Results 41 to 50 of about 564 (139)

A Class of Shark‐Derived Single‐Domain Antibodies can Broadly Neutralize SARS‐Related Coronaviruses and the Structural Basis of Neutralization and Omicron Escape

Small Methods, Volume 6, Issue 7, July 20, 2022., 2022
Shark‐derived vnarbodies 20G6 and 17F6 broadly neutralize SARS‐CoV‐2 Wuhan, Alpha, Beta, Kappa, Delta, Delta‐plus, Lambda variants, and sarbecovirus coronaviruses, but are unexpectedly ineffective for Omicron. Intranasal administration of 20G6 confers effective preventive and therapeutic effects in mice infected with SARS‐CoV‐2 Wuhan and Beta variant ...
Bo Feng, Zhilong Chen, Jing Sun, Tingting Xu, Qian Wang, Haisu Yi, Xuefeng Niu, Jiabin Zhu, Mengzhu Fan, Ruitian Hou, Ying Shao, Sihui Huang, Cuiyun Li, Peiyu Hu, Pingqian Zheng, Ping He, Jia Luo, Qihong Yan, Xiaoli Xiong, Jinsong Liu, Jincun Zhao, Ling Chen   +21 more
wiley   +1 more source

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications

Cells, 2021
Shark is a cartilaginous fish that produces new antigen receptor (IgNAR) antibodies. This antibody is identified with a similar human heavy chain but dissimilar sequences.
Salma Nassor Juma, Xiaoxia Gong, Sujie Hu, Zhengbing Lv, Jianzhong Shao, Lili Liu, Guiqian Chen   +6 more
doaj   +1 more source

Identification of Anti-TNFα VNAR Single Domain Antibodies from Whitespotted Bambooshark (Chiloscyllium plagiosum)

Marine Drugs, 2022
Tumor necrosis factor α (TNFα), an important clinical testing factor and drug target, can trigger serious autoimmune diseases and inflammation. Thus, the TNFα antibodies have great potential application in diagnostics and therapy fields.
Linfei Zhao, Mingliang Chen, Xiaona Wang, Shoukai Kang, Weiwei Xue, Zengpeng Li   +5 more
doaj   +1 more source

Isolation of highly selective IgNAR variable single-domains against a human therapeutic Fc scaffold and their application as tailor-made bioprocessing reagents [PDF]

, 2021
Funding This work was supported by the Industrial Biotechnology Innovation Centre, and Merck KGaA. Acknowledgements The authors would like to thank Iris Willenbücher and Kerstin Hallstein for the BIAcore™ analysis and Nadine Barron for the bio-layer ...
Barelle, Caroline J, Becker, Stefan, Buschhaus, Magdalena J, Porter, Andrew J   +3 more
core   +1 more source

Marine-derived microbes and molecules for drug discovery

Inflammation and Regeneration, 2022
Increasing attention has been paid to marine-derived biomolecules as sources of therapeutics for autoimmune diseases. Nagasaki Prefecture has many islands and is surrounded by seas, straits, gulfs, bays, and coves, giving it the second longest coastline ...
Yoshimasa Tanaka, Megumi Nishikawa, Kaho Kamisaki, Saki Hachiya, Moeka Nakamura, Takahiro Kuwazuru, Susumu Tanimura, Kiyoshi Soyano, Kohsuke Takeda   +8 more
doaj   +1 more source

Shark-derived single-domain antibodies for the detection of decapod iridescent virus 1

Aquaculture Reports, 2023
Decapod iridescent virus 1 (DIV1) has emerged as a novel threat to shrimp farming. The development of a rapid, convenient, and accurate method for diagnosing DIV1 is urgently needed, serving as a key factor in the reduction of the risk of viral outbreaks.
Jinlin Chen, Wei Wang, Qin Zheng, Yunying Cheng, Qian Zhang, Shenfei Jiang, Jianming Chen   +6 more
doaj   +1 more source

Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD Protein

International Journal of Molecular Sciences, 2022
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is the major target for antibody therapeutics. Shark-derived variable domains of new antigen receptors (VNARs) are the smallest antibody fragments with flexible paratopes that can recognize protein motifs inaccessible to classical antibodies. This study reported four VNARs binders (JM-2,
Yu-Lei Chen, Jin-Jin Lin, Huan Ma, Ning Zhong, Xin-Xin Xie, Yunru Yang, Peiyi Zheng, Ling-Jing Zhang, Tengchuan Jin, Min-Jie Cao   +9 more
openaire   +2 more sources

