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Raman Spectroscopic Analysis of Steviol Glycosides: Spectral Database and Quality Control Algorithms. [PDF]
FoodsPezzotti G +6 more
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Gut Microbiota Composition in Rats Consuming Sucralose or Rebaudioside A at Recommended Doses Under Two Dietary Interventions. [PDF]
MetabolitesRamos-García M +10 more
europepmc +1 more source
LED light improves shoot multiplication, steviol glycosides and phenolic compounds biosynthesis in Stevia rebaudiana Bertoni in vitro culture. [PDF]
Sci RepPtak A +4 more
europepmc +1 more source
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Plant Physiology and Biochemistry, 2021
Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S.
Heng Yin, Xiaochen Jia, Heng Yin
exaly +3 more sources
Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S.
Heng Yin, Xiaochen Jia, Heng Yin
exaly +3 more sources
International Journal of Biological Macromolecules, 2020
Rebaudioside D is a promising sweetener due to its zero calorie and high sweetness. Here, a transglucosylase gene eugt11 from Oryza sativa was for the first time expressed in Pichia pastoris, and transformant XE-3 showed the highest expression levels in pH 5.5 BMMY media containing 0.75% methanol.
Hao Song, Tong Huang, Jiefang Hong
exaly +3 more sources
Rebaudioside D is a promising sweetener due to its zero calorie and high sweetness. Here, a transglucosylase gene eugt11 from Oryza sativa was for the first time expressed in Pichia pastoris, and transformant XE-3 showed the highest expression levels in pH 5.5 BMMY media containing 0.75% methanol.
Hao Song, Tong Huang, Jiefang Hong
exaly +3 more sources
Journal of Agricultural and Food Chemistry, 2019
In this study, a monoglucosyl rebaudioside A product was isolated from the mixture of glucosylated rebaudioside A obtained from the most reported and industrial used cyclodextrin glycosyl transferase, Toruzyme 3.0 L (CGTase, Toruzyme 3.0 L). The molecular structure of the monoglucosyl rebaudioside A was characterized using LC-MS/MS and methylation ...
Tongtong Zhang +2 more
exaly +3 more sources
In this study, a monoglucosyl rebaudioside A product was isolated from the mixture of glucosylated rebaudioside A obtained from the most reported and industrial used cyclodextrin glycosyl transferase, Toruzyme 3.0 L (CGTase, Toruzyme 3.0 L). The molecular structure of the monoglucosyl rebaudioside A was characterized using LC-MS/MS and methylation ...
Tongtong Zhang +2 more
exaly +3 more sources
Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 and in situ UDP-glucose regeneration
Food Chemistry, 2018Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4-0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside.
Kequan Chen, , Liangliang Chen
exaly +3 more sources
Regulatory Toxicology and Pharmacology, 2014
The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd, Purkayastha +5 more
openaire +2 more sources
The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd, Purkayastha +5 more
openaire +2 more sources
Biochemical Engineering Journal, 2020
Abstract Engineering of enzymes without crystal structures and homologous structures is a great challenge for structure-based computational enzyme design. In this study, a computational strategy that combines protein structure prediction, sequence optimization, and molecular dynamics simulation was developed to improve the catalytic efficiency of the
Min Lin, Fei Wang, Yushan Zhu
openaire +1 more source
Abstract Engineering of enzymes without crystal structures and homologous structures is a great challenge for structure-based computational enzyme design. In this study, a computational strategy that combines protein structure prediction, sequence optimization, and molecular dynamics simulation was developed to improve the catalytic efficiency of the
Min Lin, Fei Wang, Yushan Zhu
openaire +1 more source