Exploring enterovirus pathogenesis and cancer therapy potential through reverse genetics [PDF]
Biosafety and HealthOver the past decades, oncolytic viruses have emerged as prominent therapeutic agents with significant potential for anticancer treatment. Enteroviruses (EVs) have garnered particular attention due to their specific tropism for various types of tumor ...
Shijin Wang +6 more
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A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease. [PDF]
PLoS ONEThe encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen +2 more
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Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons [PDF]
Bio-ProtocolHuman astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on
Hashim Ali +2 more
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Developments in Negative-Strand RNA Virus Reverse Genetics [PDF]
MicroorganismsMany epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and ...
Mengyi Wang +6 more
doaj +2 more sources
Application of Reverse Genetics in Functional Genomics of Potyvirus [PDF]
Viruses, 2020 Numerous potyvirus studies, including virus biology, transmission, viral protein function, as well as virus–host interaction, have greatly benefited from the utilization of reverse genetic techniques.
Maathavi Kannan +4 more
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Generation of Recombinant Authentic Live Attenuated Human Rotavirus Vaccine Strain RIX4414 (Rotarix®) from Cloned cDNAs Using Reverse Genetics [PDF]
VirusesThe live attenuated human rotavirus vaccine strain RIX4414 (Rotarix®) is used worldwide to prevent severe rotavirus-induced diarrhea in infants. This strain was attenuated through the cell culture passaging of its predecessor, human strain 89-12, which ...
Saori Fukuda +9 more
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Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication [PDF]
Viruses, 2022 The COVID-19 pandemic continues to threaten healthcare systems worldwide due to the limited access to vaccines, suboptimal treatment options, and the continuous emergence of new and more transmissible SARS-CoV-2 variants.
Ulrik Fahnøe +11 more
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Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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Influenza Reverse Genetics—Historical Perspective [PDF]
Cold Spring Harbor Perspectives in Medicine, 2020 The generation of wild-type, mutant, and reassortant influenza viruses from viral cDNAs (reverse genetics) is now a basic molecular virology technique in many influenza virus laboratories. Here, I describe the original RNA polymerase I reverse genetics system and the modifications that have been developed in past years.
G. Neumann
openaire +3 more sources
Reverse genetics for mammalian reovirus [PDF]
Methods, 2011 Mammalian orthoreoviruses (reoviruses) are highly tractable models for studies of viral replication and pathogenesis. The versatility of reovirus as an experimental model has been enhanced by development of a plasmid-based reverse genetics system. Infectious reovirus can be recovered from cells transfected with plasmids encoding cDNAs of each reovirus ...
Karl W. Boehme +3 more
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