Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings [PDF]
, 2017 Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories.
Armson, B. +14 more
core +1 more source
Journal of Infection in Developing Countries, 2021 Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira +9 more
doaj +1 more source
Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]
, 2009 Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E. +3 more
core +2 more sources
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis [PDF]
Journal of Visualized Experiments, 2019 Molecular assays are rapid, sensitive and specific, and have become central to diagnosing rabies. PCR based assays have been utilized for decades to confirm rabies diagnosis but have only recently been accepted by the OIE (World Organisation for Animal Health) as a primary method to detect rabies infection.
Denise A, Marston +6 more
openaire +2 more sources
A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses [PDF]
, 2010 In order to establish an accurate, ready-to-use assay for simultaneous detection of Eastern equine encephalitis virus (EEEV) and Western equine encephalitis virus (WEEV), we developed one duplex TaqMan real-time reverse transcriptase polymerase chain ...
Fang Lin +8 more
core +2 more sources
Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus
浙江大学学报. 农业与生命科学版, 2010 A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin +4 more
doaj +1 more source
Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]
Archives of Biological Sciences, 2008 Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B. +4 more
doaj +1 more source
Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection [PDF]
, 2017 Objectives: Feline leukaemia virus (FeLV), a gamma retrovirus, causes diseases of the feline haematopoietic system that are invariably fatal. Rapid and accurate testing at the point-of-need (PON) supports prevention of virus spread and management of ...
Anis, Eman +8 more
core +1 more source
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control [PDF]
, 2011 <p>The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories.
Beer, Martin +18 more
core +7 more sources
SAS programs for real-time RT-PCR having multiple independent samples
BioTechniques, 2004 Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook +4 more
doaj +1 more source