Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Reference Gene Stability Values in Validation Set.
, 2016 Reference Gene Stability Values in Validation Set.
Jack L. Martin (3154944)
core +1 more source
Tissue-specific validation of reference genes for accurate qRT-PCR analysis during sex differentiation in Amphiprion ocellaris [PDF]
Brazilian Journal of BiologyQuantitative real-time PCR (qRT-PCR) is a widely used method for measuring gene expression, but its reliability depends on using stable reference genes for normalization.
C. Boonphakdee +3 more
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Protein sequence of the reference gene catalog
, 2022 A fasta file of reference gene catalog of golden snub-nosed monkey. It contains the protein sequence of 5,986,337 non-redundant genes.
Du Zhang (12882473)
core +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Reference genes in aging: what are you referring to?
Aging, 2023 Mishra, Manish, Howlett, Susan E.
openaire +2 more sources
Influence of reference genes on expression of TYR, MITF and ASIP genes in tissues of Silky Fowl
浙江大学学报. 农业与生命科学版, 2015 Silky Fowl is a precious local breed containing a large amount of melanin. Therefore, it is considered to have high edible and medicinal value. Study on the related gene expression is helpful to reveal the internal regulation mechanism of melanin ...
Zheng Nenzhu +7 more
doaj +1 more source