Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications [PDF]
ResearchT7 RNA polymerase (T7 RNAP) is the most widely used enzyme for synthesizing therapeutic mRNA. However, RNA transcribed by T7 RNAP often contains double-stranded RNA (dsRNA) by-products that trigger innate immune responses and complicate purification ...
Weitong Qin +6 more
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RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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eLife, 2022 In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer +5 more
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SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
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Animals, 2023 The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes.
Ghulam Mustafa +6 more
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Metal A and metal B sites of nuclear RNA polymerases Pol IV and Pol V are required for siRNA-dependent DNA methylation and gene silencing. [PDF]
PLoS ONE, 2009 Plants are unique among eukaryotes in having five multi-subunit nuclear RNA polymerases: the ubiquitous RNA polymerases I, II and III plus two plant-specific activities, nuclear RNA polymerases IV and V (previously known as Polymerases IVa and IVb).
Jeremy R Haag +2 more
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Human Leukemic Cells: RNA‐Directed DNA Polymerase [PDF]
European Journal of Biochemistry, 1974 The enzyme(s) RNA‐dependent DNA polymerase (reverse transcriptase) has been isolated from human lymphocytic cell lines which have been derived from patients with leukemia, infectious mononucleosis, and from normal healthy donors. The reverse transcriptase was purified through high‐speed glycerol gradients, and by DEAE‐cellulose and phosphocellulose ...
L S, Desai +3 more
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Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. [PDF]
PLoS Genetics, 2008 DNA methylation directed by 24-nucleotide small RNAs involves the small RNA-binding protein ARGONAUTE4 (AGO4), and it was previously shown that AGO4 localizes to nucleolus-adjacent Cajal bodies, sites of snRNP complex maturation. Here we demonstrate that
Carey F Li +5 more
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The Rise and Fall of Billionaire siRNAs during Reproductive Development in Rice
Plants, 2022 RNA polymerase IV-dependent siRNAs, usually 24 nt in length, function in the RNA-directed DNA methylation that is responsible for de novo methylation in plants.
Lili Wang +3 more
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RNA-Directed DNA Polymerase and Virus-Induced Leukemia in Mice [PDF]
Proceedings of the National Academy of Sciences, 1973 Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme.
A M, Wu, R C, Ting, R C, Gallo
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