Results 51 to 60 of about 2,306,220 (297)
Approach for defining endogenous reference genes in gene expression experiments
Analytical Biochemistry, 2004 The quantification of gene expression by real-time polymerase chain reaction (PCR) has revolutionized the field of gene expression analysis. Due to its sensitivity and flexibility it is becoming the method of choice for many investigators. However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
Garcia Vallejo, J.J. +6 more
openaire +3 more sources
ERgene: Python library for screening endogenous reference genes [PDF]
Scientific Reports, 2020 AbstractIn gene expression analysis, sample differences and experimental operation differences are common, but sometimes, these differences will cause serious errors to the results or even make the results meaningless. Finding suitable internal reference genes efficiently to eliminate errors is a challenge.
Zeng, Zehua +3 more
openaire +2 more sources
The Sorghum bicolor reference genome: improved assembly, gene annotations, a transcriptome atlas, and signatures of genome organization. [PDF]
, 2017 Sorghum bicolor is a drought tolerant C4 grass used for the production of grain, forage, sugar, and lignocellulosic biomass and a genetic model for C4 grasses due to its relatively small genome (approximately 800 Mbp), diploid genetics, diverse ...
Amirebrahimi, Mojgan +14 more
core +2 more sources
Frontiers in Physiology, 2018 Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Jing Lü +7 more
doaj +1 more source
VaccinesBackground/Objectives: The SARS-CoV-2 pandemic challenged patients with inflammatory bowel disease (IBD) under immunosuppressive therapies. We used data from the RisCoin cohort to investigate factors associated with a poor immune response to mRNA ...
Katarina Csollarova +14 more
doaj +1 more source
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
An alternative to Kitcher's theory of conceptual progress and his account of the change of the gene concept [PDF]
, 2004 The present paper discusses Kitcher’s framework for studying conceptual change and progress. Kitcher’s core notion of reference potential is hard to apply to concrete cases.
Brigandt, Ingo
core +1 more source
Cerebellar Cortex, Purkinje Cell Layer [PDF]
, 2008 This report contains a summary of expression patterns for genes that are enriched in the Purkinje cell layer (CBXpu) of the cerebellum. All data is derived from the Allen Brain Atlas (ABA) in situ hybridization mouse project.
Allen Institute for Brain Science +3 more
core +2 more sources
Validation of reference genes in human chordoma
Surgical Neurology International, 2017 Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C +5 more
openaire +3 more sources
Sickle Cell Disease Is an Inherent Risk for Asthma in a Sibling Comparison Study
Pediatric Blood &Cancer, EarlyView.ABSTRACT Introduction Sickle cell disease (SCD) and asthma share a complex relationship. Although estimates vary, asthma prevalence in children with SCD is believed to be comparable to or higher than the general population. Determining whether SCD confers an increased risk for asthma remains challenging due to overlapping symptoms and the ...
Suhei C. Zuleta De Bernardis +9 more
wiley +1 more source