Purification and biochemical characterization of a vacuolar serine endopeptidase induced by glucose starvation in maize roots [PDF]
Biochemical Journal, 1996 An endopeptidase (designated RSIP, for root-starvation-induced protease) was purified to homogeneity from glucose-starved maize roots. The molecular mass of the enzyme was 59 kDa by SDS/PAGE under reducing conditions and 62 kDa by gel filtration on a Sephacryl S-200 column. The isoelectric point of RSIP was 4.55. The purified enzyme was stable, with no
Franck JAMES +4 more
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Journal of Biological Chemistry, 1991 The present work describes the detection, purification, and characterization of a serine endopeptidase with preference for a phosphoserine in the P1' position of the substrate. During probing for the enzyme in crude extracts, as well as during its 64,000-fold purification, 32P-labeled guanidovaleryl-Arg-Ala-Ser(P)-isobutyl amide (I) was used to measure
Jonathan Rosen +3 more
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Specific fluorogenic substrates for neprilysin (neutral endopeptidase, EC 3.4.24.11) which are highly resistant to serine- and metalloproteases [PDF]
Brazilian Journal of Medical and Biological Research, 1997 Two intramolecularly quenched fluorogenic peptides containing o-aminobenzoyl (Abz) and ethylenediamine 2,4-dinitrophenyl (EDDnp) groups at amino- and carboxyl-terminal amino acid residues, Abz-DArg-Arg-Leu-EDDnp (Abz-DRRL-EDDnp) and Abz-DArg-Arg-Phe-EDDnp (Abz-DRRF-EDDnp), were selectively hydrolyzed by neutral endopeptidase (NEP, enkephalinase ...
Marco Alberto Medeiros +4 more
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Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system inPorphyromonas gingivalis [PDF]
FEMS Microbiology Letters, 2014 Porphyromonas gingivalis, a significant causative agent of adult periodontitis, possesses a novel secretion system called the type IX secretion system (T9SS). A number of virulence factors, such as Arg-gingipain (Rgp), are translocated onto the cell surface and into the extracellular milieu via the T9SS. In this study, we found that the PGN_1416 90- to
Minako Nonaka +6 more
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Novel features of serine protease active sites and specificity pockets: sequence analysis and modelling studies of glutamate-specific endopeptidases and epidermolytic toxins [PDF]
Protein Engineering, 1996 João Alexandra +3 more
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Biological Chemistry, 2001 Infection by Staphylococcus epidermidis, an opportunistic pathogen, has become a major problem due to the increased use of implanted medical devices and the growing number of patients who are therapeutically or infectiously immunosuppressed. These infections appear to proceed via modulation of the coagulation and complement systems.
J Moon +4 more
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Sensitive substrates for neprilysin (neutral endopeptidase) and thermolysin that are highly resistant to serine proteases [PDF]
FEBS Letters, 1996 Tripeptide derivatives like 3‐carboxypropanoylalanyl‐alanyl‐leucine 4‐nitroanilide or 3‐carboxypropanoylalanyl‐alanyl‐phenylalanine 4‐nitroanilide are very sensitive substrates for neprilysin (k cat > 102 s−1; k cat/K m ≥ 106 s−1 · M−1) and are widely employed in investigations of the enzyme. However, these compounds are also good substrates for the
A. Spungin‐Bialik +4 more
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Journal of Biological Chemistry, 1992 A prolyl endopeptidase was purified from Flavobacterium meningosepticum. It was digested with trypsin. Two oligonucleotides, based on tryptic peptide sequences and used in PCR experiments, amplified a 300-base pair (bp) fragment. A 2.4-kilobase EcoRI fragment that hybridized to the 300-bp probe was cloned in lambda ZAP and sequenced from both strands ...
Sylvie Chevallier +4 more
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Phosphorylated acidic serine–aspartate-rich MEPE-associated motif peptide from matrix extracellular phosphoglycoprotein inhibits phosphate regulating gene with homologies to endopeptidases on the X-chromosome enzyme activity [PDF]
Journal of Endocrinology, 2007 Shiguang Liu +4 more
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Arterivirus Serine Endopeptidase
, 2012 Eric J. Snijder, Alexander E. Gorbalenya
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