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A reference gene set for chemosensory receptor genes of Manduca sexta
Insect Biochemistry and Molecular Biology, 2015The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context ...
Koenig, C. +9 more
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Selection of reliable reference genes for gene expression studies in Botrytis cinerea
Journal of Microbiological Methods, 2017Botrytis cinerea is an important plant pathogen causing grey mold disease in a wide range of plant species. The aim of this study was to identify reliable reference genes that can be used for the analysis of relative gene expression in B. cinerea with quantitative real-time reverse transcription PCR (qRT-PCR).
Xinjian Zhang +2 more
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Rice Reference Genes: redefining reference genes in rice by mining RNA-seq datasets
Plant and Cell PhysiologyAbstract Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression.
Xin Liu +9 more
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Identification of suitable reference genes in the mouse placenta
Placenta, 2016Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a reliable tool to analyse gene expression profiles. The expression of housekeeping genes generally serves as a reference for mRNA amount, assuming that it remains stable under pathophysiological and experimental conditions. To date, an empirical validation of reference
María Emilia Solano +3 more
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Evaluation of candidate reference genes for qPCR in maize
Journal of Plant Physiology, 2012Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels. Accurate and reproducible results are dependent on the correct choice of the reference genes for data normalization. To date, screenings evaluating candidate reference gene stability for expression studies in maize have not been reported.
MANOLI, ALESSANDRO +4 more
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Validation of reference genes for quantitative measurement of immune gene expression in shrimp
Molecular Immunology, 2009To accurately measure the relative expression of a target gene, mRNA expression data is routinely normalized with reference to an internal control gene. We examined the transcriptional stability of four internal control genes, beta-actin, glyceraldehyde-3 phosphate dehydrogenase (GAPDH), elongation factor1-alpha (EF1-alpha), and 18S ribosomal RNA (18S ...
Robert Bowers, Arun Dhar
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Reference genes for accurate normalization of gene expression in wood-decomposing fungi
Fungal Genetics and Biology, 2019Wood-decomposing fungi efficiently decompose plant lignocellulose, and there is increasing interest in characterizing and perhaps harnessing the fungal gene regulation strategies that enable wood decomposition. Proper interpretation of these fungal mechanisms relies on accurate quantification of gene expression, demanding reliable internal control ...
Jiwei, Zhang +5 more
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Validation of Reference Genes in Chordoma
Journal of Neurological Surgery Part B: Skull Base, 2014Remco Santegoeds +3 more
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