Results 1 to 10 of about 240,794 (319)
tRNATrp as primer for RNA-directed DNA polymerase: structural determinants of function. [PDF]
Journal of Biological Chemistry, 1979 The specific interactions between the RNA-directed DNA polymerase of avian oncornavirus and the tRNATrp primer required for initiation of viral DNA synthesis in vitro were examined. Two distinct interactions, stable binding of the tRNATrp to the enzyme and initiation of viral DNA synthesis by the enzyme with tRNATrp as primer, were characterized as to ...
J M Bishop +3 more
semanticscholar +4 more sources
Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles [PDF]
Journal of Virology, 1977 An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity.
Kohno, M, Tanaka, H
semanticscholar +5 more sources
RNA-Directed DNA Polymerase and Virus-Induced Leukemia in Mice [PDF]
Proceedings of the National Academy of Sciences, 1973 Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme.
Alan M. Wu +2 more
semanticscholar +6 more sources
Polymerase IV occupancy at RNA-directed DNA methylation sites requires SHH1 [PDF]
Nature, 2013 DNA methylation is an epigenetic modification that plays critical roles in gene silencing, development, and genome integrity. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and targeted by 24 nt small interfering RNAs (siRNAs) through a pathway termed RNA-directed DNA methylation (RdDM)1.
Dinshaw J. Patel +8 more
semanticscholar +6 more sources
Human placentas contain a specific inhibitor of RNA-directed DNA polymerase. [PDF]
Proceedings of the National Academy of Sciences, 1981 Human placental extracts contain a specific inhibitor of mammalian retroviral RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) activity. This inhibitor copurifies with retrovirus-like particles in human placental extracts.
Jay A. Nelson +2 more
semanticscholar +5 more sources
Endogenous RNA-Directed DNA Polymerase Activity in Uninfected Chicken Embryos [PDF]
Proceedings of the National Academy of Sciences, 1972 Early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous RNA-directed DNA polymerase activity. This endogenous DNA polymerase activity was not increased after mixture of soluble DNA polymerases isolated from chicken embryos with disrupted chicken embryo cells.
Chil-Yong Kang, Howard M. Temin
openaire +4 more sources
Inhibition of RNA-directed DNA polymerase by aurintricarboxylic acid.
Nucleic Acids Research, 1976 Commercial-grade aurintricarboxylic acid (ATA) inhibits poly(A), poly(C) and viral RNA-directed DNA synthesis by detergent-disrupted virions of Moloney murine leukemia virus. Paper chromatography of crude ATA yields two active components, which appear to behave identically, and at least two inactive components.
Kenneth F. Manly, Jean F. Givens
openaire +4 more sources
DNA polymerase of reticuloendotheliosis virus: Inability to detect endogenous RNA-directed DNA synthesis [PDF]
Virology, 1975 Abstract Purified preparations of reticuloendotheliosis virus (REV) appear to lack the endogenous RNase-sensitive DNA polymerase activity present in most, if not all, avian RNA tumor viruses. Although no endogenous RNA-directed DNA polymerase could be detected in REV, we have been able to demonstrate the presence of a virion-associated DNA polymerase
Kieras, Rita M., Faras, Anthony J.
openaire +5 more sources
Human Leukemic Cells: RNA‐Directed DNA Polymerase [PDF]
European Journal of Biochemistry, 1974 The enzyme(s) RNA‐dependent DNA polymerase (reverse transcriptase) has been isolated from human lymphocytic cell lines which have been derived from patients with leukemia, infectious mononucleosis, and from normal healthy donors. The reverse transcriptase was purified through high‐speed glycerol gradients, and by DEAE‐cellulose and phosphocellulose ...
George E. Foley +3 more
openaire +5 more sources
Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase [PDF]
Journal of Virology, 1977 Tryptophanyl-tRNA was specifically labeled at the 3' end with [3H]tryptophan and cleaved in half with RNase under denaturing conditions, and the 3' half was shown to hybridize exclusively at the 5' end of avian myeloblastosis virus RNA. The RNA-dependent DNA polymerase of avian myeloblastosis virus is capable of efficiently binding the 3' half of the ...
R D Brown, R W Armentrout
openaire +4 more sources