Results 61 to 70 of about 574,812 (244)

Quantitation of rabbit cytokine mRNA by real-time RT-PCR [PDF]

Cytokine, 2007
Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues.
Charmie, Godornes, Brandon Troy, Leader, Barbara J, Molini, Arturo, Centurion-Lara, Sheila A, Lukehart   +4 more
openaire   +2 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Virus culture and real-time RT-PCR in identifying influenza viruses from influenza-like illness cases in Indonesia 2007-2008

Health Science Journal of Indonesia, 2012
Latar belakang: Adanya perbedaan hasil antara kultur virus dengan real-time polymerase chain reaction (RT-PCR) yang digunakan dalam surveilans influenza-like illness (ILI) menunjukkan perlunya mengevaluasi hasil kultur virus yang didapatkan dengan hasil ...
Irene L. Indalao, Vivi Setiawaty, Hana A. Pawestri, Subangkit Subangkit   +3 more
doaj   +3 more sources

Japanese encephalitis virus real-time RT-PCR v2 [PDF]

, 2017
A real-time RT-PCR using an MGB probe, this assay detects Japanese encephalitis virus (JEV) from human and mosquito samples. The assay targets the 3'UTR region of known JEV strains.
Judy A Northill, Mitchell Finger, Michael Lyon, Ian M Mackay   +3 more
openaire   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

LN34 real-time RT-PCR protocol v2

, 2018
Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations [1].
Crystal M. Gigante, Kimberly Wilkins, Rene Edgar Condori Condori, Ashutosh Wadhwa, Yu Li   +4 more
openaire   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

LN34 real-time RT-PCR protocol v1

, 2018
Purpose To describe the LN34 real-time RT-PCR assay procedure used for the qualitative detection of lyssavirus RNA in whole RNA extracted from brain tissue samples. The assay detects RNA from diverse lyssaviruses at varying concentrations [1].
Crystal M. Gigante, Kimberly Wilkins, Rene Edgar Condori Condori, Ashutosh Wadhwa, Yu Li   +4 more
openaire   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Quantification of bovine cytokine gene expression using real-time RT-PCR methodology

Genetics and Molecular Biology, 2007
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis ...
Lilian Giotto Zaros, Patrízia Ana Bricarello, Alessandro Francisco Talamini Amarante, Luiz Lehmann Coutinho   +3 more
doaj   +1 more source