Results 31 to 40 of about 53,016 (292)

Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens

Journal of Clinical Microbiology, 2020
On 31 December 2019, the World Health Organization was informed of a cluster of cases of pneumonia of unknown etiology in Wuhan, China. Subsequent investigations identified a novel coronavirus, now named severe acute respiratory syndrome coronavirus 2 ...
J. Chan, C. Yip, K. To, T. H. Tang, S. C. Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, A. C. Ng, Zijiao Zou, H. Tsoi, G. K. Choi, A. Tam, V. Cheng, Kwok-Hung Chan, O. Tsang, K. Yuen   +15 more
semanticscholar   +1 more source

Potential SARS-CoV-2 RdRp inhibitors of cytidine derivatives: Molecular docking, molecular dynamic simulations, ADMET, and POM analyses for the identification of pharmacophore sites

PLoS ONE, 2022
The RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 is one of the optimum targets for antiviral drug design and development. The hydroxyl groups of cytidine structures were modified with different aliphatic and aromatic groups to obtain 5´-O-acyl and 2´
Sarkar M A Kawsar, M. Hosen, Sajjad Ahmad, Youness El Bakri, Hamid Laaroussi, T. Ben Hadda, F. Almalki, Y. Ozeki, S. Goumri‐Said   +8 more
semanticscholar   +1 more source

SARS-CoV-2 RNA dependent RNA polymerase (RdRp) targeting: an in silico perspective

Journal of Biomolecular Structure and Dynamics, 2020
New treatment against SARS-CoV-2 now is a must. Nowadays, the world encounters a huge health crisis by the COVID-19 viral infection. Nucleotide inhibitors gave a lot of promising results in terms of its efficacy against different viral infections.
Abdo A. Elfiky
semanticscholar   +1 more source

Rapidly self-deoxygenating controlled radical polymerization in water via in situ disproportionation of Cu(i) [PDF]

, 2020
Rapidly self-deoxygenating Cu-RDRP in aqueous media is investigated. The disproportionation of Cu(I)/Me6Tren in water towards Cu(II) and highly reactive Cu(0) leads to O2-free reaction environments within the first seconds of the reaction, even when the ...
Haddleton, David M., Han, Yisong, Liarou, Evelina, Sánchez, Ana M., Walker, Marc   +4 more
core   +1 more source

Coronavirus replication–transcription complex: Vital and selective NMPylation of a conserved site in nsp9 by the NiRAN-RdRp subunit

Proceedings of the National Academy of Sciences of the United States of America, 2021
Significance We report an intersubunit interaction within the coronavirus replication–transcription complex that is critical for replication and evolutionarily conserved.
H. Slanina, Ramakanth Madhugiri, Ganesh Bylapudi, Karin Schultheiß, Nadja Karl, Anastasia A. Gulyaeva, A. Gorbalenya, U. Linne, J. Ziebuhr   +8 more
semanticscholar   +1 more source

Aging correlates with lower threshold cycle values for RdRP/RdRP+S genes during molecular detection of SARS-CoV-2

Experimental Gerontology, 2021
RT-qPCR is the most reliable molecular method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we analyzed results of RT-qPCR obtained for 3044 patients diagnosed as SARS-CoV-2-positive using four different molecular tests utilizing five RNA sequences.
Mikuła-Pietrasik, Justyna, Książek, Krzysztof   +1 more
openaire   +2 more sources

Analysis the Effectiveness of Remdesivir, Galidesivir, Sofosbuvir, Tenofovir and Ribavirin as Potential Therapeutic Drug target against SARS-Cov-2 RNA-Dependent RNA Polymerase (RdRp): An in Silico Docking Study [PDF]

Journal of Research in Applied and Basic Medical Sciences, 2023
Background & Aims:  The active site of RdRp-CoV is highly conserved, with two successive and surface-accessible aspartates in a beta-turn structure. Antiviral drugs Remdesivir, Galidesivir, Tenofovir, Sofosbuvir, and Ribavirin are known as inhibitors of ...
Rajneesh Prajapat, Suman Jain
doaj  

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

The EuroBiotech Journal, 2021
A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2.
Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer,   +10 more
doaj   +1 more source

RNA-Dependent RNA Polymerase of the Second Human Pegivirus Exhibits a High-Fidelity Feature

Microbiology Spectrum, 2022
The virus-encoded RNA-dependent RNA polymerase (RdRp) is responsible for viral replication, and its fidelity is closely related to viral diversity, pathogenesis, virulence, and fitness.
Shuyi Chen, Jiqin Wu, Xiaofeng Yang, Qiuli Sun, Su Liu, Farooq Rashid, Emmanuel Enoch Dzakah, Haiying Wang, Jufang Wang, Peng Gong, Shixing Tang   +10 more
doaj   +1 more source

Remdesivir MD Simulations Suggest a More Favourable Binding to SARS-CoV-2 RNA Dependent RNA Polymerase Mutant P323L Than Wild-Type

Biomolecules, 2021
SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) protein is the target for the antiviral drug Remdesivir (RDV). With RDV clinical trials on COVID-19 patients showing a reduced hospitalisation time.
Anwar Mohammad, Fahd Al-Mulla, Dong-Qing Wei, Jehad Abubaker   +3 more
doaj   +1 more source