Results 31 to 40 of about 810 (104)

An Expanded Toolbox for Versatile Chemical Editing of Adeno‐Associated Virus

Angewandte Chemie, Volume 138, Issue 7, 9 February 2026.
We describe technology to introduce diverse non‐natural chemical functionalities site‐specifically into the capsid of adeno‐associated virus through genetic code expansion, and using them to engineer this leading vector for human gene therapy for enhanced tissue specificity and reduced immunogenicity Abstract Site‐specific incorporation of noncanonical
Quan Pham, Jake Glicksman, Boyang Han, David Koo, Conor Loynd, Soumya Jyoti Singha Roy, Abhishek Chatterjee   +6 more
wiley   +2 more sources

Towards Reassignment of the Methionine Codon AUG to Two Different Noncanonical Amino Acids in Bacterial Translation

Croatica Chemica Acta, 2016
Genetic encoding of noncanonical amino acids (ncAAs) through sense codon reassignment is an efficient tool for expanding the chemical functionality of proteins. Incorporation of multiple ncAAs, however, is particularly challenging.
Alessandro De Simone, Carlos G. Acevedo-Rocha, Michael Georg Hoesl, Nediljko Budisa   +3 more
doaj   +1 more source

Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids

Angewandte Chemie International Edition, EarlyView.
Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar, Manas Joshi, Abhishek Chatterjee   +2 more
wiley   +1 more source

A Genetically Encoded Homocysteine Precursor to Probe Protein Active Sites and to Addict Escherichia coli to a Noncanonical Amino Acid Directly Involved in Catalysis

Angewandte Chemie, Volume 137, Issue 36, September 1, 2025.
A new genetically encoded amino acid is chemically converted into homocysteine (Hcy) under mild conditions to probe and to activate protein active sites. Surprisingly, Hcy can replace cysteine or serine residues in several enzymes. Its potential for a widespread use in proteins is further underlined by rendering Escherichia coli dependent on Hcy ...
Clara Dunker, Henning D. Mootz
wiley   +2 more sources

Aufklärung des Wirkmechanismus der Synthetase: Das Dimer der Pyrrolysyl‐tRNA‐Synthetase nutzt sekundäre Bindungsstellen in der Zelle

Angewandte Chemie, Volume 138, Issue 18, 27 April 2026.
Das Dimer der Pyrrolysyl‐tRNA‐Synthetase zeigt im katalytischen Zyklus einen alternierenden Wirkmodus seiner Monomere, der durch die Belegung sekundärer Bindungsstellen an den Intermonomer‐Grenzflächen außerhalb der katalytischen Bindungsstelle realisiert wird.
Jessica Dröden, Christoph Globisch, Eliane Landwehr, Theresa S. Braun, Daniel Summerer, Christine Peter, Malte Drescher   +6 more
wiley   +1 more source

Optimized Directed Evolution of E. coli leucyl‐tRNA Synthetase adds many Noncanonical Amino Acids into the Eukaryotic Genetic Code Including Ornithine and Nϵ‐Acetyl‐Methyllysine

Angewandte Chemie, Volume 137, Issue 14, April 1, 2025.
The pyrrolysyl pair has dominated noncanonical amino acids (ncAAs) incorporation in eukaryotes, but additional engineerable pairs are needed to further expand this toolbox. Although E. coli leucyl‐tRNA synthetase (EcLeuRS) is a promising candidate, engineering its substrate specificity has been much less successful.
Elise D. Ficaretta, Tarah J. Yared, Subrata Bhattacharjee, Lena A. Voss, Rachel L. Huang, Abhishek Chatterjee   +5 more
wiley   +2 more sources

Genetische Codierung von Pentafluorphosphato‐Phenylalanin ermöglicht die Herstellung von PF5‐Proteinen als Phosphoprotein‐Mimetika

Angewandte Chemie, Volume 138, Issue 1, 2 January 2026.
Erweiterung des genetischen Codes mit einem einzigartigen hyperfluorierten Phosphotyrosin‐Analogon. In dieser Arbeit haben wir die nicht‐natürliche Aminosäure Pentafluorphosphato‐Difluormethyl‐Phenylalanin, die sieben Fluoratome und eine permanente negative Ladung trägt, mittels mutierter, orthogonaler Aminoacyl‐tRNA‐Synthetasen erfolgreich in drei ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source

Genetic Encoding of Pentafluorophosphato‐Phenylalanine Provides PF5‐Proteins as Phosphoprotein Mimetics

Angewandte Chemie International Edition, Volume 65, Issue 1, 2 January 2026.
Genetic code expansion with a unique hyper‐fluorinated phosphotyrosine analog. In this work, we successfully incorporated the unnatural amino acid pentafluorophosphato‐difluoromethyl‐phenylalanine, carrying seven fluorine atoms and a permanent negative charge into three different proteins via the use of mutated orthogonal aminoacyl‐tRNA synthetases ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies, Tim Moritz Weber, Ivana Drienovská   +2 more
wiley   +1 more source

Correlating membrane‐protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single‐molecule FRET

Protein Science, Volume 34, Issue 11, November 2025.
Abstract We present a strategy that deploys structural bioinformatics, molecular simulation, and single‐molecule Förster Resonance Energy Transfer (FRET) microscopy for observing the ligand‐dependent conformational dynamics of integral membrane proteins in situ.
Hugh R. Higinbotham, Christine A. Arbour, Barbara Imperiali   +2 more
wiley   +1 more source