Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System
mBio, 2022 Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans.
Avan Antia +2 more
doaj +1 more source
Development of a novel equine influenza virus live-attenuated vaccine [PDF]
, 2018 H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a
Chambers, Thomas M. +5 more
core +1 more source
Reverse genetics in eukaryotes [PDF]
Biology of the Cell, 2010 Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations.
Hardy, Serge +3 more
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Recent advances in malaria genomics and epigenomics [PDF]
, 2016 Malaria continues to impose a significant disease burden on low- and middle-income countries in the tropics. However, revolutionary progress over the last 3 years in nucleic acid sequencing, reverse genetics, and post-genome analyses has generated step ...
Kirchner, Sebastian +2 more
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Flavivirus reverse genetic systems, construction techniques and applications: A historical perspective [PDF]
, 2015 The study of flaviviruses, which cause some of the most important emerging tropical and sub-tropical human arbovirus diseases, has greatly benefited from the use of reverse genetic systems since its first development for yellow fever virus in 1989 ...
Aubry, Fabien +3 more
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Recent advances in Reovirales viruses reverse genetics research
Virus Research, 2022 Reovirales are segmented double-strand RNA viruses with a broad host range that pose a serious threat to human and animal health. However, there are numerous viral species within the Reovirales, some of which have lagged behind other RNA viruses in the ...
Xinyu Zhang, Feng Wen
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Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR. [PDF]
, 2018 Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here,
Akbari, Omar S +2 more
core +2 more sources
Efficient generation of recombinant influenza A viruses employing a new approach to overcome the genetic instability of HA segments. [PDF]
PLoS ONE, 2015 Influenza A viruses (IAVs) are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry.
Ahmed Mostafa +5 more
doaj +1 more source
Engineering recombinant orsay virus directly in the metazoan host Caenorhabditis elegans [PDF]
, 2014 The recent identification of Orsay virus, the first virus that is capable of naturally infecting Caenorhabditis elegans, provides a unique opportunity to explore host-virus interaction studies in this invaluable model organism.
Franz, Carl J +2 more
core +2 more sources
Schmallenberg virus pathogenesis, tropism and interaction with the innate immune system of the host [PDF]
, 2013 Schmallenberg virus (SBV) is an emerging orthobunyavirus of ruminants associated with outbreaks of congenital malformations in aborted and stillborn animals.
A Bridgen +73 more
core +3 more sources