Optimized properties of live vaccine influenza reassortant strains obtained by reverse genetics
Инфекция и иммунитет, 2023 Classical reassortment in developing chicken eggs is a well-established technique for obtaining LAIV strains. Naturally generated reassortant vaccine strains are characterized by high reproductive capacity, genetically stable characteristics of ...
N. V. Larionova +4 more
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Plant Cellular and Molecular Biotechnology: Following Mariotti's Steps [PDF]
, 2019 This review is dedicated to the memory of Prof. Domenico Mariotti, who significantly contributed to establishing the Italian research community in Agricultural Genetics and carried out the first experiments of Agrobacterium-mediated plant genetic ...
Caretto, Sofia +11 more
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Development of a novel equine influenza virus live-attenuated vaccine [PDF]
, 2018 H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a
Chambers, Thomas M. +5 more
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Recent advances in malaria genomics and epigenomics [PDF]
, 2016 Malaria continues to impose a significant disease burden on low- and middle-income countries in the tropics. However, revolutionary progress over the last 3 years in nucleic acid sequencing, reverse genetics, and post-genome analyses has generated step ...
Kirchner, Sebastian +2 more
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Marburg Virus Reverse Genetics Systems
Viruses, 2016 The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses.
Kristina Maria Schmidt, Elke Mühlberger
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Flavivirus reverse genetic systems, construction techniques and applications: A historical perspective [PDF]
, 2015 The study of flaviviruses, which cause some of the most important emerging tropical and sub-tropical human arbovirus diseases, has greatly benefited from the use of reverse genetic systems since its first development for yellow fever virus in 1989 ...
Aubry, Fabien +3 more
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Recent advances in Reovirales viruses reverse genetics research
Virus Research, 2022 Reovirales are segmented double-strand RNA viruses with a broad host range that pose a serious threat to human and animal health. However, there are numerous viral species within the Reovirales, some of which have lagged behind other RNA viruses in the ...
Xinyu Zhang, Feng Wen
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Engineering recombinant orsay virus directly in the metazoan host Caenorhabditis elegans [PDF]
, 2014 The recent identification of Orsay virus, the first virus that is capable of naturally infecting Caenorhabditis elegans, provides a unique opportunity to explore host-virus interaction studies in this invaluable model organism.
Franz, Carl J +2 more
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Efficient generation of recombinant influenza A viruses employing a new approach to overcome the genetic instability of HA segments. [PDF]
PLoS ONE, 2015 Influenza A viruses (IAVs) are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry.
Ahmed Mostafa +5 more
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Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR. [PDF]
, 2018 Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here,
Akbari, Omar S +2 more
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