Results 21 to 30 of about 54,681 (257)

Retinal Pigment Epithelial Cells Produce Fibronectin

Ophthalmic Research, 1987
Antichick fibronectin antiserum, noncross-reactive to bovine fibronectin, was prepared to determine the production of fibronectin by cultured chick retinal pigment epithelial cells which were grown in the presence of fetal bovine serum. The typical fibrillary net pattern of fibronectin was observed by an indirect immunofluorescent technique when this ...
R, Yamakawa, H, Shirakawa, M, Okada, N, Yoshimura, M, Matsumura, N, Ogino   +5 more
openaire   +2 more sources

Sustained gene expression in the retina by improved episomal vectors [PDF]

, 2014
Gene and cellular therapies are nowadays part of therapeutic strategies for the treatment of diverse pathologies. The drawbacks associated with gene therapy-low levels of transgene expression, vector loss during mitosis, and gene silencing-need to be ...
Ana V. Oliveira, Conley S.M., Davis A.A., Gabriela A. Silva, Reinisalo M., Rudolf Haase, Sofia M. Calado, Susana Machado   +7 more
core   +1 more source

Electrical estimulation of retinal pigment epithelial cells

Experimental Eye Research, 2010
We investigated and characterized the effect of externally applied electric fields (EF) on retinal pigment epithelial (RPE) cells by exposing primary cultures of human RPE cells (hRPE) and those from the ARPE19 immortalized cell line to various strengths of EF (EF-treated cells) or to no EF (control cells) under different conditions including presence ...
Gamboa, O.L., Forrester, J.V., Lois, N., Pu, J., McCaig, C., Townend, J., Zhao, M.   +6 more
openaire   +4 more sources

Preclinical Evaluation of a Cell-Based Gene Therapy Using the Sleeping Beauty Transposon System in Choroidal Neovascularization

Molecular Therapy: Methods & Clinical Development, 2019
Age-related macular degeneration (AMD) is a progressive retinal disorder characterized by imbalanced pro- and antiangiogenic signals. The aim of this study was to evaluate the effect of ex vivo cell-based gene therapy with stable expression of human ...
Maria Hernandez, Sergio Recalde, Laura Garcia-Garcia, Jaione Bezunartea, Csaba Miskey, Sandra Johnen, Sabine Diarra, Attila Sebe, Juan Roberto Rodriguez-Madoz, Severine Pouillot, Corinne Marie, Zsuzsanna Izsvák, Daniel Scherman, Martina Kropp, Felipe Prosper, Gabriele Thumann, Zoltán Ivics, Alfredo Garcia-Layana, Patricia Fernandez-Robredo   +18 more
doaj   +1 more source

A defined subset of clonal retinal stem cell spheres is biased to RPE differentiation

iScience, 2021
Summary: Retinal stem cells (RSCs) are rare pigmented cells found in the pigmented ciliary layer of the mammalian retina. Studies show that RSCs can replicate to maintain the stem cell pool and produce retinal progenitors that differentiate into all ...
Tahani W. Baakdhah, Brenda Coles, Derek van der Kooy   +2 more
doaj   +1 more source

Profound Re-Organization of Cell Surface Proteome in Equine Retinal Pigment Epithelial Cells in Response to In Vitro Culturing [PDF]

, 2012
The purpose of this study was to characterize the cell surface proteome of native compared to cultured equine retinal pigment epithelium (RPE) cells. The RPE plays an essential role in visual function and represents the outer blood-retinal barrier.
Aisenbrey, Alcazar, Alge, Bhutto, Bodaghi, Burke, Campochiaro, Christoph Szober, Chu, Claudia Alge-Priglinger, Clegg, Cornelia Deeg, Deeg, Deeg, Deeg, Deeg, Deeg, Degroote, Doherty, Dunn, Eberhardt, Ehrenhofer, Greer, Gullapalli, Gundersen, Ham, Hamel, Hauck, Hauck, Hauck, Hauck, Ho, Huang, Karl, Kerstin Euler, Kristina Fröhlich, Li, Limb, Machemer, Marius Ueffing, McBee, Milyushina, Mochizuki, Muramatsu, Nagai, Parker, Philp, Rizzolo, Rotilio, Singer, Stefanie Hauck, Strauss, Vacanti, Wolf, Wu   +54 more
core   +3 more sources

Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations

Journal of Tissue Engineering, 2016
The retinal pigment epithelium is an important part of the vertebrate eye, particularly in studying the causes and possible treatment of age-related macular degeneration.
Aaron H Fronk, Elizabeth Vargis
doaj   +1 more source

Hydrogen peroxide enhances transforming growth factor beta-2 induced epithelial–mesenchymal transition of ARPE-19 cells

Beni-Suef University Journal of Basic and Applied Sciences, 2023
Background In the pathophysiology of the retina, the epithelial–mesenchymal transition and oxidative stress play key roles. To find a relationship between oxidative stress and diabetic retinopathy or proliferative retinopathy, we used an in-silico ...
Brijesh Gelat, Pooja Rathaur, Pooja Malaviya, Binita Patel, Abhay R. Vasavada, S. R. Kaid Johar   +5 more
doaj   +1 more source

Early stages of retinal development depend on Sec13 function [PDF]

, 2013
Summary ER-to-Golgi transport of proteins destined for the extracellular space or intracellular compartments depends on the COPII vesicle coat and is constitutive in all translationally active cells.
Cavodeassi, Florencia, Feng, Yi, Schmidt, Katy, Stephens, David J   +3 more
core   +3 more sources

Retinal Pigment Epithelial Cells Release Inhibitors of Neovascularization

Ophthalmology, 1987
Human retinal pigment epithelial (RPE) cells in culture were found to release a substance (or substances) that causes the regression of new blood vessels on the chick embryonic yolk sac and inhibits proliferation of fetal bovine aortic endothelial cells and human retinal microvessel endothelial cells in vitro.
B M, Glaser, P A, Campochiaro, J L, Davis, J A, Jerdan   +3 more
openaire   +2 more sources