Results 11 to 20 of about 666,471 (318)

Reverse genetics in ecological research.

PLoS ONE, 2008
By precisely manipulating the expression of individual genetic elements thought to be important for ecological performance, reverse genetics has the potential to revolutionize plant ecology.
Jens Schwachtje, Susan Kutschbach, Ian T Baldwin   +2 more
doaj   +5 more sources

Application of versatile reverse genetics system for feline coronavirus [PDF]

Microbiology Spectrum
Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida, Tomokazu Tamura, Yudai Kuroda, Takasuke Fukuhara, Ken Maeda, Keita Matsuno   +5 more
doaj   +2 more sources

One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. [PDF]

PLoS ONE
Avian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables ...
Katharina Bilotti, Sarah Keep, Andrew P Sikkema, John M Pryor, James Kirk, Katalin Foldes, Nicole Doyle, Ge Wu, Graham Freimanis, Giulia Dowgier, Oluwapelumi Adeyemi, S Kasra Tabatabaei, Gregory J S Lohman, Erica Bickerton   +13 more
doaj   +2 more sources

Development of a reverse genetics system for West Nile virus (Kunjin type). [PDF]

Front Vet Sci
Wu Z, Hu T, Zhou Z, He Y, Wang T, Wang M, Jia R, Zhu D, Liu M, Zhao X, Yang Q, Wu Y, Zhang S, Huang J, Ou X, Sun D, Tian B, Cheng A, Chen S.   +18 more
europepmc   +3 more sources

Generation of Recombinant Authentic Live Attenuated Human Rotavirus Vaccine Strain RIX4414 (Rotarix^®) from Cloned cDNAs Using Reverse Genetics [PDF]

Viruses
The live attenuated human rotavirus vaccine strain RIX4414 (Rotarix®) is used worldwide to prevent severe rotavirus-induced diarrhea in infants. This strain was attenuated through the cell culture passaging of its predecessor, human strain 89-12, which ...
Saori Fukuda, Masanori Kugita, Kanako Kumamoto, Yuki Akari, Yuki Higashimoto, Shizuko Nagao, Takayuki Murata, Tetsushi Yoshikawa, Koki Taniguchi, Satoshi Komoto   +9 more
doaj   +2 more sources

A Novel Mutation in the Upstream Open Reading Frame of the CDKN1B Gene Causes a MEN4 Phenotype [PDF]

, 2013
PubMed ID: 23555276This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
A Borriello, A Falchetti, A Mehta, A Ozawa, A Wiestner, A Yoon, Alessandro Quattrone, BG Luukkonen, C Le Sage, Carla Scaroni, CC Spevak, CJ Sherr, CJ Sherr, D Malanga, Daniela Regazzo, Denis Ciato, DR Morris, Elena Pardi, Filomena Cetani, Francesca Boaretto, Franco Mantero, G Beffagna, G Johannes, G Occhi, GC Scheper, Giampaolo Trivellin, Gianluca Occhi, Giuseppe Opocher, IM Chu, J Costa-Guda, J Slingerland, K Wethmar, L Liu, L Quintanilla-Martinez, M Georgitsi, M Kozak, M Kozak, M Kullmann, M Matsui, Marshall S. Horwitz, MD Larrea, ML Crowe, ML Fero, Márta Korbonits, Natalia S. Pellegata, NS Pellegata, P Chomczynski, RV Thakker, S Igreja, S Molatore, S Ye, Sara Bobisse, SE Calvo, Sergio Ferasin, SI Reed, SK Agarwal, U Göpfert, Viktoryia Sidarovich, W Zhou, WK Miskimins, Y Wen   +60 more
core   +27 more sources

Generation of rescued Japanese encephalitis virus genotype 1 from infectious full-size clone using reverse genetics [PDF]

Heliyon
Japanese encephalitis virus (JEV) is a pathogen responsible for high mortality and morbidity rates among children with encephalitis. Since JEV genotype 1 (GI) is the most prevalent strain in South Korea these days, corresponding research and vaccine ...
Sehyun Kim, Min Sun Kim, Aleksandra Nowakowska, Heejae Choi, Hee Won Bang, Young Bong Kim, Hee-Jung Lee   +6 more
doaj   +2 more sources

Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis

Viruses, 2021
Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto, Saori Fukuda, Takayuki Murata, Koki Taniguchi   +3 more
doaj   +1 more source

Reverse genetics in eukaryotes [PDF]

Biology of the Cell, 2010
Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations.
Hardy, Serge, Legagneux, Vincent, Audic, Yann, Paillard, Luc   +3 more
openaire   +3 more sources

Reverse Genetics System for Rabbit vesivirus

Frontiers in Microbiology, 2020
Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez, Alberto García-Manso, Kevin P. Dalton, José M. Martín-Alonso, Inés Nicieza, Ana Podadera, Maikel Acosta-Zaldívar, Daniel de Llano, Francisco Parra   +8 more
doaj   +1 more source