Results 11 to 20 of about 654 (199)
The TDG protein environment connects active DNA demethylation with chromatin and RNA biology
Cellular and Molecular Life SciencesUnderstanding the interplay between DNA methylation and gene expression remains a challenge. This study explores the proteome of active DNA demethylation in murine embryonic stem cells (mESC), focusing on the base-excision-repair (BER) step initiated by ...
Federica Richina +9 more
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Tendon Organoids Enable Functional Tendon Rejuvenation Through ALKBH5‐Dependent RNA Demethylation
Advanced ScienceAdult tendon injuries pose a major clinical challenge due to limited self‐repair capacity, resulting in suboptimal regeneration. Although tendon stem/progenitor cells (TSPCs) are pivotal for tendon engineering, achieving microstructure and functional ...
Tian Qin +14 more
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DNA Demethylation In Vitro: Involvement of RNA [PDF]
Cell, 1996 An in vitro system for studying DNA demethylation has been established using extracts from tissue culture cells. This reaction, which is unusually resistant to proteinase K, takes place through the removal of a 5-methylcytosine nucleotide unit from the DNA substrate and its conversion to an RNase-sensitive form. It is likely that this represents the in
Weiss, Alona +3 more
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RNA-directed DNA methylation and demethylation in plants [PDF]
Science in China Series C: Life Sciences, 2009 RNA-directed DNA methylation (RdDM) is a nuclear process in which small interfering RNAs (siRNAs) direct the cytosine methylation of DNA sequences that are complementary to the siRNAs. In plants, double stranded-RNAs (dsRNAs) generated by RNA-dependent RNA polymerase 2 (RDR2) serve as precursors for Dicer-like 3 dependent biogenesis of 24-nt siRNAs ...
Viswanathan, Chinnusamy, Jian-Kang, Zhu
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Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA
Chemical Science, 2023 Methylation in the 5′ handle of gRNA disrupts the interaction between the Cas12a and gRNA. The deactivated CRISPR can be reactivated by demethylases, that enabled the development of biosensors and the precise regulation of gene expression.
Zhian Hu +8 more
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Cell Reports, 2023 Summary: Perturbing the transcriptome of mammalian oocytes results in meiotic failure. We previously reported that RNA-exosome-associated RNase, EXOSC10, degrades unwanted protein-coding RNA and processes ribosomal RNA to ensure proper oocyte maturation.
Di Wu, Jurrien Dean
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Viral AlkB proteins repair RNA damage by oxidative demethylation [PDF]
Nucleic Acids Research, 2008 Bacterial and mammalian AlkB proteins are iron(II)- and 2-oxoglutarate-dependent dioxygenases that reverse methylation damage, such as 1-methyladenine and 3-methylcytosine, in RNA and DNA. An AlkB-domain is encoded by the genome of numerous single-stranded, plant-infecting RNA viruses, the majority of which belong to the Flexiviridae family.
van den Born, Erwin +6 more
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Gadd45a is an RNA binding protein and is localized in nuclear speckles.
PLoS ONE, 2011 BackgroundThe Gadd45 proteins play important roles in growth control, maintenance of genomic stability, DNA repair, and apoptosis. Recently, Gadd45 proteins have also been implicated in epigenetic gene regulation by promoting active DNA demethylation ...
Yuliya A Sytnikova +4 more
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Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation. [PDF]
PLoS ONE, 2010 Methylation of residues in histone tails is part of a network that regulates gene expression. JmjC domain containing proteins catalyze the oxidative removal of methyl groups on histone lysine residues.
Phillip Hahn +9 more
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