Results 41 to 50 of about 1,720,439 (181)

Nucleotide Modification Alters MicroRNA-Dependent Silencing of MicroRNA Switches

Molecular Therapy: Nucleic Acids, 2019
mRNA therapeutics hold great promise for the treatment of human diseases. While incorporating naturally occurring modified nucleotides during synthesis has greatly increased their potency and safety, challenges in selective expression have hindered ...
John Lockhart, John Canfield, Ezinne Francess Mong, Jeffrey VanWye, Hana Totary-Jain   +4 more
doaj   +1 more source

Eine Universelle Nukleosid‐zu‐Nukleosid‐5′‐triphosphat‐Enzymkaskade angetrieben durch Polyphosphat

Angewandte Chemie, EarlyView.
Nukleosid‐5′‐Triphosphate (NTPs) sind wichtige Bausteine für Nukleinsäuren. Ihre chemische Synthese ist aufgrund der geringen Ausbeute der 5′‐Triphosphorylierungsreaktion nur wenig effizient. Wir stellen hier eine universelle, durch Polyphosphat angetriebene Zwei‐Enzym‐Kaskade vor, die eine direkte Phosphorylierung von Nukleosiden zu NTPs ermöglicht ...
Jonathan P. Suess, Nicolas V. Cornelissen   +1 more
wiley   +1 more source

Guide snoRNAs: Drivers or Passengers in Human Disease?

Biology, 2018
In every domain of life, RNA-protein interactions play a significant role in co- and post-transcriptional modifications and mRNA translation. RNA performs diverse roles inside the cell, and therefore any aberrancy in their function can cause various ...
Manisha Deogharia, Mrinmoyee Majumder
doaj   +1 more source

Pseudouridine Metabolism. IV. Excretion of Pseudouridine and Other Nitrogenous Metabolites in Chronic Leukemia [PDF]

Blood, 1963
Abstract Urinary pseudouridine, uric acid, and creatine have been estimated in a group of patients with chronic lymphocytic leukemia, chronic myelogenous leukemia, and other diseases. The results indicate that there is a selective and specific overproduction of pseudouridine in patients with chronic lymphocytic leukemia.
S M, WEISSMAN, M, LEWIS, M, KARON
openaire   +2 more sources

A Universal Nucleoside‐to‐Nucleoside‐5′‐Triphosphate Enzyme Cascade Driven by Polyphosphate

Angewandte Chemie International Edition, EarlyView.
Nucleoside‐5′‐triphosphates (NTPs) are essential components for the generation of nucleic acids, yet their chemical synthesis is hindered by the low yield of nucleoside phosphorylation. Here, we present a universal, two‐enzyme nucleoside‐to‐NTP cascade that is driven by polyphosphate.
Jonathan P. Suess, Nicolas V. Cornelissen   +1 more
wiley   +1 more source

Definer: A computational method for accurate identification of RNA pseudouridine sites based on deep learning.

PLoS ONE
Pseudouridine is an important modification site, which is widely present in a variety of non-coding RNAs and is involved in a variety of important biological processes.
Bo Han, Sudan Bai, Yang Liu, Jiezhang Wu, Xin Feng, Ruihao Xin   +5 more
doaj   +1 more source

The Role of Cysteine Residues in the Rearrangement of Uridine to Pseudouridine Catalyzed by Pseudouridine Synthase I [PDF]

Journal of Biological Chemistry, 1997
Escherichia coli tRNA pseudouridine synthase I (PSUI) catalyzes the conversion of uridine residues to pseudouridine in positions 38, 39, and 40 of various tRNA molecules. In previous biochemical studies with this enzyme (Kammen, H. O., Marvel, C. C., Hardy, L., and Penhoet, E. E. (1988) J. Biol. Chem.
X, Zhao, D A, Horne
openaire   +2 more sources

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

A Feature Fusion Predictor for RNA Pseudouridine Sites with Particle Swarm Optimizer Based Feature Selection and Ensemble Learning Approach

Current Issues in Molecular Biology, 2021
RNA pseudouridine modification is particularly important in a variety of cellular biological and physiological processes. It plays a significant role in understanding RNA functions, RNA structure stabilization, translation processes, etc.
Xiao Wang, Xi Lin, Rong Wang, Nijia Han, Kaiqi Fan, Lijun Han, Zhaoyuan Ding   +6 more
doaj   +1 more source

Rapid Access to Photoswitchable RNA Binders: Fluorination Enhances Protein Rescue by Exon Inclusion

Chemistry – A European Journal, EarlyView.
An adaptable approach, many light‐switchable RNA binders. We report a versatile strategy for rapidly synthesizing photoswitchable RNA binders. The ligands can target SMN2 pre‐mRNA, restoring the levels of the corresponding protein by exon‐inclusion rescue.
Lei Zhang, Nemanja Djokovic, Alexander Herr, Anna Wacker, Emily Swanson Hay, Daniel Santana, Amanda E. Hargrove, Harald Schwalbe, Katarina Nikolic, Frank Abendroth, Olalla Vázquez   +10 more
wiley   +1 more source