Results 1 to 10 of about 1,004,764 (341)

Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]

, 2020
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer, Garber, Megan E, Hillson, Nathan J, Mukhopadhyay, Aindrila   +3 more
core   +1 more source

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping [PDF]

BMC Genomics, 2021
AbstractBackgroundThe detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification ...
Baowei Li, Yanran Liu, Xiaodan Hao, Jinhua Dong, Limei Chen, Haimei Li, Wei Wu, Ying Liu, Jianxun Wang, Yin Wang, Peifeng Li   +10 more
openaire   +3 more sources

Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives [PDF]

, 2015
This is a final draft version of the publication following acceptance by the journal.Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many ...
Karteris, E, Rogers-Broadway, KR
core   +1 more source

Multi-parallel qPCR provides increased sensitivity and diagnostic breadth for gastrointestinal parasites of humans: field-based inferences on the impact of mass deworming [PDF]

, 2016
BACKGROUND: Although chronic morbidity in humans from soil transmitted helminth (STH) infections can be reduced by anthelmintic treatment, inconsistent diagnostic tools make it difficult to reliably measure the impact of deworming programs and often miss
A Ebrahim, A Jeandron, AC Clements, AJ Branscum, Alice V. Easton, B Levecke, B Nikolay, BA Boatin, Cassian Mwatele, Charles S. Mwandawiro, CR Stensvold, CV Holland, D Croce, D Engels, D Glinz, D Mupfasoni, DA Bundy, DA Bundy, DB Elkins, DJ Lunn, DM Deer, Elise M. O’Connell, FA Jonker, FL Santos, HL Guyatt, J Martin, JE Forrester, Jimmy H. Kihara, JJ Verweij, JJ Verweij, Joanne P. Webster, JP Webster, JS McCarthy, KE Hoagland, LM Assefa, LS Diamond, M Albonico, M Basuni, M Booth, M Taniuchi, Maurice R. Odiere, MO Harhay, N Katz, N Katz, NA Croll, P Hotez, PH Lamberton, R Anderson, R Bergquist, R Mejia, RD Pearson, Rita G. Oliveira, RJ Hove ten, RK Prichard, RL Pullan, RL Pullan, RM Anderson, RM Anderson, RO Cimino, Roy M. Anderson, S Brooker, S Brooker, S Knopp, S Knopp, S Knopp, S Lustigman, S Williams-Blangero, Sammy M. Njenga, SC Knowles, Simon J. Brooker, SJ Krauth, Stella Kepha, Thomas B. Nutman, TW Jia, WHO, XP Ye   +75 more
core   +3 more sources

Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]

, 2019
BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak, A Bak, Aurélie Bak, AYT Wong, BM Maghari, C Wolf, E Kamau, G Cottenet, G Cottenet, HD VanGuilder, IB Salisu, IMJ Scholtens, J Davison, JB Emerson, Joanne B. Emerson, K Arumuganathan, K Nybo, K Ramessar, L Garibyan, M Chaouachi, M Singh, M Tajadini, MA Fraiture, MJ Holden, MJ Oliver, Q Zhang, R Becker, RJ Pryor, S Broeders, S Fernandez, S Llewellyn, SA Bustin, T Demeke, W Fu, Y Hou, Y Wu   +35 more
core   +1 more source

Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR. [PDF]

, 2011
Telomere length/DNA content has been measured in epidemiological/clinical settings with the goal of testing a host of hypotheses related to the biology of human aging, but often the conclusions of these studies have been inconsistent.
Aviv, Abraham, Blackburn, Elizabeth, Cao, Xiaojian, Hunt, Steven C, Kimura, Masayuki, Lin, Jue   +5 more
core   +2 more sources

Copy number variations in healthy subjects. Case study: iPSC line CSSi005-A (3544) production from an individual with variation in 15q13.3 chromosome duplicating gene CHRNA7 [PDF]

, 2018
CHRNA7, encoding the neuronal alpha7 nicotinic acetylcholine receptor (a7nAChR), is highly expressed in the brain, particularly in the hippocampus. It is situated in the 15q13.3 chromosome region, frequently associated with a Copy Number Variation (CNV),
Altieri, Filomena, Bernardini, Laura, Della Monica, Matteo, Ferrari, Daniela, Goldoni, Marina, Lamorte, Giuseppe, Mazzoccoli, Gianluigi, Postorivo, Diana, Rosati, Jessica, Tata, Ada Maria, Torres, Barbara, Turco, Elisa Maria, Vescovi, Angelo Luigi, Vinci, Ersilia   +13 more
core   +1 more source

BPA-Induced Deregulation of Epigenetic Patterns: Effects on Female Zebrafish Reproduction [PDF]

, 2016
Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms.
Carnevali, Oliana, Cobellis, Gilda, DALLA VALLE, Luisa, Gioacchini, Giorgia, Maradonna, Francesca, Piccinetti, Chiara Carla, Santangeli, Stefania   +6 more
core   +1 more source

Development and field assessment of a quantitative PCR for the detection and enumeration of the noxious bloom-former Anabaena planktonica [PDF]

, 2007
Anabaena planktonica is a harmful, bloom-forming freshwater cyanobacterium, which has arrived recently in New Zealand. In the short time since its incursion (
Cary, S. Craig, Rueckert, Andreas, Wood, Susanna A.   +2 more
core   +1 more source

Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments [PDF]

, 2019
Soil nematode communities and food web indices can inform about the complexity, nutrient flows and decomposition pathways of soil food webs, reflecting soil quality.
De Caceres M., De Goede R. G. M., Edgar R. C., Ferris H., Ferris H., Köppen W., Mekonen S., Minton N. A., Moura G. S., Neher D. A., Neher D. A., Neher D. A., Niles R. K., Oksanen J., Oostenbrink M., Pan F., Pinheiro J., R Development Core Team, RStudio Team, Toth G., Wang K.‐H., WRB, I. W. G., Yeates G. W.   +22 more
core   +1 more source