Results 41 to 50 of about 1,004,764 (341)
Biomolecular Detection and Quantification, 2017 Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative ...
Bustin, Stephen A., Huggett, Jim
openaire +2 more sources
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices [PDF]
, 2015 Background: In order to provide a system fully integrated with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMOs in food/feed matrices, two bidirectional DNA walking ...
De Loose, Marc +6 more
core +6 more sources
Insights into pneumococcal pneumonia using lung aspirates and nasopharyngeal swabs collected from pneumonia patients in The Gambia. [PDF]
, 2020 We investigated the pathogenesis of pneumococcal pneumonia using clinical specimens collected for pneumonia surveillance in The Gambia. Lung aspirates and nasopharyngeal swabs from 31 patients were examined by culture, qPCR, whole genome sequencing ...
Dunne, EM +10 more
core +4 more sources
Environmental DNA, 2020 Considerable promise and excitement exist in the application of environmental DNA (eDNA) methods to environmental monitoring and species inventories as eDNA can provide cost-effective and accurate biodiversity information. However, considerable variation
V. S. Langlois +4 more
semanticscholar +1 more source
Sequence-specific protein aggregation generates defined protein knockdowns in plants [PDF]
, 2016 Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form beta-structured inclusions.
Aesaert, Stijn +16 more
core +2 more sources
BMC Biotechnology, 2020 This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR).
P. Foo +6 more
semanticscholar +1 more source
SPUD qPCR Assay Confirms PREXCEL-Q Software's Ability to Avoid qPCR Inhibition [PDF]
Current Issues in Molecular Biology, 2010 Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends.
Gallup, Jack +3 more
openaire +3 more sources
High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells. [PDF]
, 2017 Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes.
Arias, Angelo +12 more
core +2 more sources
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs [PDF]
, 2016 Medulloblastoma (MB) is the most common malignant brain tumor of pediatric age and is characterized by cells expressing stem, astroglial, and neuronal markers.
Alfano, Vincenzo +13 more
core +4 more sources