Results 101 to 110 of about 13,061,353 (246)

Prevalence of methicillin-resistant Staphylococcus aureus isolated from Moheb Hospital and Miladphenotypic and molecular methods

Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, 2015
Introduction: S. aureus, the second most common cause of nosocomial infections, is regarded as an important factor in the severe infections of the community. Methicillin-resistant strains of this bacterium involve a major pathogen which can cause disease
Z Gomarian, MH Shahhosseiny, M Bayat, MA Mahmoudi, T Nafarieh, M Rahbar   +5 more
doaj  

Identification and Evaluation of qRT-PCR Reference Genes in Melanaphis sacchari

Insects
Appropriate reference genes must be selected for accurate qRT-PCR data to conduct a thorough gene expression analysis in the sorghum aphid (Melanaphis sacchari, Hemiptera, Aphididae). This approach will establish a foundation for gene expression analysis
Kunliang Zou, Tonghan Wang, Minghui Guan, Yang Liu, Jieqin Li, Yanlong Liu, Junli Du, Degong Wu   +7 more
doaj   +1 more source

Creating reference gene annotation for the mouse C57BL6/J genome assembly

Mammalian Genome, 2015
Annotation on the reference genome of the C57BL6/J mouse has been an ongoing project ever since the draft genome was first published. Initially, the principle focus was on the identification of all protein-coding genes, although today the importance of ...
Jonathan M. Mudge, J. Harrow
semanticscholar   +1 more source

Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants [PDF]

, 1985
The antibiotic G418 was shown to be an effective inhibitor of vaccinia virus replication when an appropriate concentration of it was added to cell monolayers 48 h before infection. Genetic engineering techniques were used in concert with DNA transfection
Franke, Christine A., Hruby, Dennis E., Rice, Charles M., Strauss, James H.   +3 more
core  

Regression modeling on stratified data with the lasso

, 2016
We consider the estimation of regression models on strata defined using a categorical covariate, in order to identify interactions between this categorical covariate and the other predictors.
Ollier, Edouard, Viallon, Vivian
core   +3 more sources

Identification of a Novel Reference Gene for Apple Transcriptional Profiling under Postharvest Conditions

PLoS ONE, 2015
Reverse Transcription quantitative PCR (RT-qPCR) is one of the most important techniques for gene expression profiling due to its high sensibility and reproducibility.
Tatiane Timm Storch, C. Pegoraro, Taciane Finatto, V. Quecini, C. Rombaldi, C. L. Girardi   +5 more
semanticscholar   +1 more source

Selection of Reference Genes for Expression Normalization by RT-qPCR in Dracocephalum moldavica L.

Current Issues in Molecular Biology
Dracocephalum moldavica is widely used as an ornamental, medicine, and perfume in industry. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) is widely and accurately utilized for gene expression evaluations.
Shasha Li, Xiaomin Ge, Guoqing Bai, Chen Chen   +3 more
doaj   +1 more source

Selection of Reliable Reference Genes for Gene Expression Studies on Rhododendron molle G. Don

Frontiers in Plant Science, 2016
The quantitative real-time polymerase chain reaction (qRT-PCR) approach has become a widely used method to analyze expression patterns of target genes.
Zheng Xiao, Xiaobo Sun, Xiaoqing Liu, Chang Li, Lisi He, Shangping Chen, Jiale Su   +6 more
doaj   +1 more source

Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers [PDF]

, 2011
Background: Gene regulation through cis-regulatory elements plays a crucial role in development and disease. A major aim of the post-genomic era is to be able to read the function of cis-regulatory elements through scrutiny of their DNA sequence ...
Bronner, Marianne E., Elgar, Greg, Parker, Hugo J., Piccinelli, Paul, Sauka-Spengler, Tatjana   +4 more
core   +5 more sources

Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage

Nature Immunology, 2018
Tissue fibrosis is a major cause of mortality that results from the deposition of matrix proteins by an activated mesenchyme. Macrophages accumulate in fibrosis, but the role of specific subgroups in supporting fibrogenesis has not been investigated in ...
D. Aran, Agnieszka P. Looney, Leqian Liu, Esther Wu, Valerie Fong, Austin Hsu, S. Chak, Ram P. Naikawadi, Paul J. Wolters, A. Abate, A. Butte, M. Bhattacharya   +11 more
semanticscholar   +1 more source