Results 11 to 20 of about 37,245 (219)

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning [PDF]

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +3 more sources

Geographical distribution of Brucella melitensis inferred from rpoB gene variation

Journal of Infection in Developing Countries, 2017
Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for ...
Kim-Kee Tan, Yung-Chie Tan, Li-Yen Chang, Kok Wei Lee, Siti Sarah Nor'e, Wai-Yan Yee, Chee-Choong Hoh, Sazaly AbuBakar   +7 more
doaj   +5 more sources

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH. [PDF]

Journal of Antimicrobial Chemotherapy, 2009
OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment.
Allnutt, Jon, Arnold, Catherine, Bacon, Joanna, Bose, Alpana, Gillespie, Stephen H, Hatch, Kim A, Jenkins, Claire, McHugh, Timothy D, O'Sullivan, Denise M   +8 more
core   +3 more sources

Borderline rpoB mutations transmit at the same rate as common rpoB mutations in a tuberculosis cohort in Bangladesh

Microbial Genomics, 2023
The spread of multidrug-resistant tuberculosis (MDR-TB) is a growing problem in many countries worldwide. Resistance to one of the primary first-line drugs, rifampicin, is caused by mutations in the Mycobacterium tuberculosis rpoB gene. So-called borderline rpoB
Pauline Lempens, Armand Van Deun, Kya J. M. Aung, Mohammad A. Hossain, Mahboobeh Behruznia, Tom Decroo, Leen Rigouts, Bouke C. de Jong, Conor J. Meehan   +8 more
openaire   +3 more sources

The rpoB gene of Mycobacterium tuberculosis [PDF]

Antimicrobial Agents and Chemotherapy, 1994
A portion of the Mycobacterium tuberculosis gene encoding the beta subunit of RNA polymerase (rpoB) was amplified by PCR using degenerate oligonucleotides and used as a hybridization probe to isolate plasmid clones carrying the entire rpoB gene of M. tuberculosis H37Rv, a virulent, rifampin-susceptible strain.
L P, Miller, J T, Crawford, T M, Shinnick   +2 more
openaire   +2 more sources

rpoB sequence-based identification of Mycobacterium avium complex species [PDF]

Microbiology, 2008
The Mycobacterium avium complex (MAC) comprises slowly growing mycobacteria responsible for opportunistic infections and zoonoses. The ability to speciate MAC isolates in the clinical microbiology laboratory is critical for determining the organism implicated in clinical disease and for epidemiological investigation of the source of infection ...
Iskandar, Ben Salah, Toidi, Adékambi, Didier, Raoult, Michel, Drancourt   +3 more
openaire   +2 more sources

rpoB Mutations and Effects on Rifampin Resistance in Mycobacterium tuberculosis

Infection and Drug Resistance, 2021
To investigate the mutations within the whole rpoB gene of Mycobacterium tuberculosis and analyze their effects on rifampin (RIF) resistance based on crystal structure.We sequenced the entire rpoB gene in 175 tuberculosis isolates and quantified their minimum inhibitory concentrations using microplate-based assays.
Ma-chao Li, Jie Lu, Yao Lu, Tong-yang Xiao, Hai-can Liu, Shi-qiang Lin, Da Xu, Gui-lian Li, Xiu-qin Zhao, Zhi-guang Liu, Li-li Zhao, Kang-lin Wan   +11 more
openaire   +5 more sources

rpoB Sequence Analysis of Cultured Tropheryma whippelii [PDF]

Journal of Clinical Microbiology, 2001
ABSTRACT Until recently no isolate of Tropheryma whippelii was available, and therefore genetic studies were limited to those based on PCR amplification of conserved genes. In this study we determined the nucleotide sequence of rpoB (encoding the β-subunit of RNA polymerase) from a ...
M, Drancourt, A, Carlioz, D, Raoult
openaire   +2 more sources

Cloning Reference Gene rpoB in Serratia plymuthica UBCF_13

IOP Conference Series: Earth and Environmental Science, 2021
Abstract The rpoB gene is one of the housekeeping genes in the Serratia plymuthica UBCF_13. This gene is usually used as a reference gene in qPCR analysis to quantify the expression level of the target gene. In the absolute analysis, the reference gene qPCR was cloned to create a standard curve for calculating the copy number of the ...
R Trivano, R Fatiah, J Jamsari
openaire   +1 more source

Detection of rpoB gene mutations in Mycobacterium tuberculosis

Journal of Applied Health Management and Technology, 2023
Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi ...
Widodo Widodo, Ahmad Riadi, Devi Etevia Purlinda, Lilik Setyowatiningsih, Roni Roniafriansya   +4 more
openaire   +1 more source