The characterization of variable new antigen receptors targeting FAP isolated from a novel immunized library [PDF]
Communications BiologyCancer-associated fibroblasts (CAFs) in the stroma of solid tumors promote an immunosuppressive tumor microenvironment (TME) that drives resistance to therapies.
Gihan S. Gunaratne +15 more
doaj +2 more sources
Theranostic vNAR-Based Immunoconjugates Achieve Selective Intracellular Cisplatin Delivery in Embedded 3D HER2-Positive Breast Cancer In Vitro Model [PDF]
PharmaceuticalsBackground/Objectives: Precise intracellular delivery of chemotherapeutics remains a major challenge in HER2-positive breast cancer, where intratumoral heterogeneity and limited tissue penetration constrain efficacy.
Andrea C. Alfonseca-Ladrón de Guevara +5 more
doaj +2 more sources
A novel shark single-domain antibody targeting OGT as a tool for detection and intracellular localization [PDF]
Frontiers in Immunology, 2023 IntroductionO-GlcNAcylation is a type of reversible post-translational modification on Ser/Thr residues of intracellular proteins in eukaryotic cells, which is generated by the sole O-GlcNAc transferase (OGT) and removed by O-GlcNAcase (OGA).
Xiaozhi Xi +21 more
doaj +2 more sources
Shark IgNAR: The Next Broad Application Antibody in Clinical Diagnoses and Tumor Therapies? [PDF]
Marine Drugs, 2023 Antibodies represent a relatively mature detection means and serve as therapeutic drug carriers in the clinical diagnosis and treatment of cancer—among which monoclonal antibodies (mAbs) currently occupy a dominant position.
Xiaofeng Jiang +5 more
doaj +2 more sources
Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF165 Neutralization
Marine Drugs, 2018 Variable new antigen receptor domain (vNAR) antibodies are novel, naturally occurring antibodies that can be isolated from naïve, immune or synthetic shark libraries. These molecules are very interesting to the biotechnology and pharmaceutical industries
Tanya A Camacho-Villegas +2 more
exaly +3 more sources
Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library [PDF]
Malaria Journal, 2018 Background Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (
Chiuan Herng Leow +5 more
doaj +6 more sources
Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark [PDF]
Marine DrugsHeavy chain-only antibodies in sharks are called immunoglobulin new antigen receptors (IgNAR), consisting of one variable region (VNAR) and five constant regions (C1-C5).
Reo Uemura +8 more
doaj +2 more sources
Humanization of the Shark V<sub>NAR</sub> Single Domain Antibody Using CDR Grafting. [PDF]
Curr Protoc, 2023 AbstractThe variable domain of the new antigen receptor (VNAR) of shark single domain antibodies is evolutionarily distant from the variable regions (VH) of mammalian immunoglobulins, yet it still has complementarity‐determining regions (CDRs) that are involved in antigen recognition, therefore making it possible to humanize by grafting these CDRs to ...
Zhang YF, Sun Y, Hong J, Ho M.
europepmc +3 more sources
Improving the pharmacokinetic properties of biologics by fusion to an anti-HSA shark VNAR domain. [PDF]
MAbs, 2012 Advances in recombinant antibody technology and protein engineering have provided the opportunity to reduce antibodies to their smallest binding domain components and have concomitantly driven the requirement for devising strategies to increase serum half-life to optimise drug exposure, thereby increasing therapeutic efficacy. In this study, we adopted
Müller MR +15 more
europepmc +4 more sources
Small Methods, Volume 6, Issue 7, July 20, 2022., 2022 Shark‐derived vnarbodies 20G6 and 17F6 broadly neutralize SARS‐CoV‐2 Wuhan, Alpha, Beta, Kappa, Delta, Delta‐plus, Lambda variants, and sarbecovirus coronaviruses, but are unexpectedly ineffective for Omicron. Intranasal administration of 20G6 confers effective preventive and therapeutic effects in mice infected with SARS‐CoV‐2 Wuhan and Beta variant ...
Bo Feng +21 more
wiley +1 more source