Reverse genetics rescue of sylvatic dengue viruses [PDF]
Journal of VirologyThere are an unknown number of sylvatic (“sylvan” = “of the forest”) dengue viruses currently being sustained by nonhuman primates and mosquitoes in the forests of Africa and Asia.
Paul Gendler +6 more
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Engineering Nipah virus: Reverse genetics as a gateway to novel drug discovery [PDF]
New Microbes and New InfectionsNipah virus (NiV) is a highly pathogenic and re-emerging virus that requires containment in biosafety level 4 (BSL-4) laboratories. The limited accessibility of these high-security facilities poses major obstacles to investigating immunopathogenesis and ...
Muralidharan Menon Arjun +1 more
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Highs and Lows in Calicivirus Reverse Genetics [PDF]
VirusesIn virology, the term reverse genetics refers to a set of methodologies in which changes are introduced into the viral genome and their effects on the generation of infectious viral progeny and their phenotypic features are assessed.
Ángel L. Álvarez +7 more
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Exploring enterovirus pathogenesis and cancer therapy potential through reverse genetics [PDF]
Biosafety and HealthOver the past decades, oncolytic viruses have emerged as prominent therapeutic agents with significant potential for anticancer treatment. Enteroviruses (EVs) have garnered particular attention due to their specific tropism for various types of tumor ...
Shijin Wang +6 more
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Marburg Virus Reverse Genetics Systems [PDF]
Viruses, 2016 The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses.
Kristina Maria Schmidt, Elke Mühlberger
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Reverse genetics in ecological research.
PLoS ONE, 2008 By precisely manipulating the expression of individual genetic elements thought to be important for ecological performance, reverse genetics has the potential to revolutionize plant ecology.
Jens Schwachtje +2 more
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Research progress and applications of reverse genetics systems for infectious bronchitis virus [PDF]
Poultry ScienceInfectious bronchitis virus (IBV) poses a persistent threat to global poultry health, driving the need for advanced molecular tools to study and combat this pathogen.
Yinghao Zhao
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A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease. [PDF]
PLoS ONEThe encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen +2 more
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Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons [PDF]
Bio-ProtocolHuman astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali +2 more
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Developments in Negative-Strand RNA Virus Reverse Genetics [PDF]
MicroorganismsMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang +6 more
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