Expression Kinetics and Innate Immune Response after Electroporation and LNP-Mediated Delivery of a Self-Amplifying mRNA in the Skin [PDF]
Molecular Therapy: Nucleic Acids, 2019 In this work, we studied the expression kinetics and innate immune response of a self-amplifying mRNA (sa-RNA) after electroporation and lipid-nanoparticle (LNP)-mediated delivery in the skin of mice. Intradermal electroporation of the sa-RNA resulted in
Hanne Huysmans +8 more
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Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccines [PDF]
Molecular Therapy: Nucleic Acids, 2023 To be effective, RNA vaccines require both in situ translation and the induction of an immune response to recruit cells to the site of immunization. These factors can pull in opposite directions with the inflammation reducing expression of the vaccine ...
John S. Tregoning +13 more
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Self-Amplifying RNA Vaccine Candidates: Alternative Platforms for mRNA Vaccine Development [PDF]
Pathogens, 2023 The present use of mRNA vaccines against COVID-19 has shown for the first time the potential of mRNA vaccines for infectious diseases. Here we will summarize the current knowledge about improved mRNA vaccines, i.e., the self-amplifying mRNA (saRNA ...
Christin Schmidt, Barbara S. Schnierle
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A self-amplifying RNA vaccine provides protection in a murine model of bubonic plague [PDF]
Frontiers in Microbiology, 2023 Mice were immunized with a combination of self-amplifying (sa) RNA constructs for the F1 and V antigens of Yersinia pestis at a dose level of 1 μg or 5 μg or with the respective protein sub-units as a reference vaccine.
Robin John Shattock +11 more
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A bivalent self-amplifying RNA vaccine against yellow fever and Zika viruses [PDF]
Frontiers in ImmunologyIntroductionYellow fever (YFV) and Zika (ZIKV) viruses cause significant morbidity and mortality, despite the existence of an approved YFV vaccine and the development of multiple ZIKV vaccine candidates to date.
Peter Battisti +19 more
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Self-Amplifying RNA: Advantages and Challenges of a Versatile Platform for Vaccine Development [PDF]
VirusesSelf-amplifying RNA is synthetic nucleic acid engineered to replicate within cells without generating viral particles. Derived from alphavirus genomes, saRNA retains the non-structural elements essential for replication while replacing the structural ...
Thomas Vallet, Marco Vignuzzi
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A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stability [PDF]
npj Vaccines, 2022 mRNA vaccines were the first to be authorized for use against SARS-CoV-2 and have since demonstrated high efficacy against serious illness and death. However, limitations in these vaccines have been recognized due to their requirement for cold storage ...
Emily A. Voigt +14 more
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Self-Amplifying RNA: A Second Revolution of mRNA Vaccines against COVID-19 [PDF]
VaccinesSARS-CoV-2 virus, the causative agent of COVID-19, has produced the largest pandemic in the 21st century, becoming a very serious health problem worldwide.
Noelia Silva-Pilipich +3 more
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Enhanced immune responses following heterologous vaccination with self-amplifying RNA and mRNA COVID-19 vaccines. [PDF]
PLoS Pathogens, 2022 The optimal vaccination strategy to boost responses in the context of pre-existing immune memory to the SARS-CoV-2 spike (S) glycoprotein is an important question for global public health.
Tamara Elliott +13 more
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A sort and sequence approach to dissect heterogeneity of response to a self-amplifying RNA vector in a novel human muscle cell line [PDF]
Molecular Therapy: Nucleic AcidsSelf-amplifying RNA (saRNA) is an extremely promising platform because it can produce more protein for less RNA. We used a sort and sequence approach to identify host cell factors associated with transgene expression from saRNA; the hypothesis was that ...
Rachel D. Barton +6 more
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