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity [PDF]

, 2009
Antibodies are important tools for experimental research and medical applications. Most antibodies are composed of two heavy and two light chains. Both chains contribute to the antigen-binding site which is usually flat or concave.
Janusz Wesolowski, Vanina Alzogaray, Jan Reyelt, Mandy Unger, Karla Juarez, Mariela Urrutia, Ana Cauerhff, Welbeck Danquah, Björn Rissiek, Felix Scheuplein, Nicole Schwarz, Sahil Adriouch, Olivier Boyer, Michel Seman, Alexei Licea, David V. Serreze, Fernando A. Goldbaum, Friedrich Haag, Friedrich Koch-Nolte, OH Brekke, C Hamers-Casterman, S Muyldermans, P Holliger, A Desmyter, E De Genst, TT Wu, MM Harmsen, A Pini, HR Hoogenboom, V Cortez-Retamozo, M Dumoulin, EK Conrath, U Rothbauer, A Olichon, K Coppieters, N Pant, D Gueorguieva, SA Jobling, B Serruys, M Feldmann, N Lonberg, A Drucker, JW Goding, M Salmi, D Corda, A Zolkiewska, F Di Virgilio, F Koch-Nolte, S Adriouch, F Koch-Nolte, F Scheuplein, S Adriouch, M Seman, V Ghetie, G Behar, AJ Groot, A Muruganandam, A Abulrob, V Cortez-Retamozo, K Omidfar, R Roovers, L Huang, BM Tijink, F Rahbarizadeh, D Ahmadvand, MA Palladino, DR Maass, W Wolfson, JJ Hulstein, D Schenk, M Dumoulin, P Lafaye, G Habicht, R Klooster, C Cohen-Saidon, C Avignolo, AJ Groot, AJ Groot, P Verheesen, D Laune, A Marquardt, DA Laplagne, LM Zarebski, DP Simmons, T Gura, K Strebhardt, K Aktories, ER Goldman, JL Liu, ER Goldman, M El Khattabi, RA Harrison, F Meddeb-Mouelhi, RA Harrison, CS Stewart, I Hmila, PJ Doyle, S Spinelli, RC Ladenson, GP Anderson, N Alvarez-Rueda, DM Goldenberg, A Forsman, L Garaicoechea, MM Harmsen, MM Harmsen, A Hultberg, HJ de Haard, AM Ledeboer, S Dekker, LJ Sherwood, MM Harmsen, A Szynol, C Krüger, KV Korotkov, AY Lam, S Spinelli, KE Conrath, B Stijlemans, TN Baral, D Saerens, L Ratier, N Deckers, E Dolk, AS Greenberg, C Vincke, X Zou, R Janssens, EG Hofman, LO Gainkam, F Rahbarizadeh, S Winichayakul, D Saerens, D Saerens, PH Chan, A Chartier, S van Koningsbruggen, JL Liu, JM van der Vaart, MM Harmsen, KA Henderson, W DeLano, RL Stanfield, AT Hadfield, BC Braden, MR Bazl, U Rothbauer   +146 more
core   +6 more sources

Brain delivery of biologics using a cross‐species reactive transferrin receptor 1 VNAR shuttle

The FASEB Journal, Volume 34, Issue 10, Page 13272-13283, October 2020., 2020
Abstract Transferrin receptor 1 (TfR1) mediated transcytosis is an attractive strategy to enhance brain uptake of protein drugs, but translation remains a challenge. Here, a single domain shark antibody VNAR fragment (TXB2) with similar affinity to murine and human TfR1 was used to shuttle protein cargo into the brain. TXB2 was fused to a human IgG1 Fc
Dag Sehlin, Pawel Stocki, Tobias Gustavsson, Greta Hultqvist, Frank S. Walsh, J. Lynn Rutkowski, Stina Syvänen   +6 more
wiley   +1 more source

Enhancing the Transdermal Delivery of ‘Next Generation’ Variable New Antigen Receptors Using Microarray Patch Technology: a Proof-of-Concept Study [PDF]

, 2022
Heavy chain only binding proteins, such as variable new antigen receptors (VNARs), have emerged as an alternative to the highly successful therapeutic monoclonal antibodies (mAb).
Barelle, C., Donnelly, R.F., Hutton, A.R.J., Ubah, O.   +3 more
core   +1 more source