Frontiers in Molecular Biosciences, 2022 Pyrrolysyl-tRNA synthetase (PylRS) is frequently used for site-specific incorporation of noncanonical amino acids (ncAAs) into proteins. Recently, the active site of Methanomethylophilus alvus PylRS (MaPylRS) has been rationally engineered to expand its ...
Jonathan T. Fischer +3 more
doaj +1 more source
Nonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strain. [PDF]
PLoS ONE, 2013 Systematic studies of nonsense and sense suppression of the original and three derivative Methanosarcina mazei PylRS-tRNA(Pyl) pairs and cross recognition between nonsense codons and various tRNA(Pyl) anticodons in the Escherichia coli BL21(DE3) cell ...
Keturah A Odoi +3 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 Introducing non-canonical amino acids (ncAAs) by engineered orthogonal pairs of aminoacyl-tRNA synthetases and tRNAs has proven to be a highly useful tool for the expansion of the genetic code.
Nikolaj G. Koch +4 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNAPyl pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons.
Han-Kai Jiang +11 more
doaj +1 more source
Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids
Frontiers in Genetics, 2023 Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of physiological roles across the tree of life, including in human systems.
Han-Kai Jiang +15 more
doaj +1 more source
An Unnatural Amino Acid-Regulated Growth Controller Based on Informational Disturbance
Life, 2021 We designed a novel growth controller regulated by feeding of an unnatural amino acid, Nε-benzyloxycarbonyl-l-lysine (ZK), using a specific incorporation system at a sense codon. This system is constructed by a pair of modified pyrrolisyl-tRNA synthetase
Yusuke Kato
doaj +1 more source
Cell Reports: Methods, 2023 Summary: Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification.
Birthe Meineke +7 more
doaj +1 more source
Transmission of ‘ Candidatus Phytoplasma pyri’ by naturally infected Cacopsylla pyri to peach, an approach to the epidemiology of peach yellow leaf roll (
Plant Pathology, 2018 Peach orchards in the northeast of Spain were severely affected in 2012 by a previously unreported disease in this area. The symptoms included early reddening, leaf curling, decline, abnormal fruits, and in some cases death of the peach trees. All the infected peach samples were positive for ‘ Candidatus
Sabaté, J. +4 more
openaire +1 more source
Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming. [PDF]
Chem RevDunkelmann DL, Chin JW.
europepmc +2 more sources
Convenient Genetic Encoding of Phenylalanine Derivatives through Their α-Keto Acid Precursors
Biomolecules, 2021 The activity and function of proteins can be improved by incorporation of non-canonical amino acids (ncAAs). To avoid the tedious synthesis of a large number of chiral phenylalanine derivatives, we synthesized the corresponding phenylpyruvic acid ...
Li Liu +8 more
doaj +1 more source