Results 11 to 20 of about 3,000 (187)

GeneXpert MTB/RIF Ultra performance to detect uncommon rpoB mutations in Mycobacterium tuberculosis

BMC Research Notes, 2023
Objective To investigate the performance of GeneXpert MTB/RIF Ultra to accurately detect rifampicin resistance for less common rpoB mutations that potentially confer phenotypic resistance, we tested 28 such Mycobacterium tuberculosis cultures with Xpert ...
Leen Rigouts, Jelle Keysers, Reenaers Rabab, Kristina Fissette, Armand van Deun, Bouke Catherine de Jong   +5 more
doaj   +1 more source

Prevalence and drivers of false-positive rifampicin-resistant Xpert MTB/RIF results: a prospective observational study in Rwanda

The Lancet Microbe, 2020
Summary: Background: The Xpert MTB/RIF (Xpert) assay is used globally to rapidly diagnose tuberculosis and resistance to rifampicin. We investigated the frequency and predictors of false-positive findings of rifampicin resistance with Xpert. Methods: We
Jean Claude Semuto Ngabonziza, MSc, Tom Decroo, PhD, Patrick Migambi, MSc, Yves Mucyo Habimana, MD, Armand Van Deun, PhD, Conor J Meehan, PhD, Gabriela Torrea, PhD, Faridath Massou, PharmD, Willem Bram de Rijk, BS, Bertin Ushizimpumu, BS, Esdras Belamo Niyigena, BS, Emil Ivan, PhD, Jules Mugabo Semahore, MD, Jean Baptiste Mazarati, PhD, Corinne Simone Merle, PhD, Philip Supply, PhD, Dissou Affolabi, ProfPhD, Leen Rigouts, PhD, Bouke Catherine de Jong, ProfPhD   +18 more
doaj   +1 more source

Mutations outside the rifampicin resistance-determining region associated with rifampicin resistance in Mycobacterium tuberculosis [PDF]

Journal of Antimicrobial Chemotherapy, 2011
Ninety-six percent of rifampicin resistance in Mycobacterium tuberculosis was shown to be associated with mutations inside the 81 bp rifampicin resistance-determining region (RRDR) located in the centre of the rpoB gene. The detection of rifampicin resistance by targeting the RRDR failed to match with a resistant phenotype in 4% of all cases. Our study
Lau, TCK, Siu, GKH, Zhang, Y, Lau, RWT, Ho, PL, Yew, WW, Tsui, SKW, Cheng, VCC, Yuen, KY, Yam, WC   +9 more
openaire   +4 more sources

Cell membrane components of Brucella melitensis play important roles in the resistance of low-level rifampicin.

PLoS Neglected Tropical Diseases, 2020
Brucella spp. are facultative intracellular pathogens that can persistently colonize host cells and cause the zoonosis- brucellosis. The WHO recommended a treatment for brucellosis that involves a combination of doxycycline, rifampicin, or streptomycin ...
Xiaowen Yang, Tonglei Wu, Wenxiao Liu, Guozhong Tian, Hongyan Zhao, Dongri Piao, Hai Jiang, Qingmin Wu   +7 more
doaj   +1 more source

Selective Pressure by Rifampicin Modulates Mutation Rates and Evolutionary Trajectories of Mycobacterial Genomes

Microbiology Spectrum, 2023
Resistance to the frontline antibiotic rifampicin constitutes a challenge to the treatment and control of tuberculosis. Here, we analyzed the mutational landscape of Mycobacterium smegmatis during long-term evolution with increasing concentrations of ...
E. Cebrián-Sastre, A. Chiner-Oms, R. Torres-Pérez, I. Comas, J. C. Oliveros, J. Blázquez, A. Castañeda-García   +6 more
doaj   +1 more source

Resistance to rifampicin: a review [PDF]

The Journal of Antibiotics, 2014
Resistance to rifampicin (RIF) is a broad subject covering not just the mechanism of clinical resistance, nearly always due to a genetic change in the β subunit of bacterial RNA polymerase (RNAP), but also how studies of resistant polymerases have helped us understand the structure of the enzyme, the intricacies of the transcription process and its ...
openaire   +2 more sources

Increased rate of enteric bacteria as cause of periprosthetic joint infections in patients with liver cirrhosis

BMC Infectious Diseases, 2022
Introduction Periprosthetic joint infections (PJI) are a major complication in joint-arthroplasty. Rifampicin is often used as an additional agent to treat PJI, because it penetrates bacterial biofilms.
Uta S. Koepf, Sebastian Scheidt, Gunnar T. R. Hischebeth, Christian P. Strassburg, Dieter C. Wirtz, Thomas M. Randau, Philipp Lutz   +6 more
doaj   +1 more source

Airway microecology in rifampicin-resistant and rifampicin-sensitive pulmonary tuberculosis patients

BMC Microbiology, 2022
Abstract Background Pulmonary tuberculosis is a chronic infectious disease of the respiratory system. It is still one of the leading causes of death from a single infectious disease, but it has been stuck in the study of a single pathogen. Recent studies have shown that many diseases are associated with disruption of the
Xingshan Cai, Yang Luo, Yuanliang Zhang, Yuan Lin, Bitong Wu, Zhizhong Cao, Zuqiong Hu, Xingyi Wu, Shouyong Tan   +8 more
openaire   +3 more sources

Detecting rifampin and isoniazid resistance in Mycobacterium tuberculosis direct from patient sputum using an automated integrated system

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, 2022
While there has been progress in detection of drug resistant tuberculosis globally, WHO estimates only about half of the patients with bacteriologically confirmed tuberculosis were tested for rifampicin resistance over the past two years.
Rebecca E. Colman, Christopher Hagan, Peter Chiles, Marva Seifert, Donald G. Catanzaro, Alexander V. Kukhtin, Ryan Norville, Laura Hauns, Arial Bueno, Rebecca C. Holmberg, Christopher G. Cooney, Timothy C. Rodwell   +11 more
doaj   +1 more source

Could high-concentration rifampicin kill rifampicin-resistant M. tuberculosis? Rifampicin MIC test in rifampicin-resistant isolates from patients with osteoarticular tuberculosis [PDF]

Journal of Orthopaedic Surgery and Research, 2014
Several studies have shown that the intralesional concentration of rifampicin in osteoarticular tuberculosis is typically at a subtherapeutic level. Sustained or controlled release by novel drug delivery systems has been investigated to maintain an effective rifampicin concentration, but the local administration of rifampicin remains controversial ...
Zhang, Zehua, Dai, Fei, Luo, Fei, Zhong, Min, Huang, Zhenggu, Hou, Tianyong, Xu, Jianzhong   +6 more
openaire   +2 more sources