Results 1 to 10 of about 325,869 (333)

Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase [PDF]

Journal of Virology, 1977
Tryptophanyl-tRNA was specifically labeled at the 3' end with [3H]tryptophan and cleaved in half with RNase under denaturing conditions, and the 3' half was shown to hybridize exclusively at the 5' end of avian myeloblastosis virus RNA. The RNA-dependent DNA polymerase of avian myeloblastosis virus is capable of efficiently binding the 3' half of the ...
R. Don Brown, Richard W. Armentrout
openalex   +3 more sources

DNA polymerase of reticuloendotheliosis virus: Inability to detect endogenous RNA-directed DNA synthesis [PDF]

Virology, 1975
Abstract Purified preparations of reticuloendotheliosis virus (REV) appear to lack the endogenous RNase-sensitive DNA polymerase activity present in most, if not all, avian RNA tumor viruses. Although no endogenous RNA-directed DNA polymerase could be detected in REV, we have been able to demonstrate the presence of a virion-associated DNA polymerase
Rita M. Kieras, Anthony J. Faras
openalex   +5 more sources

Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles [PDF]

Journal of Virology, 1977
An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity.
Michiaki Kohno, Hajime Tanaka
openalex   +3 more sources

Human Leukemic Cells: RNA‐Directed DNA Polymerase [PDF]

European Journal of Biochemistry, 1974
The enzyme(s) RNA‐dependent DNA polymerase (reverse transcriptase) has been isolated from human lymphocytic cell lines which have been derived from patients with leukemia, infectious mononucleosis, and from normal healthy donors. The reverse transcriptase was purified through high‐speed glycerol gradients, and by DEAE‐cellulose and phosphocellulose ...
L.S. Desai, Diane L. SHORT, Orrie M. Friedman, George E. Foley   +3 more
openalex   +4 more sources

Genetic and structural study of DNA-directed RNA polymerase II of Trypanosoma brucei, towards the designing of novel antiparasitic agents [PDF]

PeerJ, 2017
Trypanosoma brucei brucei (TBB) belongs to the unicellular parasitic protozoa organisms, specifically to the Trypanosoma genus of the Trypanosomatidae class.
Louis Papageorgiou, Vasileios Megalooikonomou, Dimitrios Vlachakis   +2 more
doaj   +4 more sources

Human placentas contain a specific inhibitor of RNA-directed DNA polymerase. [PDF]

Proceedings of the National Academy of Sciences, 1981
Human placental extracts contain a specific inhibitor of mammalian retroviral RNA-directed DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) activity. This inhibitor copurifies with retrovirus-like particles in human placental extracts.
Jay A. Nelson, Jay A. Levy, Jo‐Ann C. Leong   +2 more
openalex   +4 more sources

tRNATrp as primer for RNA-directed DNA polymerase: structural determinants of function.

Journal of Biological Chemistry, 1979
The specific interactions between the RNA-directed DNA polymerase of avian oncornavirus and the tRNATrp primer required for initiation of viral DNA synthesis in vitro were examined. Two distinct interactions, stable binding of the tRNATrp to the enzyme and initiation of viral DNA synthesis by the enzyme with tRNATrp as primer, were characterized as to ...
Barbara Cordell, Ronald Swanstrom, H. M. Goodman, J. Michael Bishop   +3 more
openalex   +3 more sources

Cluster analysis of selected Mycobacterium species using rifampicin resistance DNA-directed RNA polymerase beta subunit (rpoB) gene

International Journal of Infectious Diseases, 2020
O.S. Amoo, I. Taiwo, F. Anwoju
doaj   +2 more sources

Inhibiting the oligomerization of mycobacterial DNA-directed RNA polymerase (RNAP) using natural compound via in-silico techniques

Medicine in Novel Technology and Devices
Mycobacterium tuberculosis (Mtb) is responsible for the spread of tuberculosis (TB). The current study employed virtual screening of 2569 natural compounds against the DNA-directed RNA polymerase (RNAP) of Mtb to identify the possible binders that can ...
Ehssan H. Moglad
doaj   +2 more sources

RNA-mediated in vivo Directed Evolution in Yeast

Bio-Protocol, 2022
Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
doaj   +1 more source