Results 11 to 20 of about 564 (139)

A novel shark VNAR antibody-based immunotoxin targeting TROP-2 for cancer therapy. [PDF]

Acta Pharm Sin B
TROP-2, a tumor-associated antigen, has been implicated in the progression of various epithelial tumors. Due to its favorable expression profile, TROP-2 has emerged as a promising target for antibody-drug conjugates (ADCs) based anti-tumor therapies. Although ADCs have shown efficacy in cancer treatment, their application in solid tumors is hindered by
Xi X, Wang Y, An G, Feng S, Zhu Q, Wu Z, Chen J, Zuo Z, Wang Q, Wang MW, Gu Y.   +10 more
europepmc   +5 more sources

Shark Variable New Antigen Receptor (VNAR) Single Domain Antibody Fragments: Stability and Diagnostic Applications [PDF]

Antibodies, 2013
The single variable new antigen receptor domain antibody fragments (VNARs) derived from shark immunoglobulin new antigen receptor antibodies (IgNARs) represent some of the smallest known immunoglobulin-based protein scaffolds.
Stewart Nuttall, Matthew A. Perugini, Iveth González, Joanne L. Casey, Abdulmonem Sanalla, Miles Barraclough, Con Dogovski, Julie Angerosa, Kathy Parisi, Olan Dolezal, Katherine Griffiths, Michael Foley   +11 more
doaj   +4 more sources

The influence of antibody humanization on shark variable domain (VNAR) binding site ensembles

Frontiers in Immunology, 2022
Sharks and other cartilaginous fish produce new antigen receptor (IgNAR) antibodies, as key part of their humoral immune response and are the phylogenetically oldest living organisms that possess an immunoglobulin (Ig)-based adaptive immune system. IgNAR
Monica L. Fernández-Quintero, Anna-Lena M. Fischer, Janik Kokot, Franz Waibl, Clarissa A. Seidler, Klaus R. Liedl   +5 more
doaj   +5 more sources

Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library [PDF]

Malaria Journal, 2018
Background Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (
Chiuan Herng Leow, Katja Fischer, Chiuan Yee Leow, Katleen Braet, Qin Cheng, James McCarthy   +5 more
doaj   +6 more sources

Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis [PDF]

Frontiers in Immunology, 2017
Induced costimulatory ligand (ICOSL) plays an important role in the activation of T cells through its interaction with the inducible costimulator, ICOS. Suppression of full T cell activation can be achieved by blocking this interaction and has been shown
Marina Kovaleva, Katherine Johnson, John Steven, Caroline J. Barelle, Andrew Porter, Andrew Porter   +5 more
doaj   +6 more sources

Mechanisms of SARS-CoV-2 neutralization by shark variable new antigen receptors elucidated through X-ray crystallography [PDF]

Nature Communications, 2021
Shark antibodies (Variable New Antigen Receptors, VNARs) are the smallest naturally occurring antibody fragments. Here, the authors screen a VNAR phage display library against the SARS-CoV2 receptor binding domain (RBD) and identify VNARs that neutralize
Obinna C. Ubah, Eric W. Lake, Gihan S. Gunaratne, Joseph P. Gallant, Marie Fernie, Austin J. Robertson, Jonathan S. Marchant, Tyler D. Bold, Ryan A. Langlois, William E. Matchett, Joshua M. Thiede, Ke Shi, Lulu Yin, Nicholas H. Moeller, Surajit Banerjee, Laura Ferguson, Marina Kovaleva, Andrew J. Porter, Hideki Aihara, Aaron M. LeBeau, Caroline J. Barelle   +20 more
doaj   +2 more sources

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica) [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda, Tatsuhiko Ozawa, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki   +13 more
doaj   +2 more sources

Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei) [PDF]

Frontiers in Marine Science, 2023
Elasmobranchs are crucial for comparative studies of evolution, as they belong to the most ancient vertebrate lineages that survived numerous extinction events and persist until today.
Jianqing Wen, Jianqing Wen, Jinyu Gong, Jinyu Gong, Pengwei Li, Penghui Deng, Penghui Deng, Mengsi Sun, Yujie Wu, Chenxi Tian, Chenxi Tian, Hao Wang, Yunchen Bi, Yunchen Bi, Yunchen Bi   +14 more
doaj   +3 more sources

Identification and characterization of shark VNARs targeting the Helicobacter pylori adhesin HpaA

Artificial Cells, Nanomedicine, and Biotechnology, 2023
Helicobacter pylori (H. pylori) is recognized as a pathogen associated with several gastrointestinal diseases. The current treatments exhibit numerous drawbacks, including antibiotic resistance. H.
Yanchun Gao, Ruihong Wang, Lin Liu, Shitao Feng, Xiaozhi Xi, Wengong Yu, Yuchao Gu, Ye Wang   +7 more
doaj   +3 more sources

VNARs: An Ancient and Unique Repertoire of Molecules That Deliver Small, Soluble, Stable and High Affinity Binders of Proteins [PDF]

Antibodies, 2015
At 420 million years, the variable domain of New Antigen Receptors or VNARs are undoubtedly the oldest (and smallest) antigen binding single domains identified in the vertebrate kingdom.
Caroline Barelle, Andy Porter
doaj   +5 more sources