Results 11 to 20 of about 38,542 (258)

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB and nested-rpoB PCR Cloning [PDF]

Frontiers in Microbiology, 2015
Present study was aimed to examine the diagnostic utility of PCR and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB).
Hossein eMeghdadi, Azar Dokht eKhosravi, Ata eGhadiri, Ameneh eAlami, Amir Hossein eSina   +4 more
doaj   +3 more sources

Geographical distribution of Brucella melitensis inferred from rpoB gene variation

Journal of Infection in Developing Countries, 2017
Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for ...
Kim-Kee Tan, Yung-Chie Tan, Li-Yen Chang, Kok Wei Lee, Siti Sarah Nor'e, Wai-Yan Yee, Chee-Choong Hoh, Sazaly AbuBakar   +7 more
doaj   +5 more sources

Detection of rpoB gene mutations in Mycobacterium tuberculosis [PDF]

Journal of Applied Health Management and Technology, 2023
Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi ...
Widodo Widodo, Ahmad Riadi, Devi Etevia Purlinda, Lilik Setyowatiningsih, Roni Roniafriansya   +4 more
openalex   +2 more sources

Disputed rpoB Mutations in Mycobacterium tuberculosis and Tuberculosis Treatment Outcomes

Antimicrobial Agents and Chemotherapy, 2021
Discordant results between genotypic drug susceptibility testing (gDST) and phenotypic DST (pDST) for Mycobacterium tuberculosis isolates with disputed mutations affect rifampin (RIF)-resistant (RR) and multidrug-resistant (MDR) tuberculosis (TB) treatments due to a lack of practical clinical guidelines.
Wan-Hsuan Lin, Wei-Ting Lee, Hsing-Yuan Tsai, Ruwen Jou   +3 more
openalex   +5 more sources

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH. [PDF]

Journal of Antimicrobial Chemotherapy, 2009
OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment.
Allnutt, Jon, Arnold, Catherine, Bacon, Joanna, Bose, Alpana, Gillespie, Stephen H, Hatch, Kim A, Jenkins, Claire, McHugh, Timothy D, O'Sullivan, Denise M   +8 more
core   +3 more sources

Borderline rpoB mutations transmit at the same rate as common rpoB mutations in a tuberculosis cohort in Bangladesh

Microbial Genomics, 2023
The spread of multidrug-resistant tuberculosis (MDR-TB) is a growing problem in many countries worldwide. Resistance to one of the primary first-line drugs, rifampicin, is caused by mutations in the Mycobacterium tuberculosis rpoB gene. So-called borderline rpoB
Pauline Lempens, Armand Van Deun, Kya J. M. Aung, Mohammad A. Hossain, Mahboobeh Behruznia, Tom Decroo, Leen Rigouts, Bouke C. de Jong, Conor J. Meehan   +8 more
openaire   +3 more sources

Genotyping of the genus Proteus by rpoB sequence analysis

Italian Journal of Public Health, 2005
Background. The genetic relationship among different species within the genus Proteus has still not beenclarified and previous studies by rRNA gene restriction patterns (ribotypes) suggested a high geneticvariability in the presently recognised nomenspecies.Methods.
Giuseppe Giammanco, Sarina Pignato, Patrick A. D. Grimont, Francine Grimont, Anna Giammanco   +4 more
openalex   +6 more sources

The rpoB gene of Mycobacterium tuberculosis [PDF]

Antimicrobial Agents and Chemotherapy, 1994
A portion of the Mycobacterium tuberculosis gene encoding the beta subunit of RNA polymerase (rpoB) was amplified by PCR using degenerate oligonucleotides and used as a hybridization probe to isolate plasmid clones carrying the entire rpoB gene of M. tuberculosis H37Rv, a virulent, rifampin-susceptible strain.
L P, Miller, J T, Crawford, T M, Shinnick   +2 more
openaire   +2 more sources

Haloglomus irregulare gen. nov., sp. nov., a New Halophilic Archaeon Isolated from a Marine Saltern [PDF]

, 2020
A halophilic archaeal strain, designated F16-60T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates.
Durán Viseras, Ana, Sánchez-Porro Álvarez, Cristina, Ventosa Ucero, Antonio   +2 more
core   +1 more source

rpoB sequence-based identification of Mycobacterium avium complex species [PDF]

Microbiology, 2008
The Mycobacterium avium complex (MAC) comprises slowly growing mycobacteria responsible for opportunistic infections and zoonoses. The ability to speciate MAC isolates in the clinical microbiology laboratory is critical for determining the organism implicated in clinical disease and for epidemiological investigation of the source of infection ...
Iskandar, Ben Salah, Toidi, Adékambi, Didier, Raoult, Michel, Drancourt   +3 more
openaire   +2 more sources