Results 1 to 10 of about 666,471 (318)

Highs and Lows in Calicivirus Reverse Genetics [PDF]

Viruses
In virology, the term reverse genetics refers to a set of methodologies in which changes are introduced into the viral genome and their effects on the generation of infectious viral progeny and their phenotypic features are assessed.
Ángel L. Álvarez, Aroa Arboleya, Fábio A. Abade dos Santos, Alberto García-Manso, Inés Nicieza, Kevin P. Dalton, Francisco Parra, José M. Martín-Alonso   +7 more
doaj   +4 more sources

Reverse genetics in virology: A double edged sword

Biosafety and Health, 2022
Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.
Hongyu Chen, Hongqi Liu, Xiaozhong Peng
doaj   +2 more sources

Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System

mBio, 2022
Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans.
Avan Antia, Amanda N. Pinski, Siyuan Ding   +2 more
doaj   +2 more sources

Reverse genetics rescue of sylvatic dengue viruses [PDF]

Journal of Virology
There are an unknown number of sylvatic (“sylvan” = “of the forest”) dengue viruses currently being sustained by nonhuman primates and mosquitoes in the forests of Africa and Asia.
Paul Gendler, Arturo Barbachano-Guerrero, Samuel D. Chappell, Oshani C. Ratnayake, Samantha M. Pinto, Rushika Perera, Sara L. Sawyer   +6 more
doaj   +2 more sources

Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host [PDF]

, 2013
Schmallenberg virus (SBV) is an emerging orthobunyavirus of ruminants associated with outbreaks of congenital malformations in aborted and stillborn animals.
A Bridgen, A Bridgen, A Kohl, AC Lowen, AJ Della-Porta, Alain Kohl, Andrew Shaw, Anna Janowicz, AR Elbers, B Hoffmann, B Xu, CA Evans, Catherine Lamm, Claudio Murgia, CM Southam, CT Walter, D Thomas, DH Cybinski, Esther Schnettler, Eva McGregor, F Arnaud, Friedemann Weber, G Barry, G Blakqori, G Blakqori, G Neumann, Gerald Barry, Ilaria M. Piras, JI Gillespie, JL Farmer, K Kupferschmidt, K Peperkamp, K Terasaki, K Wernike, KE Jones, Kerstin Hahn, KV Goller, LD Rasmussen, M Beer, M Bouloy, M Caporale, M Dominguez, M Palmarini, M Ratinier, M Spiegel, M Varela, Mandy Glass, Marco Caporale, Mariana Varela, Martin Beer, Massimo Palmarini, Melanie McFarlane, MJ Killip, MM Garigliany, MM Garigliany, NR Saunders, OR Causey, P Vialat, PJ Hotez, PT Leisnham, R Muller, RS Azevedo, S Shimshony, SE Randolph, T Ikegami, T Yanase, UJ Buchholz, V Herder, Vanessa Herder, VH Leonard, Wolfgang Baumgärtner, X Shi, Y Ogawa, YK Liao   +73 more
core   +10 more sources

A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease. [PDF]

PLoS ONE
The encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen, Matthew J Abbott, Alyssa B Evans   +2 more
doaj   +2 more sources

Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons [PDF]

Bio-Protocol
Human astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali, David Noyvert, Valeria Lulla   +2 more
doaj   +2 more sources

Developments in Negative-Strand RNA Virus Reverse Genetics [PDF]

Microorganisms
Many epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang, Jinyan Wu, Xiaoan Cao, Long Xu, Junhuang Wu, Haiyan Ding, Youjun Shang   +6 more
doaj   +2 more sources

Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation [PDF]

Virology Journal
Influenza is a highly contagious acute viral illness that affects the respiratory system, posing a significant global public health concern. Influenza B virus (IBV) causes annual seasonal epidemics.
Ghayyas Ud Din, Chunchen Wu, Zahra Tariq, Kinza Hasham, Muhammad Nabeel Amjad, Bei Shen, Lihuan Yue, Muhammad Asif Raza, Muhammad Awais Ashraf, Lingdie Chen, Yihong Hu   +10 more
doaj   +2 more sources

Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine [PDF]

Journal of Virology
Control of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection ...
Tendai A. M. Mlingo, Jacques Theron, Nobalanda B. Mokoena   +2 more
doaj   +2 more sources