A novel shark single-domain antibody targeting OGT as a tool for detection and intracellular localization [PDF]
Frontiers in Immunology, 2023 IntroductionO-GlcNAcylation is a type of reversible post-translational modification on Ser/Thr residues of intracellular proteins in eukaryotic cells, which is generated by the sole O-GlcNAc transferase (OGT) and removed by O-GlcNAcase (OGA).
Xiaozhi Xi +21 more
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Marine Drugs, 2023 Immunoglobulin new antigen receptor (IgNAR) is a naturally occurring antibody that consists of only two heavy chains with two independent variable domains.
Xierui Jiang +3 more
doaj +3 more sources
Shark Variable New Antigen Receptor (VNAR) Single Domain Antibody Fragments: Stability and Diagnostic Applications [PDF]
Antibodies, 2013 The single variable new antigen receptor domain antibody fragments (VNARs) derived from shark immunoglobulin new antigen receptor antibodies (IgNARs) represent some of the smallest known immunoglobulin-based protein scaffolds.
Stewart Nuttall +11 more
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Shark IgNAR: The Next Broad Application Antibody in Clinical Diagnoses and Tumor Therapies? [PDF]
Marine Drugs, 2023 Antibodies represent a relatively mature detection means and serve as therapeutic drug carriers in the clinical diagnosis and treatment of cancer—among which monoclonal antibodies (mAbs) currently occupy a dominant position.
Xiaofeng Jiang +5 more
doaj +2 more sources
Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library [PDF]
Malaria Journal, 2018 Background Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (
Chiuan Herng Leow +5 more
doaj +6 more sources
Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis [PDF]
Frontiers in Immunology, 2017 Induced costimulatory ligand (ICOSL) plays an important role in the activation of T cells through its interaction with the inducible costimulator, ICOS. Suppression of full T cell activation can be achieved by blocking this interaction and has been shown
Marina Kovaleva +5 more
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Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark [PDF]
Marine DrugsHeavy chain-only antibodies in sharks are called immunoglobulin new antigen receptors (IgNAR), consisting of one variable region (VNAR) and five constant regions (C1-C5).
Reo Uemura +8 more
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Humanization of the Shark V<sub>NAR</sub> Single Domain Antibody Using CDR Grafting. [PDF]
Curr Protoc, 2023 AbstractThe variable domain of the new antigen receptor (VNAR) of shark single domain antibodies is evolutionarily distant from the variable regions (VH) of mammalian immunoglobulins, yet it still has complementarity‐determining regions (CDRs) that are involved in antigen recognition, therefore making it possible to humanize by grafting these CDRs to ...
Zhang YF, Sun Y, Hong J, Ho M.
europepmc +3 more sources
Improving the pharmacokinetic properties of biologics by fusion to an anti-HSA shark VNAR domain. [PDF]
MAbs, 2012 Advances in recombinant antibody technology and protein engineering have provided the opportunity to reduce antibodies to their smallest binding domain components and have concomitantly driven the requirement for devising strategies to increase serum half-life to optimise drug exposure, thereby increasing therapeutic efficacy. In this study, we adopted
Müller MR +15 more
europepmc +4 more sources
Small Methods, Volume 6, Issue 7, July 20, 2022., 2022 Shark‐derived vnarbodies 20G6 and 17F6 broadly neutralize SARS‐CoV‐2 Wuhan, Alpha, Beta, Kappa, Delta, Delta‐plus, Lambda variants, and sarbecovirus coronaviruses, but are unexpectedly ineffective for Omicron. Intranasal administration of 20G6 confers effective preventive and therapeutic effects in mice infected with SARS‐CoV‐2 Wuhan and Beta variant ...
Bo Feng +21 more
wiley +1 more source