mediated editing of glucosinolate transporter (GTR) genes in rapeseed (Brassica napus L.). [PDF]

Front Plant Sci, 2023
Li X, Sandgrind S, Moss O, Guan R, Ivarson E, Wang ES, Kanagarajan S, Zhu LH.   +7 more
europepmc   +1 more source

5. Somatic cell genetics: Manipulating plants through single-cell techniques

California Agriculture, 1982
Protoplast regeneration/Engler, Grogan; Regeneration of plants/Murashige; Somaclonal variation/Orton; Cell mutagens/G.
Z Sung, I Furner
doaj  

Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration. [PDF]

CRISPR J, 2021
Hsu CT, Yuan YH, Lin YC, Lin S, Cheng QW, Wu FH, Sheen J, Shih MC, Lin CS.   +8 more
europepmc   +1 more source

Enzymatic Preparation and Regeneration of Protoplasts from the Red Microalga Porphyridium purpureum

Oceans
Gene editing techniques are well-developed for model microalgae like Chlorella and Chlamydomonas. However, for the high-value species Porphyridium purpureum, its thick cell wall poses a significant barrier to genetic manipulation.
Bingqi Xu, Yiyang Wu, Wenzhou Xiang, Yaqi Geng, Liang Wei, Lingyu Ouyang, Houbo Wu, Hualian Wu, Jin Xu, Tao Li   +9 more
doaj   +1 more source

DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration. [PDF]

Plant Physiol, 2022
Lin CS, Hsu CT, Yuan YH, Zheng PX, Wu FH, Cheng QW, Wu YL, Wu TL, Lin S, Yue JJ, Cheng YH, Lin SI, Shih MC, Sheen J, Lin YC.   +14 more
europepmc   +1 more source

Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants

Plants
Advances in cell fusion technology have propelled breeding into the realm of somatic hybridization, enabling the transfer of genetic material independent of sexual reproduction.
Shuping Liu, Xiaojie Li, Jiani Zhu, Yihong Jin, Chuizheng Xia, Bingsong Zheng, Cristian Silvestri, Fuqiang Cui   +7 more
doaj   +1 more source

Protoplast preparation and reversion to the normal filamentous growth in antibiotic-producing uncommon actinomycetes.

, 2010
Protoplast preparation, regeneration and fusion represent essential tools for those poorly studied biotechnologically valuable microorganisms inapplicable with the current molecular biology protocols.
L. Carrano, Flavia Marinelli, F. Beltrametti, Lucia Carrano, MARINELLI, FLAVIA, Giorgia Letizia Marcone, Fabrizio Beltrametti, MARCONE, GIORGIA LETIZIA   +7 more
core   +1 more source