Results 31 to 40 of about 7,535 (227)

Room Temperature Serial Crystallography at Synchrotrons [PDF]

Acta Crystallographica Section A Foundations and Advances, 2014
Serial Femtosecond Crystallography (SFX) is the most commonly used method for the emerging structure determination at X-ray free-electron lasers (FELs). The high peak brilliance of the FEL and the possibility of using femtosecond pulses afford use of nano-to-micron sized crystals in a diffraction-before-destruction approach for the acquisition of high ...
Sabine Botha, Thomas Barends, Wolfgang Kabsch, Beatrice Latz, Karol Nass, Robert Shoeman, Florian Dworkowski, Ezequiel Panepucci, Meitian Wang, Ilme Schlichting, R. Bruce Doak   +10 more
openaire   +1 more source

Towards the spatial resolution of metalloprotein charge states by detailed modeling of XFEL crystallographic diffraction. [PDF]

, 2020
Oxidation states of individual metal atoms within a metalloprotein can be assigned by examining X-ray absorption edges, which shift to higher energy for progressively more positive valence numbers.
Holton, James M, Kern, Jan, Sauter, Nicholas K, Yano, Junko   +3 more
core   +2 more sources

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals. [PDF]

, 2020
Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful ...
Aquila, Andrew, Bhowmick, Asmit, Biel, Justin T, Boutet, Sebastien, Brewster, Aaron S, Carbajo, Sergio, Cohen, Aina E, Cortez, Saul, Fraser, James S, Gonen, Tamir, Gonzalez, Ana, Hino, Tomoya, Im, Dohyun, Ito, Kazutaka, Iwata, So, Koralek, Jake D, Kubo, Minoru, Lazarou, Tomas S, Martynowycz, Michael W, Nakane, Takanori, Nango, Eriko, Nomura, Takashi, Owada, Shigeki, Samelson, Avi J, Sauter, Nicholas K, Sierra, Raymond G, Sugahara, Michihiro, Tanaka, Rie, Tanaka, Tomoyuki, Thompson, Erin M, Thompson, Michael C, Tono, Kensuke, van den Bedem, Henry, Woldeyes, Rahel A, Wolff, Alexander M, Young, Iris D, Yumoto, Fumiaki, Zhao, Wei   +37 more
core   +2 more sources

A split-beam probe-pump-probe scheme for femtosecond time resolved protein X-ray crystallography [PDF]

, 2015
In order to exploit the femtosecond pulse duration of X-ray Free-Electron Lasers (XFEL) operating in the hard X-ray regime for ultrafast time-resolved protein crystallography experiments, critical parameters that determine the crystallographic signal-to ...
Madsen, A, Van Thor, JJ
core   +4 more sources

Serial crystallography at synchrotrons and X-ray lasers [PDF]

IUCrJ, 2017
Serial crystallography was developed for the use at free-electron lasers but the approach has recently also been adapted to synchrotron sources. Here we discuss how the synergy between the two X-ray sources will facilitate a wide application of the technique in microcrystallography, room-temperature structure determination and time-resolved studies.
Jörg Standfuss, John Spence
openaire   +4 more sources

Macromolecular Serial Crystallography (Volume II)

Crystals, 2022
The successful adaptation of the serial macromolecular crystallography approach at most 3rd generation synchrotron facilities allows a fruitful synergy between synchrotrons and XFELs that have accelerated the access and impact of this approach to an even
Jose M. Martin-Garcia
doaj   +1 more source

Fixed-Target Serial Synchrotron Crystallography Using Nylon Mesh and Enclosed Film-Based Sample Holder

Crystals, 2020
Serial crystallography (SX) technique using synchrotron X-ray allows the visualization of room-temperature crystal structures with low-dose data collection as well as time-resolved molecular dynamics.
Suk-Youl Park, Hyeongju Choi, Cheolsoo Eo, Yunje Cho, Ki Hyun Nam   +4 more
doaj   +1 more source

Time-resolved β-lactam cleavage by L1 metallo-β-lactamase

Nature Communications, 2022
Metallo-β-lactamases cleave β-lactam moiety of many broadly used antibiotics. Here the authors captured mechanistic details of the enzyme catalyzed reaction using time-resolved xray synchrotron serial crystallography.
M. Wilamowski, D. A. Sherrell, Y. Kim, A. Lavens, R. W. Henning, K. Lazarski, A. Shigemoto, M. Endres, N. Maltseva, G. Babnigg, S. C. Burdette, V. Srajer, A. Joachimiak   +12 more
doaj   +1 more source

Serial synchrotron crystallography for time-resolved structural biology

Current Opinion in Structural Biology, 2020
The current state-of-the-art experiments in time-resolved structural biology are undoubtedly the recent extremely impressive results that are emerging from XFEL-based experiments. However, there is a large range of macromolecular systems where the biological interest is predominantly in the slower dynamics (μs-s), that produce well diffracting ...
Pearson, A., Mehrabi, P.
openaire   +3 more sources

Protein Dynamics and Time Resolved Protein Crystallography at Synchrotron Radiation Sources: Past, Present and Future

Crystals, 2021
The ultrabright and ultrashort pulses produced at X-ray free electron lasers (XFELs) has enabled studies of crystallized molecular machines at work under ‘native’ conditions at room temperature by the so-called time-resolved serial femtosecond ...
Jose M. Martin-Garcia
doaj   +1 more source