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多成分結晶系に着目した甘味成分Rebaudioside Dの溶解性向上Some of the next articles are maybe not open access.
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Plant Physiology and Biochemistry, 2021
Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S.
Xiaochen Jia, Heng Yin, Heng Yin
exaly +3 more sources
Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S.
Xiaochen Jia, Heng Yin, Heng Yin
exaly +3 more sources
International Journal of Biological Macromolecules, 2020
Rebaudioside D is a promising sweetener due to its zero calorie and high sweetness. Here, a transglucosylase gene eugt11 from Oryza sativa was for the first time expressed in Pichia pastoris, and transformant XE-3 showed the highest expression levels in pH 5.5 BMMY media containing 0.75% methanol.
Jiefang Hong, Siyuan Ma, Tong Huang
exaly +3 more sources
Rebaudioside D is a promising sweetener due to its zero calorie and high sweetness. Here, a transglucosylase gene eugt11 from Oryza sativa was for the first time expressed in Pichia pastoris, and transformant XE-3 showed the highest expression levels in pH 5.5 BMMY media containing 0.75% methanol.
Jiefang Hong, Siyuan Ma, Tong Huang
exaly +3 more sources
Journal of Agricultural and Food Chemistry, 2019
In this study, a monoglucosyl rebaudioside A product was isolated from the mixture of glucosylated rebaudioside A obtained from the most reported and industrial used cyclodextrin glycosyl transferase, Toruzyme 3.0 L (CGTase, Toruzyme 3.0 L). The molecular structure of the monoglucosyl rebaudioside A was characterized using LC-MS/MS and methylation ...
Qingbin Guo +2 more
exaly +3 more sources
In this study, a monoglucosyl rebaudioside A product was isolated from the mixture of glucosylated rebaudioside A obtained from the most reported and industrial used cyclodextrin glycosyl transferase, Toruzyme 3.0 L (CGTase, Toruzyme 3.0 L). The molecular structure of the monoglucosyl rebaudioside A was characterized using LC-MS/MS and methylation ...
Qingbin Guo +2 more
exaly +3 more sources
Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 and in situ UDP-glucose regeneration
Food Chemistry, 2018Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4-0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside.
Liangliang Chen +2 more
exaly +3 more sources
Biochemical Engineering Journal, 2020
Abstract Engineering of enzymes without crystal structures and homologous structures is a great challenge for structure-based computational enzyme design. In this study, a computational strategy that combines protein structure prediction, sequence optimization, and molecular dynamics simulation was developed to improve the catalytic efficiency of the
Min Lin, Fei Wang, Yushan Zhu
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Abstract Engineering of enzymes without crystal structures and homologous structures is a great challenge for structure-based computational enzyme design. In this study, a computational strategy that combines protein structure prediction, sequence optimization, and molecular dynamics simulation was developed to improve the catalytic efficiency of the
Min Lin, Fei Wang, Yushan Zhu
openaire +1 more source
Biotechnology Journal, 2023
Bioconversion of Rebaudioside D faces high-cost obstacles. Herein, a novel glycosyltransferase StUGT converting Rebaudioside A to Rebaudioside D was screened and characterized, which exhibits stronger affinity and substrate specificity for Rebaudioside A than previously reported enzymes.
Siyuan Ma, Yuanyuan Ma
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Bioconversion of Rebaudioside D faces high-cost obstacles. Herein, a novel glycosyltransferase StUGT converting Rebaudioside A to Rebaudioside D was screened and characterized, which exhibits stronger affinity and substrate specificity for Rebaudioside A than previously reported enzymes.
Siyuan Ma, Yuanyuan Ma
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Regulatory Toxicology and Pharmacology, 2014
The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd, Purkayastha +5 more
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The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd, Purkayastha +5 more
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Metabolism and Toxicity Studies Supporting the Safety of Rebaudioside D
International Journal of Toxicology, 2013Rebaudioside D (Reb D) is one of the several glycosides found in the leaves of Stevia rebaudiana (Bertoni) Bertoni (Compositae) which has been identified as a potential sweetener. The metabolism of Reb A and Reb D was evaluated in various in vitro matrices (simulated gastrointestinal fluids, rat liver microsomes, and rat cecal contents) and through ...
Andrey I, Nikiforov +3 more
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Journal of Agricultural and Food Chemistry
Rebaudioside M (Reb M), a zero-calorie sweetener with high sweetness, faces production challenges due to its low yield and purity. UGT76G1, a uridine diphosphate glucose (UDPG)-dependent glycosyltransferase, forms a β-1,3-glycosidic bond with rebaudioside D (Reb D) to produce Reb M but with an efficiency lower than that for stevioside (ST).
Fangwei Song +7 more
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Rebaudioside M (Reb M), a zero-calorie sweetener with high sweetness, faces production challenges due to its low yield and purity. UGT76G1, a uridine diphosphate glucose (UDPG)-dependent glycosyltransferase, forms a β-1,3-glycosidic bond with rebaudioside D (Reb D) to produce Reb M but with an efficiency lower than that for stevioside (ST).
Fangwei Song +7 more
openaire +2 more sources