Results 41 to 50 of about 52,178 (281)

INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY [PDF]

Biotechnologia Acta, 2017
Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.
O.V. Galkin, T.O. Chudina, A.A. Siromolot, O.S. Oliynyk   +3 more
doaj   +1 more source

Nanobody based dual specific CARs [PDF]

, 2018
Recent clinical trials have shown that adoptive chimeric antigen receptor (CAR) T cell therapy is a very potent and possibly curative option in the treatment of B cell leukemias and lymphomas.
Abken, Hinrich, Bonte, Sarah, De Munter, Stijn, Goetgeluk, Glenn, Ingels, Joline, Kerre, Tessa, Pille, Melissa, Vandekerckhove, Bart, Weening, Karin   +8 more
core   +2 more sources

Deletion of parasite immune modulatory sequences combined with immune activating signals enhances vaccine mediated protection against filarial nematodes [PDF]

, 2012
<p>Background: Filarial nematodes are tissue-dwelling parasites that can be killed by Th2-driven immune effectors, but that have evolved to withstand immune attack and establish chronic infections by suppressing host immunity. As a consequence, the
AB Fredriksen, AB Pedersen, AE O'Connell, AE Schwab, B de Andres, B Manoury, BL Makepeace, BW Li, C Klotz, C Martin, C Martin, D Abraham, D Abraham, D Horst, D Knox, David W. Taylor, DH Barouch, DL Redmond, E Maraskovsky, G Ahlen, G Nchinda, GT Joseph, HA Farid, HongLin Luo, J Gardon, J Murray, JE Allen, JE Allen, JJ Donnelly, JP Hewitson, Judith E. Allen, KJ Fairlie-Clarke, KJ Ishii, LM Mir, LM Mir, LvG Jørgensen, MD Taylor, MD Taylor, MY Osei-Atweneboana, N Gomez-Escobar, Nick Gray, O Golden, PJ Hotez, R Ihaka, RM Maizels, RT Rwegoshora, S Babayan, S Frontier, S Mendez, S Ramachandran, S Thirugnanam, SA Babayan, SA Babayan, SB Anand, SB Anand, SB Anand, Simon A. Babayan, SM Sumida, Stephen John Davies, W Harnett, WF Gregory, WF Gregory, Y Dash, Y Wu   +63 more
core   +4 more sources

Effects of Cultivation Conditions on the Expression Level of Recombinant scFv Antibody against EpEX in Escherichia coli

Trends in Peptide and Protein Sciences, 2019
The EpCAM (epithelial cell adhesion molecule) is a cell surface antigen over expressed in many types of epithelial cell cancers including colon, stomach, pancreas, lung, ovarian, and breast.
Seyyed ali Mohammadi Vadoud, Atieh Hashemi, Malihe keramati, Farideh Rasouli Kery Bozorg   +3 more
doaj   +1 more source

Positron emission tomography imaging of endometrial cancer using engineered anti-EMP2 antibody fragments. [PDF]

, 2012
PurposeAs imaging of the cell surface tetraspan protein epithelial membrane protein-2 (EMP2) expression in malignant tumors may provide important prognostic and predictive diagnostic information, the goal of this study is to determine if antibody ...
Braun, Jonathan, Brewer, Sarah, Fu, Maoyong, Gordon, Lynn K, Olafsen, Tove, Said, Jonathan, Wadehra, Madhuri, Wu, Anna M   +7 more
core   +3 more sources

Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells

Human Vaccines & Immunotherapeutics, 2018
The aim of this study was to produce a humanized single chain antibody (scFv) as a potential improved product design to target EGFR (Epidermal Growth Factor Receptor) overexpressing cancer cells.
Arsham Banisadr, Yaghoub Safdari, Anvarsadat Kianmehr, Mahdieh Pourafshar   +3 more
doaj   +1 more source

Fusion Expression and Affinity Identification of Two scFvs Against CEA or CA19-9 in HEK293 Cells

Zhongliu Fangzhi Yanjiu, 2019
Objective To express two scFv-Fc fusion proteins of anti-CEA scFv-Fc and anti-CA19-9 scFv-Fc in HEK293 cells efficiently, and to determinate the affinity of two single chain antibodies. Methods Two scFv gene sequences obtained from GenBank were optimized
LU Wenbin, GONG Xuechao, JIN Jianhua, HU Wenwei, WANG Yue, ZHANG Hua   +5 more
doaj   +1 more source

A modular IgG-scFv bispecific antibody topology [PDF]

Protein Engineering, Design and Selection, 2009
Here we present a bispecific antibody (bsAb) format in which a disulfide-stabilized scFv is fused to the C-terminus of the light chain of an IgG to create an IgG-scFv bifunctional antibody. When expressed in mammalian cells and purified by one-step protein A chromatography, the bsAb retains parental affinities of each binding domain, exhibits IgG-like ...
Kelly Davis, Orcutt, Margaret E, Ackerman, Maryelise, Cieslewicz, Emmanuel, Quiroz, Adrian L, Slusarczyk, John V, Frangioni, K Dane, Wittrup   +6 more
openaire   +2 more sources

RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

BMC Cancer, 2021
Background We prepared an anti-p21Ras scFv which could specifically bind with mutant and wild-type p21Ras. However, it cannot penetrate the cell membrane, which prevents it from binding to p21Ras in the cytoplasm.
Chen-Chen Huang, Fang-Rui Liu, Qiang Feng, Xin-Yan Pan, Shu-Ling Song, Ju-Lun Yang   +5 more
doaj   +1 more source

Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. [PDF]

, 2018
DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes in DNA methylation can be heritable and, thus, can lead to the formation of stable epialleles. A well-characterized example of
Gallego-Bartolomé, Javier, Gardiner, Jason, Ghoshal, Basudev, Jacobsen, Steven E, Kuo, Hsuan Yu, Liu, Wanlu, Papikian, Ashot, Segal, David J, Zhao, Jenny Miao-Chi   +8 more
core   +2 more sources