Results 21 to 30 of about 124,832 (334)

The contribution of two isozymes to the pyruvate kinase activity of Vibrio cholerae: One K+-dependent constitutively active and another K+-independent with essential allosteric activation. [PDF]

PLoS ONE, 2017
In a previous phylogenetic study of the family of pyruvate kinase EC (2.7.1.40), a cluster with Glu117 and another with Lys117 were found (numbered according to the rabbit muscle enzyme).
Carlos Guerrero-Mendiola, José J García-Trejo, Rusely Encalada, Emma Saavedra, Leticia Ramírez-Silva   +4 more
doaj   +1 more source

The Allosteric Regulation of Pyruvate Kinase [PDF]

Journal of Biological Chemistry, 2000
Pyruvate kinase (PK) is critical for the regulation of the glycolytic pathway. The regulatory properties of Escherichia coli were investigated by mutating six charged residues involved in interdomain salt bridges (Arg(271), Arg(292), Asp(297), and Lys(413)) and in the binding of the allosteric activator (Lys(382) and Arg(431)).
VALENTINI, GIOVANNA, CHIARELLI, LAURENT, SPERANZA, MARIA LUISA, GALIZZI, ALESSANDRO, MATTEVI, ANDREA, FORTIN R   +5 more
openaire   +5 more sources

The allosteric regulation of pyruvate kinase [PDF]

FEBS Letters, 1996
Crystallographic and mutagenesis studies have unravelled the general features of the allosteric transition mechanism in pyruvate kinase. The enzyme displays a dramatic conformational change in going from the T‐ to the R‐state. All three domains forming each subunit of the tetrameric enzyme undergo simultaneous and concerted rotations, in such a way ...
MATTEVI, ANDREA, VALENTINI, GIOVANNA, BOLOGNESI M.   +2 more
openaire   +4 more sources

Pyruvate Kinase Revisited [PDF]

Journal of Biological Chemistry, 2005
Desde hace más de 50 años, se sabe que el K+ es un activador esencial de la piruvato quinasa (Kachmar, J. F., y Boyer, P. D. (1953) J. Biol. Chem. 200, 669-683). Sin embargo, el papel de K+ en la catálisis por piruvato quinasa no se ha entendido completamente.
Jesús Oria‐Hernández, Nallely Cabrera, Ruy Pérez‐Montfort, Leticia Ramírez‐Silva   +3 more
openaire   +3 more sources

A novel homozygous missense variant p.D339N in the PKLR gene correlates with pyruvate kinase deficiency in a Pakistani family: a case report

Journal of Medical Case Reports, 2022
Background Pyruvate kinase deficiency is an exceptionally rare autosomal recessive Mendelian disorder caused by bi-allelic pathogenic variants in the PKLR gene.
Atta Ur Rehman, Abdur Rashid, Zubair Hussain, Khadim Shah   +3 more
doaj   +1 more source

Characterizing the Effect of the Lysine Deacetylation Modification on Enzyme Activity of Pyruvate Kinase I and Pathogenicity of Vibrio alginolyticus

Frontiers in Veterinary Science, 2022
Pyruvate kinase I (PykF) is one of the key enzymes of glycolysis and plays a crucial role in bacterial metabolism. Several acetylation sites of Vibrio alginolyticus PykF were reported in previous studies and then 11 sites were first verified in this ...
Zhou Xu, Zhou Xu, Zhou Xu, Linjing Wang, Linjing Wang, Linjing Wang, Xudong Wang, Xudong Wang, Xudong Wang, Mingyue Wan, Mingyue Wan, Mingyue Wan, Mei Tang, Mei Tang, Mei Tang, Yu Ding, Yu Ding, Yu Ding   +17 more
doaj   +1 more source

Research Progress on the Effect of Post-translational Modification of Pyruvate Kinase on Meat Quality [PDF]

Shipin Kexue
The postmortem conversion of muscle to meat goes through a series of complex physiological and biochemical changes, among which protein post-translational modifications (PTMs) are key factors affecting meat quality.
HUANG Xiaolan, CHEN Li, REN Chi, BAI Yuqiang, HOU Chengli, LI Xin, LUO Ruiming, ZHANG Dequan
doaj   +1 more source

Incorporating expression data in metabolic modeling: a case study of lactate dehydrogenase [PDF]

J Theor Biol 240: 464-474 (2006), 2005
Integrating biological information from different sources to understand cellular processes is an important problem in systems biology. We use data from mRNA expression arrays and chemical kinetics to formulate a metabolic model relevant to K562 erythroleukemia cells. MAP kinase pathway activation alters the expression of metabolic enzymes in K562 cells.
arxiv   +1 more source

Specific tumor-derived CCL2 mediated by pyruvate kinase M2 in colorectal cancer cells contributes to macrophage recruitment in tumor microenvironment

Tumor Biology, 2017
Development of colorectal cancer has been considered as a result of imbalance of pro- and anti-inflammatory intestinal microenvironment accompanied by macrophage recruitment.
Kejian Zou, Yaodong Wang, Yan Hu, Liansheng Zheng, Wanfu Xu, Guoxin Li   +5 more
doaj   +1 more source

Pyruvate Kinase, Inflammation and Periodontal Disease

Pathogens, 2021
Pyruvate kinase (PK) is the final and rate-limiting enzyme in glycolysis. It has four isoforms PKM1, PKM2, PKL and PKR. PK can form homo tetramers, dimers or monomers. The tetrameric form has the most catalytic activity; however, the dimeric form has non-
Melissa M. Grant
doaj   +1 more source