Nitro Reduction‐Based RNA Control and Ultrafast Release
Angewandte Chemie, EarlyView.A chemical RNA caging–uncaging strategy based on nitro reduction is described. Nitroaryl groups introduced via acylation temporarily block the functions of diverse RNAs in vitro and in living cells. Treatment with a diboronic acid–bipyridine mixture rapidly reduces the nitro groups, releasing RNAs and restoring their activity.
Yiran Zhao +6 more
wiley +2 more sources
Construction and Immunogenicity of Modified mRNA-Vaccine Variants Encoding Influenza Virus Antigens
Vaccines, 2021 Nucleic acid-based influenza vaccines are a promising platform that have recently and rapidly developed. We previously demonstrated the immunogenicity of DNA vaccines encoding artificial immunogens AgH1, AgH3, and AgM2, which contained conserved ...
Ekaterina V. Starostina +14 more
doaj +1 more source
Spliceosomal snRNA Epitranscriptomics
Frontiers in Genetics, 2021 Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell ...
Pedro Morais, Hironori Adachi, Yi-Tao Yu
doaj +1 more source
Recent Advances in Identification of RNA Modifications
Non-Coding RNA, 2016 RNA modifications are involved in a broad spectrum of biological and physiological processes. To reveal the functions of RNA modifications, it is important to accurately predict their positions.
Wei Chen, Hao Lin
doaj +1 more source
Pseudouridines of tRNA Anticodon Stem-Loop Have Unexpected Role in Mutagenesis in Pseudomonas sp.
Microorganisms, 2020 Pseudouridines are known to be important for optimal translation. In this study we demonstrate an unexpected link between pseudouridylation of tRNA and mutation frequency in Pseudomonas species.
Mari Tagel +5 more
doaj +1 more source
H/ACA Small Ribonucleoproteins: Structural and Functional Comparison Between Archaea and Eukaryotes
Frontiers in Microbiology, 2021 During ribosome synthesis, ribosomal RNA is modified through the formation of many pseudouridines and methylations which contribute to ribosome function across all domains of life.
Dominic P. Czekay, Ute Kothe
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Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress
Antibiotics, 2023 Bacterial ribosome small subunit rRNA (16S rRNA) contains 11 nucleotide modifications scattered throughout all its domains. The 16S rRNA pseudouridylation enzyme, RsuA, which modifies U516, is a survival protein essential for bacterial survival under ...
Sudeshi M. Abedeera +4 more
doaj +1 more source
Pseudouridine synthases are proviral factors for Sindbis virus in insect and mammalian cells
mBioAlphaviruses are positive-sense, single-stranded RNA (+ssRNA) viruses transmitted by arthropod vectors to vertebrate hosts. Pseudouridine is the most prevalent RNA modification in the prototype alphavirus, Sindbis virus (SINV) genome, but the location ...
Nicole Stark +6 more
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Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA
Microorganisms, 2018 To date, numerous modified nucleosides in tRNA as well as tRNA modification enzymes have been identified not only in thermophiles but also in mesophiles.
Hiroyuki Hori +6 more
doaj +1 more source
Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae
Biomolecules, 2020 Pus1-dependent pseudouridylation occurs in many tRNAs and at multiple positions, yet the functional impact of this modification is incompletely understood.
Bahar Khonsari, Roland Klassen
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