Results 21 to 30 of about 810 (104)
Structural basis for the site-specific incorporation of lysine derivatives into proteins. [PDF]
PLoS ONE, 2014 Posttranslational modifications (PTMs) of proteins determine their structure-function relationships, interaction partners, as well as their fate in the cell and are crucial for many cellular key processes.
Veronika Flügel +2 more
doaj +1 more source
Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction. [PDF]
PLoS ONE, 2014 Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe.
Jessica Torres-Kolbus +3 more
doaj +1 more source
Frontiers in Chemistry, 2021 This study reports the application of expanding genetic codes in developing protein cage-based delivery systems. The evolved Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)•tRNAPyl pairs derived from directed evolution are examined to probe their
Yi-Hui Wang +9 more
doaj +1 more source
Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines
Insects, 2021 Inducible gene expression systems are important tools for studying gene function and to control protein synthesis. With the completion of the detailed map of the silkworm (Bombyx mori) genome, the study of Bombyx mori has entered the post-genome era ...
Wei Lu +4 more
doaj +1 more source
Evolution of Pyrrolysyl-tRNA Synthetase: From Methanogenesis to Genetic Code Expansion. [PDF]
Chem RevKoch NG, Budisa N.
europepmc +2 more sources
First Report of ‘Candidatus Phytoplasma pyri’ Causing Peach Yellow Leaf Roll (PYLR) in Spain
Plant Disease, 2014 ‘Candidatus Phytoplasma prunorum,’ which causes European stone fruit yellows (ESFY), is the prevalent phytoplasma affecting Prunus spp. in Europe. It is closely related to ‘Ca. P. pyri,’ which causes pear decline (PD) in pear trees. Both phytoplasma belong to the ribosomal group 16Sr-X and are naturally transmitted by different species of Cacopsylla ...
J, Sabaté, A, Laviña, A, Batlle
openaire +2 more sources
Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins
Molecules, 2016 The increasing need for site-specific protein decorations that mimic natural posttranslational modifications requires access to a variety of noncanonical amino acids with moieties enabling bioorthogonal conjugation chemistry.
Matthias P. Exner +9 more
doaj +1 more source
Bioresources and Bioprocessing, 2023 Aminoacyl-tRNA synthetase (aaRS) is a core component for genetic code expansion (GCE), a powerful technique that enables the incorporation of noncanonical amino acids (ncAAs) into a protein. The aaRS with polyspecificity can be exploited in incorporating
Ke Liu +9 more
doaj +1 more source
Molecules, 2018 Genetic code expansion has largely relied on two types of the tRNA—aminoacyl-tRNA synthetase pairs. One involves pyrrolysyl-tRNA synthetase (PylRS), which is used to incorporate various lysine derivatives into proteins.
Atsushi Yamaguchi +3 more
doaj +1 more source
Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion
Croatica Chemica Acta, 2016 Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl‐tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids ...
Ana Crnković +3 more
doaj +1 more source