Physcomitrium patens Protoplasting and Protoplast Transfection
, 2022 International audienceProtoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base of reverse genetic studies covering research fields as diverse as development ...
Perroud, Pierre-François +7 more
core +1 more source
Applied BiosciencesProtoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo +4 more
doaj +1 more source
Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314
, 2011 94-96Method for production and regeneration of Acetobacter pasteurianum NCIM 2314 protoplasts are described. The protoplasts were obtained by treatment with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers.
Gokhale, D V +2 more
core
Genetic variability in regenerated Metarhizium flavoviride protoplasts
Brazilian Archives of Biology and Technology, 2004 Protoplast isolation and regeneration were evaluated in two wild-type and two colour mutant strains of Metarhizium flavoviride. Cultivation in liquid medium, followed by mycelium treatment with Novozym 234 in the presence of KCl 0.7M as osmotic ...
Júlia Kuklinsky-Sobral +3 more
doaj +1 more source
Corrigendum: Establishment of an efficient protoplast regeneration and transfection protocol for field cress (Lepidium campestre). [PDF]
Front Genome Ed, 2023 Sandgrind S +4 more
europepmc +1 more source
Genetic improvement of Oenological Interesting Lucanian Grapevine Varieties through CRISPR/Cas9-Mediated Mutagenesis: Somatic embryogenesis and protoplast regeneration [PDF]
BIO Web of ConferencesCultivated grapevine varieties, propagated vegetatively, exhibit intra-varietal genetic variability due to somatic mutations. While this variability has been exploited in the past for clonal selection, current environmental challenges and market demands ...
Giorio Giovanni +4 more
doaj +1 more source
Stochastic gene expression drives mesophyll protoplast regeneration. [PDF]
Sci Adv, 2021 Xu M, Du Q, Tian C, Wang Y, Jiao Y.
europepmc +1 more source
DNA-free genome editing in grapevine using CRISPR/Cas9 ribonucleoprotein complexes followed by protoplast regeneration. [PDF]
Hortic Res, 2023 Najafi S +4 more
europepmc +1 more source
Guangdong nongye kexue【Objective】Cordyceps guangdongensis, a rare edible-medicinal fungus in China, has been approved as a new resource food, with a broad prospect of industrial application.
Chenghua ZHANG +7 more
doaj +1 more source
Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum). [PDF]
Plant Cell Rep, 2022 Liu Y +5 more
europepmc +1 more source