Results 61 to 70 of about 1,720,439 (181)
Biotechnology and Bioengineering, Volume 123, Issue 7, Page 1836-1870, July 2026.ABSTRACT In vitro transcription (IVT) plays a critical role in the manufacture of mRNA vaccines and therapeutics. Optimizing mRNA yield and ensuring product quality, such as capping efficiency and integrity, are essential but mechanistically complex. This study presents a modular mechanistic model of the IVT process to advance scientific understanding ...
Keqi Wang +12 more
wiley +1 more source
High‐throughput Profiling of Pseudouridines in Microbiome‐derived Bacterial RNA
Current Protocols, Volume 6, Issue 7, July 2026.Abstract Pseudouridine (Ψ) is a widespread RNA modification that influences RNA stability, structure, and translation. However, its role in bacterial mRNA, particularly within complex microbiomes, remains poorly defined. Here, we describe a bisulfite‐based sequencing workflow coupled with a scalable computational pipeline for base‐resolution ...
Shikha Sharma +2 more
wiley +1 more source
Molecular Therapy: Nucleic AcidsNucleoside-modified messenger RNA (mRNA) technologies necessarily incorporate N1-methylpseudouridine into the mRNA molecules to prevent the over-stimulation of cytoplasmic RNA sensors.
Esteban Finol +4 more
doaj +1 more source
Journal of Biomedical Materials Research Part A, Volume 114, Issue 7, July 2026.ABSTRACT Messenger RNA (mRNA) therapy represents a transformative platform in regenerative medicine, but its application to skeletal disorders remains limited by the pronounced hepatic tropism of conventional lipid nanoparticle (LNP) delivery systems.
Pengcheng Ren +10 more
wiley +1 more source
Delivery Systems for Therapeutic Genome Editing: Challenges, Innovations, and Future Perspectives
MedComm, Volume 7, Issue 7, July 2026.Schematic illustration of four emerging CRISPR–Cas delivery platforms defined by distinct design principles and structural features: virus‐mimicking nanosystems (e.g., VLPs), cell‐derived extracellular vesicles, cell‐penetrating peptides, and stimuli‐responsive scaffolds. These platforms enable spatiotemporally controlled delivery of RNPs, mRNA, or DNA
Meijia Yang +9 more
wiley +1 more source
RNA Modifications in Tumor Microenvironment: A New Dimension for Cancer Treatment
MedComm, Volume 7, Issue 7, July 2026.Tumor initiation and progression depend on a supportive and highly heterogeneous tumor microenvironment (TME). RNA modifications regulate signaling pathways and cytokine networks that shape TME dynamics, facilitating interactions between cancer cells and noncancerous stromal cells, and ultimately promoting immune evasion and tumor progression ...
Qiwen Li +3 more
wiley +1 more source
Agricultural and Food Science, 1998 The potential of the spot urine sampling technique to assess urinary pseudouridine excretion was evaluated. Twelve multiparous Holstein-Friesian cows were fed two experimental diets in a complete change-over design with two 14 day experimental periods ...
Kevin J. Shingfield, Nicholas W. Offer
doaj
rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences
Yeast, Volume 43, Issue 4, Page 170-183, July 2026.This work introduces a novel approach for analysing long repetitive genomic sequences, combining a method to isolate large DNA molecules from individual chromosomes. We also describe the bioinformatics tool rDNAmine, which enables the isolation of long‐repeat sequences from reads obtained using the Oxford Nanopore method, as well as their preliminary ...
Agnieszka Czarnocka‐Cieciura +1 more
wiley +1 more source
Agricultural and Food Science, 2008 The potential of the spot urine sampling technique to assess urinary pseudouridine excretion was evaluated. Twelve multiparous Holstein-Friesian cows were fed two experimental diets in a complete change-over design with two 14 day experimental periods ...
K. SHINGFIELD, N.W. OFFER
doaj
The Emerging Field of Epitranscriptomics in Neurodevelopmental and Neuronal Disorders
Frontiers in Bioengineering and Biotechnology, 2018 Analogous to DNA methylation and histone modifications, RNA modifications represent a novel layer of regulation of gene expression. The dynamic nature and increasing number of RNA modifications offer new possibilities to rapidly alter gene expression ...
Margarita T. Angelova +6 more
doaj +1 more source