Results 31 to 40 of about 596,924 (249)

Selective abiotic and biotic stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plants in Lampung, Indonesia

open access: yesJournal of Tropical Soils, 2023
Indonesia has faced extensive poor soil quality and health for years.Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil's essential life-supporting system, such as the functional gene pool ...
Dori Kusuma Jaya   +3 more
doaj   +1 more source

SPUD qPCR Assay Confirms PREXCEL-Q Software's Ability to Avoid qPCR Inhibition [PDF]

open access: yesCurrent Issues in Molecular Biology, 2010
Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends.
Gallup, Jack   +3 more
openaire   +3 more sources

Specific Primer Design for Detection and Quantification of Entomopathogenic Fungi Metarhizium anisopliae using Quantitative PCR (qPCR) in Soil and Cocoon Samples

open access: yesAGRIVITA Journal of Agricultural Science
The information on the abundance and dynamics of entomopathogenic fungus Metarhizium anisopliae on the soil is limited by the existence of insect hosts. In this study, to detect and quantify specifically for M.
Syaiful Amri Saragih   +2 more
doaj   +1 more source

Development of an Interferon Gamma Response-Related Signature for Prediction of Survival in Clear Cell Renal Cell Carcinoma

open access: yesJournal of Inflammation Research, 2021
Lixiao Liu,1,* Xuedan Du,2,* Jintao Fang,3,* Jinduo Zhao,1 Yong Guo,3 Ye Zhao,1 Chengyang Zou,1 Xiaojian Yan,1 Wenfeng Li2 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou ...
Liu L   +8 more
doaj  

Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees

open access: yesJournal of Apicultural Science, 2019
Apis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees and its prevalence and relationship with other parasites is poorly known.
Quintana Silvina   +9 more
doaj   +1 more source

Comparative assessment of the copy number of satellite repeats in the genome of Triticeae species

open access: yesВавиловский журнал генетики и селекции, 2023
Satellite repeats are a significant component of the genome of Triticeae and play a crucial role in the speciation. They are a valuable tool for studying these processes.
P. Yu. Kroupin   +5 more
doaj   +1 more source

A beginner’s guide to RT-PCR, qPCR and RT-qPCR [PDF]

open access: yesThe Biochemist, 2020
The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative ...
openaire   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

open access: yesFEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang   +8 more
wiley   +1 more source

Molecular genetic detection and differentiation of Xanthomonas oryzae pv. oryzicola, bacterial leaf streak agents of rice

open access: yesВавиловский журнал генетики и селекции, 2022
The genus Xanthomonas comprises phytopathogenic bacteria which infect about 400 host species, including a wide variety of economically important plants. Xanthomonas oryzae pv.
M. L. Koroleva   +4 more
doaj   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

open access: yesFEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

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