Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation [PDF]
Virology JournalInfluenza is a highly contagious acute viral illness that affects the respiratory system, posing a significant global public health concern. Influenza B virus (IBV) causes annual seasonal epidemics.
Ghayyas Ud Din +10 more
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Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine [PDF]
Journal of VirologyControl of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection ...
Tendai A. M. Mlingo +2 more
doaj +2 more sources
Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. [PDF]
PLoS ONEAvian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables ...
Katharina Bilotti +13 more
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Reverse genetics in virology: A double edged sword
Biosafety and Health, 2022 Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application.
Hongyu Chen, Hongqi Liu, Xiaozhong Peng
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Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis
Viruses, 2021 Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto +3 more
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Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta [PDF]
, 2017 Preventive chemotherapy has long been practiced against nematode parasites of livestock, leading to widespread drug resistance, and is increasingly being adopted for eradication of human parasitic nematodes even though it is similarly likely to lead to ...
Bisset, Stewart A +6 more
core +11 more sources
Reverse Genetics System for Rabbit vesivirus
Frontiers in Microbiology, 2020 Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
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Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
Инфекция и иммунитет, 2023 Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of ...
N. V. Larionova +4 more
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Plant Cellular and Molecular Biotechnology: Following Mariotti's Steps [PDF]
, 2019 This review is dedicated to the memory of Prof. Domenico Mariotti, who significantly contributed to establishing the Italian research community in Agricultural Genetics and carried out the first experiments of Agrobacterium-mediated plant genetic ...
Caretto, Sofia +11 more
core +1 more source