Results 61 to 70 of about 52,178 (281)

Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production [PDF]

, 2019
Although many recombinant proteins have been produced in seeds at high yields without adverse effects on the plant, endoplasmic reticulum (ER) stress and aberrant localization of endogenous or recombinant proteins have also been reported.
Arcalis, Elsa, Avesani, Linda, Bortesi, Luisa, De Meyer, Thomas, de meyer, thomas, Depicker, Anna, Hilscher, Julia, Ibl, Verena, Morandini, Francesca, Pezzotti, Mario, Pum, Dietmar, Rademacher, Thomas, Stoger, Eva, Vitale, Alessandro   +13 more
core   +2 more sources

A strategy for adenovirus vector targeting with a secreted single chain antibody. [PDF]

PLoS ONE, 2009
BACKGROUND:Successful gene therapy will require targeted delivery vectors capable of self-directed localization. In this regard, the use of antibodies or single chain antibody fragments (scFv) in conjunction with adenovirus (Ad) vectors remains an ...
Joel N Glasgow, Galina Mikheeva, Victor Krasnykh, David T Curiel   +3 more
doaj   +1 more source

Development of an Anti-HER2 Single-Chain Variable Antibody Fragment Construct for High-Yield Soluble Expression in Escherichia coli and One-Step Chromatographic Purification

Biomolecules, 2023
Although single-chain variable fragment (scFv) is recognized as a highly versatile scaffold of recombinant antibody fragment molecules, its overexpression in Escherichia coli often leads to the formation of inclusion bodies.
Kyu Tae Byun, Boram Kim, Junmin Cho, Inbeom Lee, Myung Gu Lee, Dongsun Park, Tae-Bong Kang, Hyung-Sik Won, Chan Gil Kim   +8 more
doaj   +1 more source

Integrated scFv identification and CAR T cell generation for AML targeting in vivo. [PDF]

Int J Cancer
What's new? Acute myeloid leukemia (AML) is a rapidly progressing blood cancer. Advancing chimeric antigen receptor (CAR) T cell therapies, particularly through rapid translation of single chain variable fragment (scFv) discovery into CAR constructs, may improve clinical outcomes, especially the development of patient individualized CAR T cells.
Liu Y, Lauk A, Sedloev D, Brysting J, Cetin E, Liu C, Mönnig M, Luft T, Yun H, Schmitt M, Sauer T, Zhou F, Rohde C, Müller-Tidow C.   +13 more
europepmc   +2 more sources

Enhanced transport of plant-produced rabies single chain antibody-RVG peptide fusion protein across an in cellulo blood-brain barrier device [PDF]

, 2017
The biomedical applications of antibody engineering are developing rapidly and have been expanded to plant expression platforms. In this study, we have generated a novel antibody molecule in planta for targeted delivery across the blood–brain barrier ...
Al-Shehri, Alyautdin, Alyautdin, Bird, Boado, Both, Bracci, Burrage, Chopy, Donnelly-Roberts, Eigenmann, Fooks, Friden, Fu, Greene, Gulyaev, Hefferon, Helm, Hemachudha, Hwang do, Jekic, Kim, Kohli, Kumar, Kwon, Lentz, Lentz, Lentz, Lentz, Lentz, Lentz, Lewis, Lim, Liu, Louie, Ma, Markoutsa, McQuarrie, Megret, Muller, Pardridge, Pardridge, Pardridge, Peyret, Rosales-Mendoza, Roy, Rustici, Sainsbury, Schwarze, Shahid, Shantavasinkul, Siflinger-Birnboim, Thoulouze, Tzartos, Uwanyiligira, Xiang, Yamauchi   +56 more
core   +1 more source

Effective single chain antibody (scFv) concentrations in vivo via adenoviral vector mediated expression of secretory scFv [PDF]

Gene Therapy, 2002
Single chain antibodies (scFv) represent powerful interventional agents for the achievement of targeted therapeutics. The practical utility of these agents have been limited, however, by difficulties related to production of recombinant scFv and the achievement of effective and sustained levels of scFv in situ.
W O, Arafat, J, Gómez-Navarro, D J, Buchsbaum, J, Xiang, M, Wang, E, Casado, S D, Barker, P J, Mahasreshti, H J, Haisma, M N, Barnes, G P, Siegal, R D, Alvarez, A, Hemminki, D M, Nettelbeck, D T, Curiel   +14 more
openaire   +3 more sources

Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. [PDF]

, 2018
Oxidized phospholipids (OxPL) are ubiquitous, are formed in many inflammatory tissues, including atherosclerotic lesions, and frequently mediate proinflammatory changes 1 .
Binder, Christoph J, Bowden, Karen, Diehl, Cody, Gaddis, Dalia E, Glass, Christopher K, Gonen, Ayelet, Hedrick, Catherine C, Hung, Ming-Yow, Ley, Klaus, MacDonald, Jeffrey G, Mellon, Pamela L, Miller, Yury I, Määttä, Antti, Pattison, Jennifer, Peterson, Kirk L, Prohaska, Thomas A, Que, Xuchu, Sun, Xiaoli, Tsimikas, Sotirios, Witztum, Joseph L, Yeang, Calvin, Ylä-Herttuala, Seppo   +21 more
core   +1 more source

Intein‐based modular chimeric antigen receptor platform for specific CD19/CD20 co‐targeting

Molecular Oncology, EarlyView.
CARtein is a modular CAR platform that uses split inteins to splice antigen‐recognition modules onto a universal signaling backbone, enabling precise, scarless assembly without re‐engineering signaling domains. Deployed here against CD19 and CD20 in B‐cell malignancies, the design supports flexible multi‐antigen targeting to boost T‐cell activation and
Pablo Gonzalez‐Garcia, Noelia Moares, Wenjie Yi‐He, Rosa Luna‐Espejo, Ricardo Fernandez‐Cisnal, Javier Ocaña‐Cuesta, Juan P. Muñoz‐Miranda, Antonio Gabucio, Cecilia M. Fernandez‐Ponce, Francisco Garcia‐Cozar   +9 more
wiley   +1 more source

Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer's disease

Theranostics, 2019
Investigation of targeting inhibitors of Aβ aggregation, heme-Aβ peroxidase-like activity and efficient detectors of Aβ aggregation, are of therapeutic value and diagnostics significance for the treatment of Alzheimer's disease (AD).
Dongni Liu, Wei Li, Xiaoyu Jiang, S. Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, W. Kong, R. Gao, Yaming Shan   +10 more
semanticscholar   +1 more source

Recombinant single chain variable fragment antibodies (scFv) against Pro(144)-Leu(155) fragment of human protein C [PDF]

The Ukrainian Biochemical Journal, 2015
The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein
O. S. Oliinyk, K. O. Palyvoda, N. E. Lugovskaya, D. V. Kolibo, E. V. Lugovskoy, S. V. Komisarenko   +5 more
doaj   +1 more source