Results 281 to 290 of about 8,117,731 (311)

Rice Reference Genes: redefining reference genes in rice by mining RNA-seq datasets

Plant and Cell Physiology
Abstract Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression.
Xin Liu, Siyuan Tang, Yingbo Gao, Xiaoxiang Zhang, Guichun Dong, Juan Zhou, Yong Zhou, Zefeng Yang, Jianye Huang, Youli Yao   +9 more
openaire   +2 more sources

Identification of suitable reference genes in the mouse placenta

Placenta, 2016
Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a reliable tool to analyse gene expression profiles. The expression of housekeeping genes generally serves as a reference for mRNA amount, assuming that it remains stable under pathophysiological and experimental conditions. To date, an empirical validation of reference
María Emilia Solano, Kristin Thiele, Mirka Katharina Kowal, Petra Clara Arck   +3 more
openaire   +2 more sources

Evaluation of candidate reference genes for qPCR in maize

Journal of Plant Physiology, 2012
Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels. Accurate and reproducible results are dependent on the correct choice of the reference genes for data normalization. To date, screenings evaluating candidate reference gene stability for expression studies in maize have not been reported.
MANOLI, ALESSANDRO, STURARO, ALBA, TREVISAN, SARA, QUAGGIOTTI, SILVIA, NONIS, ALBERTO   +4 more
openaire   +3 more sources

Selection of reliable reference genes for gene expression studies in Botrytis cinerea

Journal of Microbiological Methods, 2017
Botrytis cinerea is an important plant pathogen causing grey mold disease in a wide range of plant species. The aim of this study was to identify reliable reference genes that can be used for the analysis of relative gene expression in B. cinerea with quantitative real-time reverse transcription PCR (qRT-PCR).
He, Ren, Xiaoqing, Wu, Yuping, Lyu, Hongzi, Zhou, Xueying, Xie, Xinjian, Zhang, Hetong, Yang   +6 more
openaire   +2 more sources

Evaluation of candidate reference genes in Clostridium difficile for gene expression normalization

Anaerobe, 2010
Quantitative real-time polymerase chain reaction (qPCR) is a sensitive, efficient and reproducible technique for studying gene expression. Identification of stably expressed reference genes is required to avoid bias in these studies yet mostly unvalidated reference genes are used in studying gene expression in Clostridium difficile.
Devon, Metcalf, Shayan, Sharif, J Scott, Weese   +2 more
openaire   +2 more sources

Validation of reference genes for quantitative measurement of immune gene expression in shrimp

Molecular Immunology, 2009
To accurately measure the relative expression of a target gene, mRNA expression data is routinely normalized with reference to an internal control gene. We examined the transcriptional stability of four internal control genes, beta-actin, glyceraldehyde-3 phosphate dehydrogenase (GAPDH), elongation factor1-alpha (EF1-alpha), and 18S ribosomal RNA (18S ...
Arun K, Dhar, Robert M, Bowers, Kate S, Licon, Gregory, Veazey, Betsy, Read   +4 more
openaire   +2 more sources

Reference genes to quantify gene expression during oogenesis in a teleost fish

Gene, 2012
Understanding the molecular events involved in the acquisition of competence during oogenesis is a key step to determine the secret of 'high quality' eggs for aquaculture. Quantitative real time polymerase chain reaction (qPCR) is the technique of election to determine changes in transcript abundance in such studies, but choosing reference genes for ...
Laurence A M, Deloffre, André, Andrade, Alexandra I, Filipe, Adelino V M, Canario   +3 more
openaire   +2 more sources

Reference genes for accurate normalization of gene expression in wood-decomposing fungi

Fungal Genetics and Biology, 2019
Wood-decomposing fungi efficiently decompose plant lignocellulose, and there is increasing interest in characterizing and perhaps harnessing the fungal gene regulation strategies that enable wood decomposition. Proper interpretation of these fungal mechanisms relies on accurate quantification of gene expression, demanding reliable internal control ...
Jiwei, Zhang, Hugh D, Mitchell, Lye Meng, Markillie, Matthew J, Gaffrey, Galya, Orr, Jonathan, Schilling   +5 more
openaire   +2 more sources

Validation of Reference Genes in Chordoma

Journal of Neurological Surgery Part B: Skull Base, 2014
Remco Santegoeds, Y. Yakkioui, J. Van Overbeeke, Y. Temel   +3 more
openaire   +1 more source

RefFinder: A Web-Based Tool for Comprehensively Analyzing and Identifying Reference Genes

Functional and Integrative Genomics, 2023
Baohong Zhang, Fuliang Xie
exaly