Results 121 to 130 of about 13,061,353 (246)

Selection of reference genes in canine uterine tissues

Genetics and Molecular Research, 2016
Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by ...
M, Du, X, Wang, Y W, Yue, P Y, Zhou, W, Yao, X, Li, X B, Ding, X F, Liu, H, Guo, W Z, Ma   +9 more
openaire   +2 more sources

Probabilities of spurious connections in gene networks: Application to expression time series

, 2004
Motivation: The reconstruction of gene networks from gene expression microarrays is gaining popularity as methods improve and as more data become available.
Bickel, D. R. Bickel, de Jong, Efron, Fernando, Pepe   +5 more
core   +1 more source

Correction to: Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]

BMC Genomics, 2021
dos Santos, K. C. G., Desgagné-Penix, I., Germain, H.   +2 more
openaire   +3 more sources

Expression of Nitrilases in Brassica juncea var. tumida Tsen in Root Galls Caused by Plasmodiophora brassicae

Journal of Integrative Agriculture, 2012
Five commonly-used reference genes: ACT (actin), UBE (ubiquitin-conjugating enzyme), RPL2 (ribosomal protein L2), BRP II (RNA polymerase II subunit), and NADH (nicotinamide adenine dinucleotide) were examined using geNorm software as reference genes for ...
Yan LIU, You-ping YIN, Zhong-kang WANG, Yuan-li LUO   +3 more
doaj   +1 more source

Expression Profiling in Bemisia tabaci under Insecticide Treatment: Indicating the Necessity for Custom Reference Gene Selection

PLoS ONE, 2014
Finding a suitable reference gene is the key for qRT-PCR analysis. However, none of the reference gene discovered thus far can be utilized universally under various biotic and abiotic experimental conditions.
P. Liang, Ya-li Guo, Xuguo Zhou, Xiwu Gao   +3 more
semanticscholar   +1 more source

Tissue-specific validation of reference genes for accurate qRT-PCR analysis during sex differentiation in Amphiprion ocellaris [PDF]

Brazilian Journal of Biology
Quantitative real-time PCR (qRT-PCR) is a widely used method for measuring gene expression, but its reliability depends on using stable reference genes for normalization.
C. Boonphakdee, J. Thamnawasolos, T. Boonphakdee, T. Mataroepayotorn   +3 more
doaj   +1 more source

Reliable reference gene selection for Cordyceps militaris gene expression studies under different developmental stages and media.

FEMS Microbiology Letters, 2014
Cordyceps militaris is considered a model organism for the study of Cordyceps species, which are highly prized in traditional Chinese medicine. Gene expression analysis has become more popular and important in studies of this fungus.
Tiantian Lian, T. Yang, Guijun Liu, Junde Sun, C. Dong   +4 more
semanticscholar   +1 more source

Reference Standards for Gene and Cell Therapy Products [PDF]

Molecular Therapy, 2017
Reference standards are important tools for the calibration of medical products and procedures. They are particularly important when dosing and potency are critical parameters for patient safety and product efficacy.1 Reference standards benefit the field by fostering best practices for the manufacturing and testing of safe and efficacious medicinal ...
openaire   +2 more sources

Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells

Nanotechnology, Science and Applications, 2020
Rajendran K Selvakesavan, Gregory Franklin Institute of Plant Genetics of the Polish Academy of Sciences, Poznan 60-479, PolandCorrespondence: Gregory Franklin Tel +48 61 655 0266Email fgre@igr.poznan.plPurpose: We report on the expression stability of ...
Selvakesavan RK, Franklin G
doaj  

The choice of reference gene affects statistical efficiency in quantitative PCR data analysis

BioTechniques, 2013
Quantitative polymerase chain reaction (qPCR), a highly sensitive method of measuring gene expression, is widely used in biomedical research.
Yi Guo, Michael L. Pennell, Dennis K. Pearl, Thomas J. Knobloch, Soledad Fernandez, Christopher M. Weghorst   +5 more
doaj   +1 more source