Single Cell T Cell Receptor Sequencing: Techniques and Future Challenges
Frontiers in Immunology, 2018 The peculiarity of T cell is their ability to recognize an infinite range of self and foreign antigens. This ability is achieved during thymic development through a complex molecular mechanism based on somatic recombination that leads to the expression ...
Marco De Simone +3 more
doaj +1 more source
Alpha/beta T-cell antigen receptor gene and protein expression occurs at early stages of thymocyte differentiation. [PDF]
Proceedings of the National Academy of Sciences, 1988 Alterations in gene expression that orchestrate eukaryotic cellular differentiation often require appropriate interactions between differentiating cells and a specialized microenvironment. During T-lymphocyte differentiation, immature thymocytes undergo a stringent intrathymic selection process that requires intimate contact with thymic stromal ...
E R, Richie +5 more
openaire +2 more sources
Soluble T-cell receptors produced in human cells for targeted delivery. [PDF]
PLoS ONE, 2015 Recently, technology has become available to generate soluble T-cell receptors (sTCRs) that contain the antigen recognition part. In contrast to antibodies, sTCRs recognize intracellular in addition to extracellular epitopes, potentially increasing the ...
Even Walseng +6 more
doaj +1 more source
Intestinal αβ T Cells Differentiate and Rearrange Antigen Receptor Genes In Situ in the Human Infant [PDF]
The Journal of Immunology, 2004 Abstract Intestinal Ag exposure during neonatal life influences appropriate adult immune responses. To define the mechanisms shaping the T cell repertoire during this period, we examined T cell differentiation and receptor diversity in the intestine of human infants.
Williams, AM +7 more
openaire +2 more sources
Expansion of natural (NK1+) T cells that express alpha beta T cell receptors in transporters associated with antigen presentation-1 null and thymus leukemia antigen positive mice. [PDF]
The Journal of experimental medicine, 1996 Thymic selection of natural killer-1+ natural T cells that express alpha beta T cell receptors requires a conserved beta 2-microglobulin-associated molecule, presumably CD1d, displayed by CD4+8+ thymocytes. Here we demonstrate that positive selection of natural T cells occurs independent of transporters associated with antigen presentation-1 (TAP-1 ...
S, Joyce +7 more
openaire +2 more sources
Can We Improve Vaccine Efficacy by Targeting T and B Cell Repertoire Convergence?
Frontiers in Immunology, 2019 Traditional vaccine development builds on the assumption that healthy individuals have virtually unlimited antigen recognition repertoires of receptors in B cells and T cells [the B cell receptor (BCR) and TCR respectively].
Katja Fink
doaj +1 more source
Structural and biophysical insights into the role of CD4 and CD8 in T cell activation
Frontiers in Immunology, 2013 T cell receptors (TCRs) recognize peptides presented by MHC molecules (pMHC) on an antigen-presenting cell (APC) to discriminate foreign from self antigens and initiate adaptive immune responses.
Yili eLi, Roy eMariuzza
doaj +1 more source
Distinctive CD3 Heterodimeric Ectodomain Topologies Maximize Antigen-Triggered Activation of αβ T Cell Receptors [PDF]
The Journal of Immunology, 2010 Abstract The αβ TCR has recently been suggested to function as an anisotropic mechanosensor during immune surveillance, converting mechanical energy into a biochemical signal upon specific peptide/MHC ligation of the αβ clonotype. The heterodimeric CD3εγ and CD3εδ subunits, each composed of two Ig-like ectodomains, form unique side-to ...
Sun Taek, Kim +7 more
openaire +2 more sources
Developmental arrest of NK1.1+ T cell antigen receptor (TCR)-alpha/beta+ T cells and expansion of NK1.1+ TCR-gamma/delta+ T cell development in CD3 zeta-deficient mice. [PDF]
The Journal of experimental medicine, 1995 The relationship between the structure of the T cell antigen receptor (TCR)-CD3 complex and development of NK1.1+ T cells was investigated. The TCR complex of freshly isolated NK1.1+ TCR-alpha/beta+ thymocytes contained CD3 zeta homodimers and CD zeta-FcR gamma heterodimers, whereas that of the majority of NK1.1- T cells did not contain FcR gamma.
H, Arase +7 more
openaire +2 more sources
Evidence that the T cell antigen receptor may not be involved in cytotoxicity mediated by gamma/delta and alpha/beta thymic cell lines. [PDF]
The Journal of experimental medicine, 1987 After culture in IL-2, thymocytes expressing either TCR-alpha/beta or -gamma/delta acquired the ability to lyse hematopoietic and solid tumor cell targets without deliberate immunization or apparent restriction by the MHC. Moreover, TCR-alpha/beta- and TCR-gamma/delta-bearing thymic cell lines demonstrated an essentially identical spectrum of cytolysis
Phillips, JH +4 more
openaire +4 more sources