Results 221 to 230 of about 264,833 (258)

PIAS Proteins as Repressors of Oct4 Function

Journal of Molecular Biology, 2007
The POU domain transcription factor Oct4 plays essential functions in the maintenance of pluripotent embryonic and germ cells of mammals. Molecular mechanisms of Oct4 action remain poorly understood. To isolate modulators of Oct4 activity, we performed a yeast two-hybrid screen with the Oct4 POU domain as a bait and isolated PIASy as an Oct4 ...
Tolkunova, E., Malashicheva, A., Parfenov, V., Sustmann, C., Grosschedl, R., Tomilin, A.   +5 more
openaire   +4 more sources

Plasmid-mediated isolation of a repressor protein

Analytical Biochemistry, 1980
Abstract A multicopy chimeric plasmid enriched for operator sequences has been used to mobilize and isolate a repressor protein. The procedure relies on the intact isolation of a plasmid DNA-protein complex, precipitation of the complex with polyethylene glycol, ionic elution of the bound protein, and final purification on phosphocellulose columns ...
Raju K. Koduri, J T Matthews, Joseph S. Gots   +2 more
openaire   +3 more sources

Regulation in repressor inactivation by RecA protein

Advances in Biophysics, 1990
Treatments that damage DNA or inhibit DNA synthesis in E. coli induce the expression of a set of functions called SOS functions that are involved in DNA repair, mutagenesis, arrest of cell division and prophage induction. Induction of SOS functions is triggered by inactivation of the LexA repressor or a phage repressor. Inactivation of these repressors
Hideyuki Ogawa, Tomoko Ogawa
openaire   +3 more sources

Ultraviolet difference spectra of the lactose repressor protein

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1973
Abstract Ultraviolet difference spectra of the lactose repressor have been studied under various conditions. Changes in the aromatic residues of the protein upon addition of inducers, but not anti-inducers, are found. The extent of exposure of aromatic residues to solvent perturbation is also found to differ in the presence of inducers and anti ...
H. R. Matthews, Oleg Jardetzky, Kathleen S. Matthews, H. W. Thielmann   +3 more
openaire   +3 more sources

Strigolactone Signaling: Repressor Proteins Are Transcription Factors

Trends in Plant Science, 2020
A recent landmark study by Wang et al. provides new insight into transcriptional regulation in strigolactone (SL) signaling. The finding that SUPPRESSOR OF MAX2 LIKE 6 (SMXL6) also functions as an autoregulated transcription factor (TF) causes a paradigm shift in the current view of transcriptional repressors in phytohormone signaling.
Jiuyou Tang, Chengcai Chu
openaire   +3 more sources

Modification of tyrosine residues of the lactose repressor protein

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1977
Reaction of the lactose repressor protein from Escherichia coli with high molar excesses (up to 800 fold) of tetranitromethane resulted in modification of tyrosine residues in the amino-terminal and core regions of the molecule. Tyrosines 7 and 17 exhibit significant reactivity at low levels (5-10 fold molar excess) of tetranitromethane.
Kathleen S. Matthews, M.D. Shaw, Alex A. Burgum, M.E. Alexander, R.A. Noall   +4 more
openaire   +3 more sources

Lac repressor is a transient gene-activating protein

Cell, 1987
We show, using a combination of methods, that contrary to the usual view, lac repressor increases, by more than 100-fold, the initial binding of RNA polymerase to E. coli lac UV5 promoter DNA. Kinetic studies revealed that the repressor acts to block the isomerization step in transcription initiation.
Susan B. Straney, Donald M. Crothers
openaire   +3 more sources

Thermal denaturation of the core protein of lac repressor

Biochemistry, 1985
The thermal denaturation of the core protein of lac repressor was studied alone and in the presence of the inducer isopropyl beta-D-thiogalactoside (IPTG) and the antiinducer o-nitrophenyl beta-D-fucoside (ONPF) by means of high-sensitivity differential scanning calorimetry.
Susan P. Manly, Kathleen S. Matthews, Julian M. Sturtevant   +2 more
openaire   +3 more sources

Escherichia coli Repressor Proteins

1989
Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
openaire   +2 more sources

Immobilisation of a repressor protein for binding of plasmid DNA

Journal of Chromatography A, 2005
The use of plasmid DNA in gene therapy and genetic vaccination has increased the need for scalable and sustainable production processes. One key challenge for bioprocess engineering is the separation of plasmid DNA from structurally related impurities. Affinity purification procedures allow a highly selective capturing of the target molecule.
Anja Hasche, Carsten Voß
openaire   +3 more sources