Results 271 to 280 of about 8,117,731 (311)

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Exploring transcriptomic databases to identify and experimentally validate tissue-specific consensus reference gene for gene expression normalization in BALB/c mice acutely exposed to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin. [PDF]

Curr Res Toxicol
Hammoudeh N, Hasan R, Deeb M, Radwan Z, Ayoubi O, Alendary R, Youssef M, Kazan A, Alsahli R, Faiad W, Aldeli N, Hanano A.   +11 more
europepmc   +1 more source

Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L. [PDF]

Sci Rep
Vijayakumar S, Sakuntala M.
europepmc   +1 more source

Identification of specific reference gene for normalization of RT-qPCR data in rhythmic gene expression studies of the effect of developmental hormone antagonist in postembryonic development in Bombyx mori. [PDF]

Front Insect Sci
Dalai M, Jagota A.
europepmc   +1 more source

Reference Genes in Real-Time PCR [PDF]

Journal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Marcin Rapacz
exaly   +3 more sources

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Selection of reference genes for gene expression studies in astrocytomas

Analytical Biochemistry, 2011
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene.
Sylwia M, Gresner, Ewa, Golanska, Dominika, Kulczycka-Wojdala, Dariusz J, Jaskolski, Wielislaw, Papierz, Pawel P, Liberski   +5 more
openaire   +2 more sources

Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis

2021
Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference gene is critically important for gene expression analysis due to that wrong reference genes will cause misleading and even wrong conclusion. A good
Xiaoping, Pan, Baohong, Zhang
openaire   +2 more sources

Validation of reference genes in the feline endometrium

Reproductive Biology, 2014
The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).
Ewelina, Jursza, Dariusz J, Skarzynski, Marta J, Siemieniuch   +2 more
openaire   +2 more sources

Candidate reference genes for gene expression studies in water lily

Analytical Biochemistry, 2010
The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder.
Huolin, Luo, Sumei, Chen, Hongjian, Wan, Fadi, Chen, Chunsun, Gu, Zhaolei, Liu   +5 more
openaire   +2 more sources

A reference gene set for chemosensory receptor genes of Manduca sexta

Insect Biochemistry and Molecular Biology, 2015
The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context ...
Koenig, C., Hirsh, A., Bucks, S., Klinner, C., Vogel, H., Shukla, A., Mansfield, J., Morton, B., Hansson, B., Grosse-Wilde, E.   +9 more
openaire   +3 more sources