rpoB Mutations and Effects on Rifampin Resistance in Mycobacterium tuberculosis
Infection and Drug Resistance, 2021 To investigate the mutations within the whole rpoB gene of Mycobacterium tuberculosis and analyze their effects on rifampin (RIF) resistance based on crystal structure.We sequenced the entire rpoB gene in 175 tuberculosis isolates and quantified their minimum inhibitory concentrations using microplate-based assays.
Ma-chao Li +11 more
openaire +5 more sources
rpoB Sequence Analysis of Cultured Tropheryma whippelii [PDF]
Journal of Clinical Microbiology, 2001 ABSTRACT Until recently no isolate of Tropheryma whippelii was available, and therefore genetic studies were limited to those based on PCR amplification of conserved genes. In this study we determined the nucleotide sequence of rpoB (encoding the β-subunit of RNA polymerase) from a ...
M, Drancourt, A, Carlioz, D, Raoult
openaire +2 more sources
Cloning Reference Gene rpoB in Serratia plymuthica UBCF_13
IOP Conference Series: Earth and Environmental Science, 2021 Abstract The rpoB gene is one of the housekeeping genes in the Serratia plymuthica UBCF_13. This gene is usually used as a reference gene in qPCR analysis to quantify the expression level of the target gene. In the absolute analysis, the reference gene qPCR was cloned to create a standard curve for calculating the copy number of the ...
R Trivano, R Fatiah, J Jamsari
openaire +1 more source
Systematic identification of gene families for use as markers for phylogenetic and phylogeny- driven ecological studies of bacteria and archaea and their major subgroups [PDF]
, 2013 With the astonishing rate that the genomic and metagenomic sequence data sets are accumulating, there are many reasons to constrain the data analyses.
Eisen, Jonathan A. +2 more
core +10 more sources
Genotypic and phenotypic characteristics of Cronobacter species, with particular attention to the newly reclassified species C. helveticus, C. pulveris, and C. zurichensis [forthcoming] [PDF]
, 2014 In 2013, Enterobacter helveticus, E. pulveris and E. turicensis, were reclassified as Cronobacter helveticus, C. pulveris and C. zurichensis, respectively. Previously these species had been used as negative controls for some Cronobacter detection assays.
Forsythe, SJ +3 more
core +1 more source
Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling. [PDF]
, 2019 Light initiates chloroplast biogenesis by activating photosynthesis-associated genes encoded by not only the nuclear but also the plastidial genome, but how photoreceptors control plastidial gene expression remains enigmatic.
Blaha, Gregor M +6 more
core +2 more sources
Four decades of transmission of a multidrug-resistant Mycobacterium tuberculosis outbreak strain [PDF]
, 2015 The rise of drug-resistant strains is a major challenge to containing the tuberculosis (TB) pandemic. Yet, little is known about the extent of resistance in early years of chemotherapy and when transmission of resistant strains on a larger scale became a
Balloux, Francois +6 more
core +4 more sources
Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt [PDF]
, 2019 Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their
El-Adawy, Hosny +9 more
core +1 more source
rpoB Mutation Conferring Rifampin Resistance in Streptococcus pyogenes [PDF]
Antimicrobial Agents and Chemotherapy, 2002 ABSTRACT Streptococcus pyogenes BM4478 and Staphylococcus aureus BM4479 were isolated from a patient undergoing rifampin therapy. High-level resistance to rifampin was due to the following mutations in the rpoB gene: Ser 522 Leu in strain BM4478 ...
Hélène, Aubry-Damon +3 more
openaire +2 more sources
Journal of Pure and Applied Microbiology, 2019 Anti-tubercular therapy is one of the effective strategies used to control tuberculosis, so, a planned and accurate treatment regimen is of utmost importance, but number of cases are being treated as MDR on the basis of rifampicin mono resistance.
Sana Nudrat, Umar Farooq, Mazhar Maqsood
doaj +1 more source